scholarly journals Membrane protein identification by hydrophobicity analysis

2001 ◽  
Author(s):  
Yuval Kluger

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Renxiang Yan ◽  
Xiaofeng Wang ◽  
Lanqing Huang ◽  
Feidi Yan ◽  
Xiaoyu Xue ◽  
...  


Author(s):  
Weizhong Lu ◽  
Jiawei Shen ◽  
Yuqing Qian ◽  
Hongjie Wu ◽  
Yijie Ding ◽  
...  


2018 ◽  
Vol 90 (4) ◽  
pp. 2434-2439 ◽  
Author(s):  
Yanting Zhou ◽  
Jing Gao ◽  
Hongwen Zhu ◽  
Jingjing Xu ◽  
Han He ◽  
...  


1992 ◽  
Vol 1105 (1) ◽  
pp. 141-147 ◽  
Author(s):  
Ansamma Joseph ◽  
Madhu Bhargava ◽  
Eliot Rosen ◽  
Itzhak D. Goldberg


Cells ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 423 ◽  
Author(s):  
Sarah Joost ◽  
Stefan Mikkat ◽  
Michael Wille ◽  
Antje Schümann ◽  
Oliver Schmitt

Acute brain slices are a sample format for electrophysiology, disease modeling, and organotypic cultures. Proteome analyses based on mass spectrometric measurements are seldom used on acute slices, although they offer high-content protein analyses and explorative approaches. In neuroscience, membrane proteins are of special interest for proteome-based analysis as they are necessary for metabolic, electrical, and signaling functions, including myelin maintenance and regeneration. A previously published protocol for the enrichment of plasma membrane proteins based on aqueous two-phase polymer systems followed by mass spectrometric protein identification was adjusted to the small sample size of single acute murine slices from newborn animals and the reproducibility of the results was analyzed. For this, plasma membrane proteins of 12 acute slice samples from six animals were enriched and analyzed by liquid chromatography-mass spectrometry. A total of 1161 proteins were identified, of which 369 were assigned to membranes. Protein abundances showed high reproducibility between samples. The plasma membrane protein separation protocol can be applied to single acute slices despite the low sample size and offers a high yield of identifiable proteins. This is not only the prerequisite for proteome analysis of organotypic slice cultures but also allows for the analysis of small-sized isolated brain regions at the proteome level.



2010 ◽  
Vol 407 (2) ◽  
pp. 196-204 ◽  
Author(s):  
Rui Cao ◽  
Yisong Liu ◽  
Ping Chen ◽  
Rong Lv ◽  
Qin Song ◽  
...  


2008 ◽  
Vol 7 (2) ◽  
pp. 659-665 ◽  
Author(s):  
Maria Jansson ◽  
Kristofer Wårell ◽  
Fredrik Levander ◽  
Peter James


Author(s):  
Sandra Pankow ◽  
Sandra Pankow ◽  
Casimir Bamberger ◽  
Diego Calzolari ◽  
Andreas Bamberger ◽  
...  


PROTEOMICS ◽  
2004 ◽  
Vol 4 (10) ◽  
pp. 3141-3155 ◽  
Author(s):  
Holly D. Coughenour ◽  
Reggie S. Spaulding ◽  
Charles M. Thompson


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