Spectroscopic Determination of Aspirin and Omeprazole by Absorbance Ratio and Multicomponent Mode Method

2017 ◽  
Vol 10 (6) ◽  
pp. 3900-3905
Author(s):  
Hajera Khan ◽  
Swapna S Bandewar ◽  
Mohammad Zameeruddin ◽  
Vishvanath B Bharkad
Keyword(s):  
Accurate Method ◽  
Analysis Data ◽  
Spectroscopic Determination ◽  
Ratio Method ◽  
Analysis Method ◽  
Isobestic Point ◽  
Absorbance Ratio ◽  
Ich Guidelines ◽  
Mode Method

Here we describe a simple, rapid and accurate method for simultaneous assay of aspirin and omeprazole. The first method was Absorbance ratio method (Method 1) and second method was multi component mode method of analysis (Method 2).  Methanol: water (8:2) was used as solvent for both methods, using 293 nm as isobestic point for absorbance ratio method. The wavelength ranges 275.80 nm for aspirin and 302.20 nm for omeprazole for method 2, which represents the absorbance maxima of both drugs respectively. Beer’s law was applied in the concentration ranges of 2-14μg/mL and 2-18 μg/mL for aspirin and omeprazole, respectively, in absorbance ratio methods. The percentage assay was found to be in the range 99.74 to 100 % for aspirin and 99.69 to 99.9 % for omeprazole for both the methods. Recovery was found in the range of 99.74 –100.14 % for aspirin and omeprazole for both methods. The analysis data has been validated statistically and recovery studies confirmed the accuracy and reproducibility of the proposed methods, which were carried out according to the ICH guidelines.     

SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF DAPAGLIFLOZIN AND SAXAGLIPTIN IN FIXED DOSE COMBINATION (FDC) BY Q-ABSORBANCE RATIO METHOD

INDIAN DRUGS ◽  
2021 ◽  
Vol 57 (11) ◽  
pp. 63-75
Author(s):  
Gan Ee How ◽  
◽  
Venkata Subrahmanya Lokesh Bontha
Keyword(s):  
Uv Spectra ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Ratio Method ◽  
Isosbestic Point ◽  
Good Precision ◽  
Absorbance Ratio ◽  
Ich Guidelines ◽  
Dose Combination

A simple Q-absorbance ratio method have been developed for the determination of dapagliflozin (DAPA) and saxagliptin (SAXA) in fixed dose combination (FDC) using UV-Visible spectrophotometer. In this method, the UV spectra of DAPA and SAXA were overlaid to obtain wavelength at isosbestic point (λiso) of 217.6 nm and at absorption maximum (λmax) of DAPA at 224.2 nm, which are involved in the formation of Q-absorbance equation. Validation of method was done according to ICH guidelines. DAPA and SAXA obeyed Beers law in the concentration range of 2-25 µg/mL and 5-25 µg/mL, respectively. Good accuracy of method was determined by recovery studies and found to be in the range of 103.1-104.6% for DAPA and 97.7-102.4% for SAXA. This method has shown good precision (%RSD < 2.0). Statistical analysis like one-way ANOVA and student t-test were conducted and the reported method was accurate. This method was found to be simple, cheap, eco-friendly accurate and precise and can be used for routine analysis of DAPA and SAXA in FDC for testing regularly in manufacturing units.

scholarly journals Absorbance Correction Method for Simultaneous Estimation of Nifedipine and Metoprolol Succinate in Their Synthetic Mixture Using From Spectrophotometry

2015 ◽  
Vol 1 (3) ◽  
pp. 151
Author(s):  
Sojitra Rajanit ◽  
Paras Virani ◽  
Hashumati Raj
Keyword(s):  
Accurate Method ◽  
Correction Method ◽  
Synthetic Mixture ◽  
Simultaneous Estimation ◽  
Metoprolol Succinate ◽  
Absorption Correction ◽  
Ich Guidelines ◽  
Development And Validation ◽  
Tablet Dosage

