scholarly journals Comparative Pathobiology of the Intestinal Protozoan Parasites Giardia lamblia, Entamoeba histolytica, and Cryptosporidium parvum

Pathogens ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 116 ◽  
Author(s):  
Hemphill ◽  
Müller ◽  
Müller
Keyword(s):  
Entamoeba Histolytica ◽  
Cryptosporidium Parvum ◽  
Giardia Lamblia ◽  
Intestinal Tract ◽  
Protozoan Parasites ◽  
Intestinal Protozoan ◽  
Colon Wall ◽  
Causative Agents ◽  
Life Threatening ◽  
Human Intestinal Tract

Protozoan parasites can infect the human intestinal tract causing serious diseases. In the following article, we focused on the three most prominent intestinal protozoan pathogens, namely, Giardia lamblia, Entamoeba histolytica, and Cryptosporidium parvum. Both C. parvum and G. lamblia colonize the duodenum, jejunum, and ileum and are the most common causative agents of persistent diarrhea (i.e., cryptosporidiosis and giardiasis). Entamoeba histolytica colonizes the colon and, unlike the two former pathogens, may invade the colon wall and disseminate to other organs, mainly the liver, thereby causing life-threatening amebiasis. Here, we present condensed information concerning the pathobiology of these three diseases.

scholarly journals Intestinal Protozoan Parasitosis in 4- and 11-year-old Children in Cunduana Community, Riobamba Canton, Chimborazo, June 2019 June

2021 ◽  
Author(s):  
Silvia Marcela Inca Martínez ◽  
Martha Cecilia Bonilla Caicedo ◽  
Silvana Paola Ocaña Coello
Keyword(s):  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Intellectual Development ◽  
Public Health Problem ◽  
Oral Route ◽  
School Age Children ◽  
Intestinal Protozoan ◽  
Intestinal Protozoa ◽  
Intestinal Parasitosis ◽  
Physiological Serum

Introduction: Parasitosis is a public health problem that affects millions of people, school-age children are more vulnerable, affects their physical and intellectual development, are transmitted by fecal-oral route, and is associated with poor hygienic and sanitary conditions. In Ecuador they constitute the second reason for medical consultation. Objective: To contribute to the epidemiological knowledge of intestinal parasitosis by protozoa in our environment. Methodology: An experimental descriptive study, with a universe of 80 children, obtaining 55 samples, a direct examination was carried out in preparations with physiological serum and lugol, identifying the parasites by their microscopic morphology. Results: The prevalence of intestinal parasitosis by protozoa was 78.18%, while for the parasites Endolimax nana 30.9% Entamoeba histolytica, 34.5% and Giardia lamblia 14.5%. 40%  of the participants were monoparasitized and 38.2% had polyparasitosis. Conclusion: High prevalence of parasitosis by intestinal protozoa was determined in the children of the Unit with 78.18%. The most prevalent ameboid protozoan was E. nana, followed by the pathogenic protozoan E. histolytica, while G. lamblia was flagellated protozoan identified. The lack of adequate sanitary infrastructure, habits of hygiene, environmental pollution, influence the prevalence of intestinal parasitosis. Keywords: protozoan infections, intestinal diseases, child. RESUMEN Introducción: Parasitosis es un problema de salud pública que afecta a millones de personas, los niños en edad escolar son más vulnerables, afecta su desarrollo físico e intelectual, se transmiten por vía fecal-oral, y está asociada a deficientes condiciones higiénicas y sanitarias. En Ecuador constituyen el segundo motivo de consulta médica. Objetivo: contribuir al conocimiento epidemiológico de las parasitosis intestinales por protozoos en nuestro medio. Metodología: estudio descriptivo experimental, con un universo de 80 niños y niñas, obteniendo 55 muestras, se realizó examen directo en preparaciones con suero fisiológico y lugol, identificando los parásitos por su morfología microscópica. Resultados: La prevalencia de parasitosis intestinal por protozoos fue de 78,18%, mientras que para los parásitos Endolimax nana 30,9%, Entamoeba histolytica 34,5% y Giardia lamblia 14,5%. El 40% de los participantes fue monoparasitado y el 38,2% presentó poliparasitosis. Conclusiones: Se determinó alta prevalencia de parasitosis por protozoos intestinales en los niños y niñas de la Unidad con un 78,18%. El protozoo ameboideo de mayor prevalencia fue E. nana, seguido del protozoo patógeno E. histolytica, mientras que G. lambia fue protozoo flagelado identificado. La falta de una adecuada infraestructura sanitaria, hábitos de higiene, contaminación ambiental, influyen en la prevalencia de la parasitosis intestinal. Palabras clave: infecciones por protozoos, parasitosis intestinales, niños.

scholarly journals Virtual Screening of FDA-Approved Drugs against Triose Phosphate Isomerase from Entamoeba histolytica and Giardia lamblia Identifies Inhibitors of Their Trophozoite Growth Phase

