Raman microprobe investigation of molecular structure and organization in the native state of woody tissue

FTIR and Raman microanalysis may be used as a powerful combination to determine the identity, and hence infer the source, of contaminant particles which diminish yields of semiconductor components and devices. The complimentarity of these techniques arises from the underlying spectroscopic selection rules.Vibrational spectroscopic techniques are commonly used to characterize the molecular structure of bulk organic materials. These bulk materials typically represent purified fractions of components which may be further investigated with various classical instrumental techniques such as Differential Scanning Calorimetry (DSC), Nuclear Magnetic Resonance (NMR) spectroscopy, UV-Vis spectroscopy. However, these classical technique may have limited value for the interrogation of small impure particles or materials of limited quantity(ng.).Elemental techniques such as Scanning Electron Microscopy coupled to Energy Dispersive Spectroscopy are enhanced by the specificity of FTIR Microprobe Spectroscopy and Raman Microprobe Spectroscopy which are now used in process laboratories to characterize and identify particulate and thin film residues with the intent of device yield enhancement.

Using Viscoelastic Properties of the Woody Tissue from Tobacco Plants (Nicotiana tabacum) to Comment on the Molecular Structure of Cell Walls

Annals of Botany ◽

10.1006/anbo.1998.0619 ◽

1998 ◽

Vol 81 (6) ◽

pp. 729-734 ◽

Cited By ~ 13

Author(s):

D HEPWORTH

Keyword(s):

Molecular Structure ◽

Nicotiana Tabacum ◽

Viscoelastic Properties ◽

Cell Walls ◽

Woody Tissue ◽

Tobacco Plants

Raman Microprobe Evidence for Lignin Orientation in the Cell Walls of Native Woody Tissue

Science ◽

10.1126/science.227.4687.636 ◽

1985 ◽

Vol 227 (4687) ◽

pp. 636-638 ◽

Cited By ~ 159

Author(s):

R. H. ATALLA ◽

U. P. AGARWAL

Keyword(s):

Cell Walls ◽

Woody Tissue ◽

Raman Microprobe

Molecular Structure of the Outermost Cell Wall Component of Spirillum Serpens

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079218 ◽

1977 ◽

Vol 35 ◽

pp. 342-343

Author(s):

Wah Chiu ◽

David Grano

Keyword(s):

Molecular Structure ◽

Cell Wall ◽

Outer Membrane ◽

Gel Electrophoresis ◽

Electron Microscopic Examination ◽

Electron Microscopic ◽

Cell Wall Component ◽

Protein Components ◽

Exposure Technique ◽

Structural Aspects

The periodic structure external to the outer membrane of Spirillum serpens VHA has been isolated by similar procedures to those used by Buckmire and Murray (1). From SDS gel electrophoresis, we have found that the isolated fragments contain several protein components, and that the crystalline structure is composed of a glycoprotein component with a molecular weight of ∽ 140,000 daltons (2). Under an electron microscopic examination, we have visualized the hexagonally-packed glycoprotein subunits, as well as the bilayer profile of the outer membrane. In this paper, we will discuss some structural aspects of the crystalline glycoproteins, based on computer-reconstructed images of the external cell wall fragments.The specimens were prepared for electron microscopy in two ways: negatively stained with 1% PTA, and maintained in a frozen-hydrated state (3). The micrographs were taken with a JEM-100B electron microscope with a field emission gun. The minimum exposure technique was essential for imaging the frozen- hydrated specimens.

Existence of DNA in yeast microbodies

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100082029 ◽

1978 ◽

Vol 36 (2) ◽

pp. 232-233

Author(s):

Masako Osumi ◽

Misuzu Nagano ◽

Hiroko Kazama

Keyword(s):

Catalase Activity ◽

Buoyant Density ◽

Model System ◽

Contour Length ◽

Native State ◽

Normal Alkane ◽

Remarkable Increase ◽

Dna Molecule ◽

Mitochondrial Dnas

We have found that microbodies appeared profusely together with a remarkable increase in catalase activity in normal alkane-grown cells of hydrocarbon-utilizing Candida yeasts, and that the microbodies multiplied by division in these cells. These features of Candida yeasts seem to provide a useful model system for studies on the biogenesis of the microbody. Subsequently, we have succeeded in isolation of Candida microbodies in an apparently native state, as judged biochemically and morphologically. The presence of DNA in the purified microbody fraction thus obtained was proved by the diphenylamine method. DNA molecule of about 15 urn in contour length was released from an isolated microbody. The physicochemical analyses of the microbody DNA revealed that its buoyant density differed from nuclear and mitochondrial DNAs. All these results lead us to the possibility that there is a novel type of DNA in microbodies.