A new simple, economical, precise and accurate method are described for the simultaneous determination of Nifedipine (NIF) and Metoprolol Succinate (MET) in combined tablet dosage form. The proposed method was applied for the determination of Nifedipine and Metoprolol Succinate in synthetic mixture, for determination of sampling wavelength, 10?g/ml of each of NIF and MET were scanned in 200-400 nm range and sampling wavelengths were 313nm for NIF and 275.40nm for MET are selected for development and validation of absorption correction method. For this method linearity observed in the range of 5-25?g/ml for NIF and 25-125?g/ml for MET, and in their pharmaceutical formulation with mean percentage recoveries 100.68 and 100.33, respectively. The method was validated according to ICH guidelines and can be applied for routine quality control testing.

scholarly journals LC-MS/MS METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF CARDIAZOL IN HUMAN PLASMA

2019 ◽  
pp. 380-385
Author(s):  
IRYNA DRAPAK ◽  
BORYS ZIMENKOVSKY ◽  
LINA PEREKHODA ◽  
SERGIY KOVALENKO ◽  
Liliya Logoyda
Keyword(s):  
Formic Acid ◽  
Human Plasma ◽  
Method Development ◽  
Accurate Method ◽  
Sample Volume ◽  
Ich Guidelines ◽  
Coefficients Of Variation ◽  
Method Development And Validation ◽  
Development And Validation

Objective: The main purpose of this study was to develop a simple, precise, rapid and accurate method for the quantification of cardiazol in human plasma. Methods: Chromatography was achieved on Discovery C18, 50 × 2.1 mm, 5 μm column. Samples were chromatographed in a gradient mode (eluent A (acetonitrile-water–formic acid, 5: 95: 0.1 v/v), eluent B (acetonitrile–formic acid, 100: 0.1 v/v)). The initial content of the eluent B of 8%, which increases linearly to 1.0 min to 100%, is maintained up to 1.5 min and returned to the original 8% to 1.51 min. The mobile phase was delivered at a flow rate of 0.400 ml/min into the mass spectrometer ESI chamber. The sample volume was 300 μl. Results: The total chromatographic run time was 2.5 min and the elution of cardiazol and IS (difenoconazole) occurred at ~2.15 and 1.98 min, respectively. A linear response function was established at 1-100 ng/ml for cardiazol and difenoconazole in human plasma. The % mean recovery for cardiazol in LQC, MQC and HQC was 102.8 %, 100.3 % and 95.9 %. The lowest concentration with the RSD<20% was taken as LLOQ and was found to be 1.10 ng/ml for cardiazol. The % accuracy of LLOQ samples prepared with the different biological matrix lots was found 109.7 %, which were found within the range of 80.00-120.00 % for the seven different plasma lots. % CV for LLOQ samples was observed as 11.9 %, which are within 20.00% of the acceptance criteria. The within-run coefficients of variation ranged between 0.311 % and 0.601 % for cardiazol. The within-run percentages of nominal concentrations ranged between 99.80 % and 100.41 % for cardiazol. The between-run coefficients of variation ranged between 0.332 % and 0.615 % for cardiazol. The between-run percentages of nominal concentrations ranged between 98.18 % and 101.21 % for cardiazol. Conclusion: A rapid method was developed for simultaneous determination of cardiazol in human plasma. The method was strictly validated according to the ICH guidelines. Acquired results demonstrate that the proposed strategy can be effortlessly and advantageously applied for routine examination of cardiazol in human plasma.

Research on Prediction Analysis Method about the Taxi Demand of Tourist City with Seasonal Fluctuation

2013 ◽  
Vol 361-363 ◽  
pp. 2025-2029 ◽  
Author(s):  
Yan Li Jiao ◽  
Meng Ge
Keyword(s):  
Seasonal Changes ◽  
Supply And Demand ◽  
Seasonal Fluctuation ◽  
Balance Method ◽  
Ratio Method ◽  
Analysis Method ◽  
Seasonal Fluctuations ◽  
Prediction Analysis ◽  
Advantages And Disadvantages