2021 ◽  
Vol 22 (11) ◽  
pp. 5943
Author(s):  
Alfredo Juárez-Saldivar ◽  
Elizabeth Barbosa-Cabrera ◽  
Edgar E. Lara-Ramírez ◽  
Alma D. Paz-González ◽  
Ana V. Martínez-Vázquez ◽  
...  
Keyword(s):  
Virtual Screening ◽  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Triosephosphate Isomerase ◽  
Glycolytic Enzyme ◽  
Public Health Issue ◽  
Intestinal Protozoan ◽  
Approved Drugs ◽  
Fda Approved Drugs

Infectious diseases caused by intestinal protozoan, such as Entamoeba histolytica (E. histolytica) and Giardia lamblia (G. lamblia) are a worldwide public health issue. They affect more than 70 million people every year. They colonize intestines causing primarily diarrhea; nevertheless, these infections can lead to more serious complications. The treatment of choice, metronidazole, is in doubt due to adverse effects and resistance. Therefore, there is a need for new compounds against these parasites. In this work, a structure-based virtual screening of FDA-approved drugs was performed to identify compounds with antiprotozoal activity. The glycolytic enzyme triosephosphate isomerase, present in both E. histolytica and G. lamblia, was used as the drug target. The compounds with the best average docking score on both structures were selected for the in vitro evaluation. Three compounds, chlorhexidine, tolcapone, and imatinib, were capable of inhibit growth on G. lamblia trophozoites (0.05–4.935 μg/mL), while folic acid showed activity against E. histolytica (0.186 μg/mL) and G. lamblia (5.342 μg/mL).

Blastocystosis

2011 ◽  
Author(s):  
Manoj K. Puthia ◽  
Kevin S. Tan
Keyword(s):  
Human Disease ◽  
Intestinal Tract ◽  
Clinical Symptoms ◽  
Epidemiological Studies ◽  
Watery Diarrhoea ◽  
Intestinal Protozoan ◽  
Potential Pathogen ◽  
Stool Specimens ◽  
Human Intestinal Tract

Blastocystis , the causative agent of blastocystosis, is an intestinal protozoan commonly identified in stool specimens of patients. It is one of the most common parasites inhabiting the human intestinal tract. Clinical symptoms attributed to Blastocystis include recurrent watery diarrhoea, mucous diarrhoea, vomiting, abdominal cramps and flatulence. Blastocystis infects both children and adults and its geographical distribution appears to be global with prevalence ranging from 30 to 50% in developing countries (Stenzel and Boreham 1996).Blastocystis was first described as a distinct organism in 1911 and the name B. enterocola was proposed for this organism (Alexeieff 1911). It was isolated from human faeces and the name B. hominis was coined (Brumpt 1912). At first, it was described as a harmless intestinal yeast and ignored for many decades. Its association with human disease was suggested by a number of reports and eventually work by Zierdt (1991) increased the awareness of Blastocystis infections in humans.In spite of its description about a century ago, the exact role of Blastocystis as a cause of human disease is uncertain. A number of clinical and epidemiological studies implicate the parasite as a potential pathogen (Al-Tawil et al. 1994; El-Shazly et al. 2005; Garavelli et al. 1991; Logar et al. 1994) while others exonerate it as an etiology of intestinal disease (Chen et al. 2003; Leder et al. 2005; Shlim et al. 1995). Significant progress has been achieved on descriptions of the morphology and genetic diversity of Blastocystis but most aspects of its life cycle, molecular biology, and athogenicity remain unresolved (Stenzel and Boreham 1996; Tan 2004).

Crosstalk between Entamoeba histolytica and the human intestinal tract during amoebiasis

Parasitology ◽  
2017 ◽  
Vol 146 (9) ◽  
pp. 1140-1149 ◽  
Author(s):  
Elisabeth Labruyère ◽  
Roman Thibeaux ◽  
Jean-Christophe Olivo-Marin ◽  
Nancy Guillén
Keyword(s):  
Entamoeba Histolytica ◽  
Intestinal Tract ◽  
Colonic Mucosa ◽  
Protozoan Parasite ◽  
Mortality Rates ◽  
High Morbidity ◽  
Mucus Layer ◽  
Lytic Enzymes ◽  
Microbial Agent ◽  
Human Intestinal Tract

AbstractThe protozoan parasite Entamoeba histolytica is the microbial agent of amoebiasis – an infection that is endemic worldwide and is associated with high morbidity and mortality rates. As the disease develops, virulent E. histolytica deplete the mucus layer, interact with the intestinal epithelium, and then degrade the colonic mucosa and disrupt the extracellular matrix (ECM). Our research demonstrated that virulent parasites with an invasive phenotype display rapid, highly specific changes in their transcriptome (notably for essential factors involved in carbohydrate metabolism and the processing of glycosylated residues). Moreover, combined activation of parasite and host lytic enzymes leads to the destruction of the intestinal parenchyma. Together, these enzymes degrade the mucus layer and the ECM, and trigger the inflammatory response essential to the development of amoebiasis.

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