Influence of Carbohydrate Residues on Antibacterial Activity of Vancomycin

2020 ◽  
Vol 17 (4) ◽  
pp. 287-293
Author(s):  
Justyna Samaszko-Fiertek ◽  
Monika Szulc ◽  
Barbara Dmochowska ◽  
Maciej Jaśkiewicz ◽  
Wojciech Kamysz ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Antibacterial Activity ◽  
Bacterial Cell ◽  
Bacterial Cell Wall ◽  
Reductive Alkylation ◽  
Cell Wall Peptidoglycan ◽  
Mechanism Of Interaction ◽  
Reference Strains

This paper presents synthesis of vancomycin derivatives modified with selected 1- and 2-aminoalditols to carboxylic function and 2,5-anhydro-D-mannose and D-talose to amino function of vancosamine via reductive alkylation. MIC and MBC of these derivatives were determined for reference strains of bacteria: Staphylococcus aureus ATCC 25923, ATCC 6538, ATCC 6538/P, S. epidemidis ATCC 14490, E. faecium PCM 1859, E. faecalis PCM 2673, S. pyogenes PCM 465, and S. pneumonia ATCC 49619 and compared with the activity of vancomycin and its aglycone. Our findings confirm that sugar fragments can play an important role in the mechanism of interaction of vancomycin with bacterial cell wall peptidoglycan.

scholarly journals Rhodomyrtone Accumulates in Bacterial Cell Wall and Cell Membrane and Inhibits the Synthesis of Multiple Cellular Macromolecules in Epidemic Methicillin-Resistant Staphylococcus aureus

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 543
Author(s):  
Ozioma F. Nwabor ◽  
Sukanlaya Leejae ◽  
Supayang P. Voravuthikunchai
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Cell Membrane ◽  
Bacterial Cell ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Treatment Options ◽  
Bacterial Cell Wall ◽  
Methicillin Resistant ◽  
Gram Positive Bacteria ◽  
Inhibitor Compounds

As the burden of antibacterial resistance worsens and treatment options become narrower, rhodomyrtone—a novel natural antibiotic agent with a new antibacterial mechanism—could replace existing antibiotics for the treatment of infections caused by multi-drug resistant Gram-positive bacteria. In this study, rhodomyrtone was detected within the cell by means of an easy an inexpensive method. The antibacterial effects of rhodomyrtone were investigated on epidemic methicillin-resistant Staphylococcus aureus. Thin-layer chromatography demonstrated the entrapment and accumulation of rhodomyrtone within the bacterial cell wall and cell membrane. The incorporation of radiolabelled precursors revealed that rhodomyrtone inhibited the synthesis of macromolecules including DNA, RNA, proteins, the cell wall, and lipids. Following the treatment with rhodomyrtone at MIC (0.5–1 µg/mL), the synthesis of all macromolecules was significantly inhibited (p ≤ 0.05) after 4 h. Inhibition of macromolecule synthesis was demonstrated after 30 min at a higher concentration of rhodomyrtone (4× MIC), comparable to standard inhibitor compounds. In contrast, rhodomyrtone did not affect lipase activity in staphylococci—both epidemic methicillin-resistant S. aureus and S. aureus ATCC 29213. Interfering with the synthesis of multiple macromolecules is thought to be one of the antibacterial mechanisms of rhodomyrtone.

Targeting Bacterial Cell Wall Peptidoglycan Synthesis by Inhibition of Glycosyltransferase Activity

2015 ◽  
Vol 87 (2) ◽  
pp. 190-199 ◽  
Author(s):  
Michael F. Mesleh ◽  
Premraj Rajaratnam ◽  
Mary Conrad ◽  
Vasu Chandrasekaran ◽  
Christopher M. Liu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacterial Cell ◽  
Bacterial Cell Wall ◽  
Peptidoglycan Synthesis ◽  
Cell Wall Peptidoglycan

scholarly journals Three-dimensional structure of the bacterial cell wall peptidoglycan

2006 ◽  
Vol 103 (12) ◽  
pp. 4404-4409 ◽  
Author(s):  
S. O. Meroueh ◽  
K. Z. Bencze ◽  
D. Hesek ◽  
M. Lee ◽  
J. F. Fisher ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacterial Cell ◽  
Three Dimensional ◽  
Bacterial Cell Wall ◽  
Dimensional Structure ◽  
Three Dimensional Structure ◽  
Cell Wall Peptidoglycan

The Site of Inhibition of Bacterial Cell Wall Peptidoglycan Synthesis by Azureomycin B, a New Antibiotic1

1980 ◽  
Vol 88 (2) ◽  
pp. 565-570 ◽  
Author(s):  
Priska SPIRI-NAKAGAWA ◽  
Ruiko ÖIWA ◽  
Yoshitake TANAKA ◽  
Haruo TANAKA ◽  
Satoshi ÖMURA
Keyword(s):  
Cell Wall ◽  
Bacterial Cell ◽  
Bacterial Cell Wall ◽  
Peptidoglycan Synthesis ◽  
Cell Wall Peptidoglycan

Design and evaluation of analogues of the bacterial cell-wall peptidoglycan motif l-Lys-d-Ala-d-Ala for use in a vancomycin biosensor

2007 ◽  
Vol 17 (21) ◽  
pp. 5758-5762 ◽  
Author(s):  
Olivier Hernout ◽  
Karine Berthoin ◽  
Isabelle Delattre ◽  
Paul M. Tulkens ◽  
Stéphane Carryn ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacterial Cell ◽  
Bacterial Cell Wall ◽  
Cell Wall Peptidoglycan

scholarly journals Characterization of Colicin M and its Orthologs Targeting Bacterial Cell Wall Peptidoglycan Biosynthesis

2012 ◽  
Vol 18 (3) ◽  
pp. 222-229 ◽  
Author(s):  
Hélène Barreteau ◽  
Meriem El Ghachi ◽  
Aurélie Barnéoud-Arnoulet ◽  
Emmanuelle Sacco ◽  
Thierry Touzé ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacterial Cell ◽  
Bacterial Cell Wall ◽  
Peptidoglycan Biosynthesis ◽  
Cell Wall Peptidoglycan ◽  
Colicin M
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