The determination of taxi demand in tourist city is affected by seasonal changes. It presents peaks and troughs so it is difficult to be predicted. On the basis of analyzing the advantages and disadvantages of existing forecast methods, this paper puts forward modified supply and demand balance method and ratio method to forecast taxi demand and then discusses the methods of dealing with seasonal fluctuations.

scholarly journals Quantitative determination of weak acids, weak bases and their salts by the absorbance ratio method

1968 ◽  
Vol 17 (6) ◽  
pp. 683-703
Author(s):  
Bunzo TAMURA ◽  
Ikuyo FURUYAMA ◽  
Kazuyuki MORIURA
Keyword(s):  
Quantitative Determination ◽  
Ratio Method ◽  
Weak Acids ◽  
Weak Bases ◽  
Absorbance Ratio

A trace analysis method based on HPLC-MS/MS for the simultaneous determination of residues of the two isomers of pyrisoxazole in tomato fruit and soil

2016 ◽  
Vol 8 (9) ◽  
pp. 2041-2047 ◽  
Author(s):  
He Zhu ◽  
Fuyan Jia ◽  
Qi Ding ◽  
Chengtian Huang ◽  
Yang Yu ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Tomato Fruit ◽  
Accurate Method ◽  
Tandem Mass ◽  
Analysis Method ◽  
Soil P ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

A simple, sensitive and accurate method for the simultaneous determination of residues of the two isomers of pyrisoxazole in tomato fruit and soil was established using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).

scholarly journals A New Analytical Q-Absorbance Ratio Method Development and Validation for Simultaneous Estimation of Lamivudine and Isoniazid

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Gitu Pandey ◽  
Brahmeshwar Mishra
Keyword(s):  
Method Development ◽  
The Other ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Ratio Analysis ◽  
Absorption Ratio ◽  
Absorbance Ratio ◽  
Ich Guidelines ◽  
Method Development And Validation ◽  
Development And Validation

A new UV spectrophotometric absorption ratio method was developed and validated for the simultaneous estimation of lamivudine and isoniazid. The method involved Q-absorption ratio analysis using two wavelengths, with one being the λmax of lamivudine (272 nm, λ2) and the other being the isoabsorptive point of both drugs (246 nm, λ1). Beer’s law was obeyed in the concentration range between 5 and 30 µg/mL for both lamivudine and isoniazid. The results of analysis have been validated statistically and by recovery studies as per ICH guidelines. The accuracy ranged between 99.65 and 101.91% and Sandell’s sensitivity ranged between 0.0229 and 0.0347 µg/cm2. The method was found to be simple, precise, reproducible, rapid, and economical. Hence, it could be used in the analysis of laboratory samples and marketed formulations containing these two drugs in the future.

scholarly journals Simultaneous Spectrophotometric Determination of Paracetamol, Propyphenazone and Caffeine By Using Absorption Ratio Method

2018 ◽  
Vol 6 (5) ◽  
pp. 5-8
Author(s):  
Ainil Fithri Pulungan ◽  
Effendy DLP. ◽  
Siti MS.
Keyword(s):  
Spectrophotometric Determination ◽  
Ratio Method ◽  
Tablet Form ◽  
Separation Stage ◽  
International Conference ◽  
Absorption Ratio ◽  
Ich Guidelines ◽  
Drug Compound

The Absorption Ratio method involves measuring the absorbance at two wavelengths, namely the iso-absorptive point and the maximum wavelength. Its method could be an option in determining the level of a drug. The aim of this study was to determine whether the absorption ratio method can be used to determine the levels of paracetamol (PCT), propyphenazone (PRO) and caffeine (CAF) in tablet form. The absorption ratio method was used to determine the levels of the mixed drug compound without the separation stage and using the maximum wavelength and iso-absorptif point. The result of the study showed that the absorption ratio method used to solve multicomponent problems in tablet form can be performed and satisfy the validation requirements of the method according to international Conference on Harmonization Q2 (R1) (ICH) guidelines. The absorption ratio method was a simple and accurate to be used determine PCT, PRO, CAF in tablet form. Keywords : Paracetamol, propyphenazone, caffeine, absorption ratio

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