scholarly journals Mechanistic studies on the biosorption of Pb(II) by Pseudomonas aeruginosa

2018 ◽  
Vol 78 (2) ◽  
pp. 290-300
Author(s):  
S. Vimalnath ◽  
H. Ravishankar ◽  
C. Schwandt ◽  
R. V. Kumar ◽  
S. Subramanian

Abstract The biosorption of Pb(II) ions from aqueous solution has been studied using both the intact and thermolyzed cells of Pseudomonas aeruginosa. Further, the role of the major cell wall components, namely DNA, protein, polysaccharide, and lipid, in Pb(II) binding has been assessed using an enzymatic treatment method. The Pb(II) bioremediation capability of P. aeruginosa cells has been investigated by varying the parameters of pH, time of interaction, amount of biomass, and concentration of Pb(II). The complete bioremoval of Pb(II) using intact cells has been achieved for an initial Pb(II) concentration of 12.4 mg L−1 at pH 6.2 and temperature 29 ± 1 °C. The biosorption isotherm follows Langmuirian behavior with a Gibbs free energy of −30.7 kJ mol−1, indicative of chemisorption. The biosorption kinetics is consistent with a pseudo-second-order model. The possible Pb(II) binding mechanisms of P. aeruginosa cells are discussed based on characterization using zeta potential measurements, Fourier transform infra-red spectroscopy, and energy dispersive X-ray spectroscopy. The results confirm that among the major cell wall components studied, polysaccharide shows the highest contribution towards Pb(II) binding, followed by DNA, lipid, and protein. Similar studies using thermolyzed cells show higher Pb(II) uptake compared to the intact cells both before and after enzymatic treatment.

1993 ◽  
Vol 44 (3) ◽  
pp. 405 ◽  
Author(s):  
G Annison

It has been well established over a number of years that the apparent metabolisable energy (AME) value of wheat is highly variable. In 1983 and 1987 in Australia two surveys indicated that approximately 25% of wheats have AME values lower than 13 MJ/kg.DM (range 10.4-15.9 MJ/kg.DM). Following recent studies it has been proposed that the soluble non-starch polysaccharide cell-wall components of wheat (mainly arabinoxylan with some G-glucan) have an anti-nitritive activity when wheats are present at high levels in broiler diets and are responsible for the low-AME wheat phenomenon. The main findings supporting this hypothesis are (1) wheat AME values are negatively correlated with soluble non-starch polysaccharide levels, (2) low level addition (30g/kg) of commercially available pur non-starch polysaccharides to broiler diets depresses the AME,of the diets, (3) degradation of the cell wall polysaccharides in situ by addition of glycanases to broiler diets raises AME values, and (4) addition of purified wheat arabinoxylan to broiler diets depresses the AME in a dose-dependant manner. The AME depression is a result of the inhibition of starch, lipid and proteindigestion in the fore-gut. This paper reviews the experiments and the data from the studies and discusses further aspects of the anti-nutritive activity of cereal polysaccharides in broiler diets. The possible role of the gut microflora in the growth depression observed when diets containing high levels of rye, barley and wheat are fed to broiler chickens is also examined.


Biomolecules ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 36 ◽  
Author(s):  
Andrea Osete-Alcaraz ◽  
Ana Belén Bautista-Ortín ◽  
Encarna Gómez-Plaza

The interactions between tannins and soluble and insoluble cell wall components are, in part, responsible for the low quantities of tannins found in wines compared with the quantities in grapes. The use of polysaccharides to compete with cell wall components could be an interesting approach for improving the chromatic and sensory characteristics of wines. The effect of two commercial polysaccharides, pectin and mannan, on limiting tannin-cell wall interactions was studied in a model solution, measuring the concentration of tannins and polysaccharides remaining in solution after the different interactions by chromatography. The treatment was also tested in a small-scale vinification. Soluble polysaccharides were added to the must and the wines were evaluated at the end of alcoholic fermentation and after six months in the bottle. In the model solution, the commercial polysaccharides formed soluble complexes with the tannins and limited the interactions with cell wall components, with some differences between skin and seed tannins. In the case of the wines, the treatments resulted in wines with a higher color intensity and phenolic content. Sensory analysis resulted in higher scores for the wines with added polysaccharides, since the complexation of tannins with the polysaccharides increased the roundness and body of the resulting wines.


