A Glucuronic Acid Binding Leguminous Lectin with Mitogenic Activity Toward Mouse Splenocytes

A lectin designated asHericium erinaceumagglutinin (HEA) was isolated from dried fruiting bodies of the mushroomHericium erinaceumwith a chromatographic procedure which entailed DEAE-cellulose, CM-cellulose, Q-Sepharose, and FPLC Superdex 75. Its molecular mass was estimated to be 51 kDa and its N-terminal amino acid sequences was distinctly different from those of other isolated mushroom lectins. The hemagglutinating activity of HEA was inhibited at the minimum concentration of 12.5 mM by inulin. The lectin was stable at pH 1.9–12.1 and at temperatures up to C, but was inhibited by , , and ions. The lectin exhibited potent mitogenic activity toward mouse splenocytes, and demonstrated antiproliferative activity toward hepatoma (HepG2) and breast cancer (MCF7) cells with an of 56.1 M and 76.5 M, respectively. It manifested HIV-1 reverse transcriptase inhibitory activity with an of 31.7 M. The lectin exhibited potent mitogenic activity toward murine splenocytes but was devoid of antifungal activity.

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Specific Adsorption of Immunoglobulin G1in Bovine Colostrum on Sephadex and Its Mitogenic Activity on Mouse Splenocytes

Agricultural and Biological Chemistry ◽

10.1080/00021369.1991.10860106 ◽

1991 ◽

Vol 55 (12) ◽

pp. 3103-3105

Author(s):

Makoto Miura ◽

Tadao Saito ◽

Haruki Kitazawa ◽

Takatoshi Itoh ◽

Tamio Inamoto

Keyword(s):

Bovine Colostrum ◽

Specific Adsorption ◽

Mitogenic Activity ◽

Mouse Splenocytes

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Characterization of the mitogenic activity elicited by Neisseria gonorrhoeae ribosomal fractions

Canadian Journal of Microbiology ◽

10.1139/m78-094 ◽

1978 ◽

Vol 24 (5) ◽

pp. 579-585

Author(s):

Bernard R. Brodeur

Keyword(s):

Neisseria Gonorrhoeae ◽

Rabbit Antiserum ◽

Mitogenic Activity ◽

Lymphoproliferative Response ◽

Murine Splenocytes ◽

Mouse Splenocytes ◽

Escherichia Coli Cells ◽

Protein Moiety

Ribosomal preparations from Neisseria gonorrhoeae types 1 and 4 were examined for their in vitro stimulation of mouse splenocytes to determine the ribosomal moiety or contaminant responsible for the immunoproliferative activity.In immunodiffusion tests with homologous rabbit antiserum, crude 70S ribosomes formed four precipitin bands while the purified 30S and 50S subunits showed one major line. The same antiserum reacted with lysed N. gonorrhoeae and Neisseria meningitidis A cells but no precipitation occurred with Escherichia coli cells or purified N. gonorrhoeae lipopolysaccharide (LPS). No membrane or LPS contaminant was detected in the purified 30S and 50S preparations. All the ribosomal preparations from virulent and non-virulent N. gonorrhoeae consistently stimulated the murine splenocytes. The mitogenic activity of the 30S and 50S ribosomal preparation was destroyed by treatment with trypsin but only slightly decreased by ribonuclease. It is suggested that the lymphoproliferative response elicited by gonococcal ribosomes is triggered by the protein moiety of the 30S or 50S subunits.

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Mitogenic Activity of Mulberry Lectin (Morus alba L.) on Human Peripheral Blood Lymphocytes

International Conference on Plant, Marine and Environmental Sciences (PMES-2015) Jan. 1-2, 2015 Kuala Lumpur (Malaysia) ◽

10.15242/iicbe.c0115057 ◽

2015 ◽

Keyword(s):

Peripheral Blood ◽

Human Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

Morus Alba ◽

Mitogenic Activity ◽

Blood Lymphocytes ◽

Human Peripheral Blood Lymphocytes

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CHROMATOGRAPHIC STUDIES ON 131I-COMPOUNDS IN FAECES AND BILE

Acta Endocrinologica ◽

10.1530/acta.0.0470343 ◽

1964 ◽

Vol 47 (2) ◽

pp. 343-352

Author(s):

B. Blomstedt ◽

H. Y. Neujahr

Keyword(s):

Intravenous Administration ◽

Glucuronic Acid ◽

Free Triiodothyronine ◽

Considerable Part ◽

Human Faeces ◽

Total Excretion

ABSTRACT The occurrence of 131I-compounds in human faeces was studied after intravenous administration of 131I-thyroxine. The excretion of the radioactivity during 12 days ranged from 7.8 to 32.1 per cent of the dose administered with a mean of 16.0 (± 1.76). The radioactivity in the faeces was derived from free 131I-thyroxine. The total amount of faeces did not seem to influence the total excretion of the dose administered. After administration of 131I-triiodothyronine to rats and humans, triiodothyronine occurred in the bile mainly conjugated with glucuronic acid, but a considerable part of the radioactivity was found in diiodotyrosine. The radioacivity of human faeces after the administration of 131I-triiodothyronine was derived from free triiodothyronine. After injection of 131I-diiodotyrosine only labelled iodide was detected in the bile of rats. In the bile of humans most of the radioactivity was derived from iodide, and a small part of it was found in diiodotyrosine.

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