scholarly journals Detection of Giardia lamblia,Entamoeba histolytica/Entamoeba dispar, andCryptosporidium parvum Antigens in Human Fecal Specimens Using the Triage Parasite Panel Enzyme Immunoassay

2000 ◽  
Vol 38 (9) ◽  
pp. 3337-3340 ◽  
Author(s):  
Lynne S. Garcia ◽  
Robyn Y. Shimizu ◽  
Caroline N. Bernard
Keyword(s):  
Enzyme Immunoassay ◽  
Entamoeba Histolytica ◽  
Cryptosporidium Parvum ◽  
Giardia Lamblia ◽  
Gold Standard ◽  
Cross Reactivity ◽  
Entamoeba Dispar ◽  
Specific Antibodies ◽  
Fresh Frozen ◽  
Stool Specimens

The Triage parasite panel (BIOSITE Diagnostics, San Diego, Calif.) is a new qualitative enzyme immunoassay (EIA) panel for the detection of Giardia lamblia, Entamoeba histolytica/E. dispar, and Cryptosporidium parvum in fresh or fresh, frozen, unfixed human fecal specimens. By using specific antibodies, antigens specific for these organisms are captured and immobilized on a membrane. Panel performance was evaluated with known positive and negative stool specimens (a total of 444 specimens) that were tested by the standard ova and parasite (O&P) examination as the “gold standard,” including staining with both trichrome and modified acid-fast stains. Specimens with discrepant results between the reference and Triage methods were retested by a different method, either EIA or immunofluorescence. A number of samples with discrepant results with the Triage device were confirmed to be true positives. After resolution of discrepant results, the number of positive specimens and the sensitivity and specificity results were as follows: for G. lamblia, 170, 95.9%, and 97.4%, respectively; for E. histolytica/E. dispar, 99, 96.0%, and 99.1%, respectively; and for C. parvum, 60, 98.3%, and 99.7%, respectively. There was no cross-reactivity with other parasites found in stool specimens, including eight different protozoa (128 challenges) and three different helminths (83 challenges). The ability to perform the complete O&P examination should remain an option for those patients with negative parasite panel results but who are still symptomatic.

scholarly journals Diagnosis of Amebic Liver Abscess and Intestinal Infection with the TechLab Entamoeba histolytica II Antigen Detection and Antibody Tests

2000 ◽  
Vol 38 (9) ◽  
pp. 3235-3239 ◽  
Author(s):  
Rashidul Haque ◽  
Nasir Uddin Mollah ◽  
Ibne Karim M. Ali ◽  
Khorshed Alam ◽  
Aleida Eubanks ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Positive Culture ◽  
Antigen Detection ◽  
Pcr Analysis ◽  
Amebic Liver Abscess ◽  
Intestinal Infection ◽  
Entamoeba Dispar ◽  
Stool Specimens ◽  
Culture Negative

A noninvasive diagnostic test for amebic liver abscess is needed, because amebic and bacterial abscesses appear identical on ultrasound or computer tomography and because it is rarely possible to identifyEntamoeba histolytica in stool specimens from patients with amebic liver abscess. Here we report a method of detection in serum of circulating E. histolytica Gal/GalNAc lectin to diagnose amebic liver abscess, which was used in patients from Dhaka, Bangladesh. The TechLab E. histolytica II test (which differentiates the true pathogen E. histolytica fromEntamoeba dispar) detected Gal/GalNAc lectin in the sera of 22 of 23 (96%) amebic liver abscess patients tested prior to treatment with the antiamebic drug metronidazole and 0 of 70 (0%) controls. After 1 week of treatment with metronidazole, 9 of 11 (82%) patients became serum lectin antigen negative. The sensitivity of the E. histolytica II antigen detection test for intestinal infection was also evaluated. Antigen detection identified E. histolytica infection in 50 samples from 1,164 asymptomatic preschool children aged 2 to 5 years, including 16 of 16 (100%) culture-positive specimens. PCR analysis of stool specimens was used to confirm that most antigen-positive but culture-negative specimens were true-positive: PCR identified parasite DNA in 27 of 34 (79%) of the antigen-positive, culture-negative stool specimens. Antigen detection was a more sensitive test for infection than antilectin antibodies, which were detected in only 76 of 98 (78%) amebic liver abscess patients and in 26 of 50 (52%) patients with intestinal infection. We conclude that the TechLab E. histolytica II kit is a sensitive means to diagnose hepatic and intestinal amebiasis prior to the institution of metronidazole treatment.

scholarly journals Prevalence of Entamoeba histolytica and Giardia lamblia among schoolchildren in Um-Asher Area, Sudan

2020 ◽  
Author(s):  
Khalid Hajissa ◽  
Abd Elhafiz M.A. Muhajir ◽  
Tmador Abd All ◽  
M.S. Zakeia ◽  
Hamza Adam Eshag ◽  
...  
Keyword(s):  
Public Health ◽  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Primary Schools ◽  
Gastrointestinal Parasites ◽  
Health Concern ◽  
Health Issues ◽  
School Aged Children ◽  
Stool Specimens ◽  
Fresh Stool

Abstract Objective: Infections with Entamoeba histolytica and Giardia lamblia are widely prevalent and responsible for serious public health issues among school-aged children in developing countries. This study was conducted to determine the prevalence of these parasites among children from two primary schools in Um-Asher area, Khartoum, Sudan. A total 170 fresh stool specimens were collected from November 2017 to June 2018. The samples were examined by microscopy for the presence of the two parasites. Results: The overall prevalence of both parasites among the 170 children was 20%, with 13 children infected with E. histolytica (7.6%) and 23 with G. lamblia (13.5%). Most of the positive cases were single infections. Only two children (1.2%) had mixed infections. This indicates that these gastrointestinal parasites remain a challenging public health concern wherever sanitation and health measures are limited.

Prevalência de enteroparasitoses em pacientes atendidos em uma unidade mista de saúde de Beneditinos – PI

2017 ◽  
Vol 2 (1) ◽  
Author(s):  
Joao Lopes Sampaio ◽  
Veruska Cavalcanti Barros
Keyword(s):  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Strongyloides Stercoralis ◽  
Entamoeba Dispar

As enteroparasitoses pertencem ao grupo de doenças negligenciadas, contribuindo para elevadas taxas de morbidade e mortalidade, principalmente, nos países em desenvolvimento. Este estudo avaliou a prevalência de enteroparasitoses em pacientes atendidos em unidade pública de saúde do município Beneditinos - PI. Foram analisados 116 laudos parasitológicos, dos quais foram coletados dados sobre gênero e idade. Do total, 14 laudos (12,2%) apresentaram resultado positivo para alguma enteroparasitose. As maiores prevalências foram observadas em crianças na idade escolar e adultos acima de 56 anos. Os parasitos identificados foram: Giardia lamblia, Entamoeba histolytica/Entamoeba dispar, Entamoeba coli, Endolimax nana e Strongyloides stercoralis. Esses resultados estão em consonância com outros estudos, nos quais as precárias condições sanitárias, maus hábitos de higiene e a desinformação em relação às parasitoses potencializam a transmissão desses parasitas. Apesar da baixa prevalência encontrada, os fatores de risco relacionados à transmissão das enteroparasitoses podem variar conforme condições higiênico-sanitárias e socioculturais, causando impactos importantes no custeio da saúde pública e no bem-estar da população. Portanto, medidas de prevenção devem ser asseguradas, como a melhoria das condições socioeconômicas, de saneamento básico e da educação em saúde.

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