2019 ◽  
Vol 5 (2) ◽  
pp. 34 ◽  
Author(s):  
Fourie ◽  
Pohl

There are many examples of the interaction between prokaryotes and eukaryotes. One such example is the polymicrobial colonization/infection by the various opportunistic pathogenic yeasts belonging to the genus Candida and the ubiquitous bacterium, Pseudomonas aeruginosa. Although this interaction has simplistically been characterized as antagonistic to the yeast, this review highlights the complexity of the interaction with various factors influencing both microbes. The first section deals with the interactions in vitro, looking specifically at the role of cell wall components, quorum sensing molecules, phenazines, fatty acid metabolites and competition for iron in the interaction. The second part of this review places all these interactions in the context of various infection or colonization sites, i.e., lungs, wounds, and the gastrointestinal tract. Here we see that the role of the host, as well as the methodology used to establish co-infection, are important factors, influencing the outcome of the disease. Suggested future perspectives for the study of this interaction include determining the influence of newly identified participants of the QS network of P. aeruginosa, oxylipin production by both species, as well as the genetic and phenotypic plasticity of these microbes, on the interaction and outcome of co-infection.


2016 ◽  
Vol 85 ◽  
pp. 93-108 ◽  
Author(s):  
J. Acera Fernández ◽  
N. Le Moigne ◽  
A.S. Caro-Bretelle ◽  
R. El Hage ◽  
A. Le Duc ◽  
...  

1992 ◽  
Vol 100 (3) ◽  
pp. 1318-1325 ◽  
Author(s):  
Anil P. Ranwala ◽  
Chiyuki Suematsu ◽  
Hiroshi Masuda

1993 ◽  
Vol 44 (3) ◽  
pp. 405 ◽  
Author(s):  
G Annison

It has been well established over a number of years that the apparent metabolisable energy (AME) value of wheat is highly variable. In 1983 and 1987 in Australia two surveys indicated that approximately 25% of wheats have AME values lower than 13 MJ/kg.DM (range 10.4-15.9 MJ/kg.DM). Following recent studies it has been proposed that the soluble non-starch polysaccharide cell-wall components of wheat (mainly arabinoxylan with some G-glucan) have an anti-nitritive activity when wheats are present at high levels in broiler diets and are responsible for the low-AME wheat phenomenon. The main findings supporting this hypothesis are (1) wheat AME values are negatively correlated with soluble non-starch polysaccharide levels, (2) low level addition (30g/kg) of commercially available pur non-starch polysaccharides to broiler diets depresses the AME,of the diets, (3) degradation of the cell wall polysaccharides in situ by addition of glycanases to broiler diets raises AME values, and (4) addition of purified wheat arabinoxylan to broiler diets depresses the AME in a dose-dependant manner. The AME depression is a result of the inhibition of starch, lipid and proteindigestion in the fore-gut. This paper reviews the experiments and the data from the studies and discusses further aspects of the anti-nutritive activity of cereal polysaccharides in broiler diets. The possible role of the gut microflora in the growth depression observed when diets containing high levels of rye, barley and wheat are fed to broiler chickens is also examined.


Crop Science ◽  
2004 ◽  
Vol 44 (5) ◽  
pp. 1546-1552 ◽  
Author(s):  
Silverio García-Lara ◽  
David J. Bergvinson ◽  
Andrew J. Burt ◽  
Al I. Ramputh ◽  
David M. Díaz-Pontones ◽  
...  

2020 ◽  
Vol 19 (4) ◽  
pp. 462-472 ◽  
Author(s):  
Sajad Hussain ◽  
Ting Liu ◽  
Nasir Iqbal ◽  
Marian Brestic ◽  
Ting Pang ◽  
...  

To the best of our knowledge, for the first time our research examines a role of secondary cell wall components in lodging resistance and their interaction with yield under intercropping.


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