scholarly journals Green UV Absorbance Ratio Method for Determination of Eprosartan and Hydrochlorothiazide

2021 ◽  
Vol 5 (1) ◽  
pp. 90-99
Author(s):  
Khaled Marghany ◽  
Randa Abdel Salam ◽  
Ghada Hadad ◽  
Elsayed Ibrahim
Keyword(s):  
Ratio Method ◽  
Uv Absorbance ◽  
Absorbance Ratio

Unveiling heme proteins conformational stability through a UV absorbance ratio method

2007 ◽  
Vol 371 (2) ◽  
pp. 253-255 ◽  
Author(s):  
Luis M.A. Oliveira ◽  
Carlos Cordeiro ◽  
Ana Ponces Freire ◽  
Carla Ascenso ◽  
Alexandre Quintas
Keyword(s):  
Heme Proteins ◽  
Conformational Stability ◽  
Ratio Method ◽  
Uv Absorbance ◽  
Absorbance Ratio

Spectroscopic Determination of Aspirin and Omeprazole by Absorbance Ratio and Multicomponent Mode Method

2017 ◽  
Vol 10 (6) ◽  
pp. 3900-3905
Author(s):  
Hajera Khan ◽  
Swapna S Bandewar ◽  
Mohammad Zameeruddin ◽  
Vishvanath B Bharkad
Keyword(s):  
Accurate Method ◽  
Analysis Data ◽  
Spectroscopic Determination ◽  
Ratio Method ◽  
Analysis Method ◽  
Isobestic Point ◽  
Absorbance Ratio ◽  
Ich Guidelines ◽  
Mode Method

Here we describe a simple, rapid and accurate method for simultaneous assay of aspirin and omeprazole. The first method was Absorbance ratio method (Method 1) and second method was multi component mode method of analysis (Method 2).  Methanol: water (8:2) was used as solvent for both methods, using 293 nm as isobestic point for absorbance ratio method. The wavelength ranges 275.80 nm for aspirin and 302.20 nm for omeprazole for method 2, which represents the absorbance maxima of both drugs respectively. Beer’s law was applied in the concentration ranges of 2-14μg/mL and 2-18 μg/mL for aspirin and omeprazole, respectively, in absorbance ratio methods. The percentage assay was found to be in the range 99.74 to 100 % for aspirin and 99.69 to 99.9 % for omeprazole for both the methods. Recovery was found in the range of 99.74 –100.14 % for aspirin and omeprazole for both methods. The analysis data has been validated statistically and recovery studies confirmed the accuracy and reproducibility of the proposed methods, which were carried out according to the ICH guidelines.     

scholarly journals Quantitative determination of weak acids, weak bases and their salts by the absorbance ratio method

1968 ◽  
Vol 17 (6) ◽  
pp. 683-703
Author(s):  
Bunzo TAMURA ◽  
Ikuyo FURUYAMA ◽  
Kazuyuki MORIURA
Keyword(s):  
Quantitative Determination ◽  
Ratio Method ◽  
Weak Acids ◽  
Weak Bases ◽  
Absorbance Ratio

SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF DAPAGLIFLOZIN AND SAXAGLIPTIN IN FIXED DOSE COMBINATION (FDC) BY Q-ABSORBANCE RATIO METHOD

INDIAN DRUGS ◽  
2021 ◽  
Vol 57 (11) ◽  
pp. 63-75
Author(s):  
Gan Ee How ◽  
◽  
Venkata Subrahmanya Lokesh Bontha
Keyword(s):  
Uv Spectra ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Ratio Method ◽  
Isosbestic Point ◽  
Good Precision ◽  
Absorbance Ratio ◽  
Ich Guidelines ◽  
Dose Combination

A simple Q-absorbance ratio method have been developed for the determination of dapagliflozin (DAPA) and saxagliptin (SAXA) in fixed dose combination (FDC) using UV-Visible spectrophotometer. In this method, the UV spectra of DAPA and SAXA were overlaid to obtain wavelength at isosbestic point (λiso) of 217.6 nm and at absorption maximum (λmax) of DAPA at 224.2 nm, which are involved in the formation of Q-absorbance equation. Validation of method was done according to ICH guidelines. DAPA and SAXA obeyed Beers law in the concentration range of 2-25 µg/mL and 5-25 µg/mL, respectively. Good accuracy of method was determined by recovery studies and found to be in the range of 103.1-104.6% for DAPA and 97.7-102.4% for SAXA. This method has shown good precision (%RSD < 2.0). Statistical analysis like one-way ANOVA and student t-test were conducted and the reported method was accurate. This method was found to be simple, cheap, eco-friendly accurate and precise and can be used for routine analysis of DAPA and SAXA in FDC for testing regularly in manufacturing units.

