scholarly journals Towards a More Complete Picture: Dissimilatory Metal Reduction by Anaeromyxobacter Species

2004 ◽  
Author(s):  
Frank E. Loeffler
Keyword(s):  
Complete Picture ◽  
Metal Reduction ◽  
Dissimilatory Metal Reduction

scholarly journals A kinetic approach to the dependence of dissimilatory metal reduction byShewanella oneidensisMR-1 on the outer membrane cytochromescOmcA and OmcB

FEBS Journal ◽  
2007 ◽  
Vol 274 (14) ◽  
pp. 3728-3738 ◽  
Author(s):  
Jimmy Borloo ◽  
Bjorn Vergauwen ◽  
Lina De Smet ◽  
Ann Brigé ◽  
Bart Motte ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Kinetic Approach ◽  
Metal Reduction ◽  
Dissimilatory Metal Reduction

scholarly journals Dissimilatory Metal Reduction by the Facultative Anaerobe Pantoea agglomerans SP1

2000 ◽  
Vol 66 (2) ◽  
pp. 543-548 ◽  
Author(s):  
Chris A. Francis ◽  
Anna Y. Obraztsova ◽  
Bradley M. Tebo
Keyword(s):  
Electron Acceptors ◽  
Metal Reduction ◽  
Acetate Oxidation ◽  
Facultative Anaerobe ◽  
16S Rrna Analysis ◽  
Terminal Electron Acceptor ◽  
Coastal Marine ◽  
The Family ◽  
Marine Basin ◽  
Dissimilatory Metal Reduction

ABSTRACT Anaerobic enrichments with acetate as the electron donor and Fe(III) as the terminal electron acceptor were obtained from sediments of Salt Pond, a coastal marine basin near Woods Hole, Mass. A pure culture of a facultatively anaerobic Fe(III) reducer was isolated, and 16S rRNA analysis demonstrated that this organism was most closely related to Pantoea (formerly Enterobacter)agglomerans, a member of the familyEnterobacteriaceae within the gamma subdivision of theProteobacteria. This organism, designated strain SP1, can grow by coupling the oxidation of acetate or H2 to the reduction of a variety of electron acceptors, including Fe(III), Mn(IV), Cr(VI), and the humic substance analog 2,6-anthraquinone disulfonate, but not sulfate. To our knowledge, this is the first mesophilic facultative anaerobe reported to couple acetate oxidation to dissimilatory metal reduction.

scholarly journals Microorganisms Associated with Uranium Bioremediation in a High-Salinity Subsurface Sediment

2003 ◽  
Vol 69 (6) ◽  
pp. 3672-3675 ◽  
Author(s):  
Kelly P. Nevin ◽  
Kevin T. Finneran ◽  
Derek R. Lovley
Keyword(s):  
High Salinity ◽  
Metal Reduction ◽  
Contaminated Aquifer ◽  
Subsurface Sediment ◽  
The Family ◽  
Aquifer Sediments ◽  
Stimulation Of ◽  
Dissimilatory Metal Reduction

ABSTRACT Although stimulation of dissimilatory metal reduction to promote the reductive precipitation of uranium has been shown to successfully remove uranium from some aquifer sediments, the organisms in the family Geobacteraceae that have been found to be associated with metal reduction in previous studies are not known to grow at the high salinities found in some uranium-contaminated groundwaters. Studies with a highly saline uranium-contaminated aquifer sediment demonstrated that the addition of acetate could stimulate the removal of U(VI) from the groundwater. This removal was associated with an enrichment in microorganisms most closely related to Pseudomonas and Desulfosporosinus species.

scholarly journals Molecular Analysis of theIn SituGrowth Rates of Subsurface Geobacter Species

2012 ◽  
Vol 79 (5) ◽  
pp. 1646-1653 ◽  
Author(s):  
Dawn E. Holmes ◽  
Ludovic Giloteaux ◽  
Melissa Barlett ◽  
Milind A. Chavan ◽  
Jessica A. Smith ◽  
...  
Keyword(s):  
Growth Rates ◽  
Ribosomal Proteins ◽  
Specific Growth ◽  
Expression Patterns ◽  
Transcript Abundance ◽  
Metal Reduction ◽  
Content Type ◽  
Specific Growth Rates ◽  
Dissimilatory Metal Reduction

ABSTRACTMolecular tools that can provide an estimate of thein situgrowth rate ofGeobacterspecies could improve understanding of dissimilatory metal reduction in a diversity of environments. Whole-genome microarray analyses of a subsurface isolate ofGeobacter uraniireducens, grown under a variety of conditions, identified a number of genes that are differentially expressed at different specific growth rates. Expression of two genes encoding ribosomal proteins,rpsCandrplL, was further evaluated with quantitative reverse transcription-PCR (qRT-PCR) in cells with doubling times ranging from 6.56 h to 89.28 h. Transcript abundance ofrpsCcorrelated best (r2= 0.90) with specific growth rates. Therefore, expression patterns ofrpsCwere used to estimate specific growth rates ofGeobacterspecies during anin situuranium bioremediation field experiment in which acetate was added to the groundwater to promote dissimilatory metal reduction. Initially, increased availability of acetate in the groundwater resulted in higher expression ofGeobacter rpsC, and the increase in the number ofGeobactercells estimated with fluorescentin situhybridization compared well with specific growth rates estimated from levels ofin situ rpsCexpression. However, in later phases, cell number increases were substantially lower than predicted fromrpsCtranscript abundance. This change coincided with a bloom of protozoa and increased attachment ofGeobacterspecies to solid phases. These results suggest that monitoringrpsCexpression may better reflect the actual rate thatGeobacterspecies are metabolizing and growing duringin situuranium bioremediation than changes in cell abundance.

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