Extractive Spectrophotometric Method for the Determination of Glibenclamide by Ion-Pair Complex in Pure Form and Pharmaceutical Formulation

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
RUAA MUAYAD MAHMOOD ◽  
HAMSA MUNAM YASSEN ◽  
SAMAR , NAJWA ISSAC ABDULLA AHMED DARWEESH ◽  
NAJWA ISSAC ABDULLA
Keyword(s):  
Spectrophotometric Method ◽  
Molar Absorptivity ◽  
Ion Pair ◽  
Pharmaceutical Formulation ◽  
Ratio Method ◽  
Colored Complex ◽  
Colored Product ◽  
Method Of Continuous Variation ◽  
Job's Method

Simple, rapid and sensitive extractive spectrophotometric method is presented for the determination of glibenclamide (Glb) based on the formation of ion-pair complex between the Glb and anionic dye, methyl orange (MO) at pH 4. The yellow colored complex formed was quantitatively extracted into dichloromethane and measured at 426 nm. The colored product obeyed Beer’s law in the concentration range of (0.5-40) μg.ml-1. The value of molar absorptivity obtained from Beer’s data was found to be 31122 L.mol-1.cm-1, Sandell’s sensitivity value was calculated to be 0.0159 μg.cm-2, while the limits of detection (LOD) and quantification (LOQ) were found to be 0.1086 and 0.3292 μg.ml-1, respectively. The stoichiometry of the complex created between the Glb and MO was 1:1 as determined via Job’s method of continuous variation and mole ratio method. The method was successfully applied for the analysis of pharmaceutical formulation.

Comparative Study for the Assay of Plant Derived Chemicals in Pharmaceutical Formulation Using Methods Dependent on Factorized Spectra

2021 ◽  
Author(s):  
Nesma M Fahmy ◽  
Adel M Michael
Keyword(s):  
Pharmaceutical Formulations ◽  
Pharmaceutical Formulation ◽  
Ternary Mixtures ◽  
Ratio Method ◽  
The Novel ◽  
Naturally Occurring ◽  
Accuracy And Precision ◽  
Validation Parameters ◽  
Significant Difference

Abstract Background Modern built-in spectrophotometer software supporting mathematical processes provided a solution for increasing selectivity for multicomponent mixtures. Objective Simultaneous spectrophotometric determination of the three naturally occurring antioxidants—rutin(RUT), hesperidin(HES), and ascorbic acid(ASC)—in bulk forms and combined pharmaceutical formulation. Method This was achieved by factorized zero order method (FZM), factorized derivative method (FD1M), and factorized derivative ratio method (FDRM), coupled with spectrum subtraction(SS). Results Mathematical filtration techniques allowed each component to be obtained separately in either its zero, first, or derivative ratio form, allowing the resolution of spectra typical to the pure components present in Vitamin C Forte® tablets. The proposed methods were applied over a concentration range of 2–50, 2–30, and 10–100 µg/mL for RUT, HES, and ASC, respectively. Conclusions Recent methods for the analysis of binary mixtures, FZM and FD1M, were successfully applied for the analysis of ternary mixtures and compared to the novel FDRM. All were revealed to be specific and sensitive with successful application on pharmaceutical formulations. Validation parameters were evaluated in accordance with the International Conference on Harmonization guidelines. Statistical results were satisfactory, revealing no significant difference regarding accuracy and precision. Highlights Factorized methods enabled the resolution of spectra identical to those of pure drugs present in mixtures. Overlapped spectra of ternary mixtures could be resolved by spectrum subtraction coupled FDRM (SS-FDRM) or by successive application of FZM and FD1M.

Preliminary Studies on the Absorbance Ratio Method Used to Determining the Age of Stamp‐pad Ink Seal

2019 ◽  
Vol 64 (4) ◽  
pp. 1203-1212
Author(s):  
Guoliang Ouyang ◽  
Biao Li ◽  
Pengnan Zhao ◽  
Xingfei Guo ◽  
Changliang Wang
Keyword(s):  
Ratio Method ◽  
Absorbance Ratio
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