Recent Advances of Useful Cell Sources in the Periodontal Regeneration

2019 ◽  
Vol 14 (1) ◽  
pp. 3-8 ◽  
Author(s):  
Young-Dan Cho ◽  
Kyoung-Hwa Kim ◽  
Hyun-Mo Ryoo ◽  
Yong-Moo Lee ◽  
Young Ku ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Tissue Regeneration ◽  
Periodontal Regeneration ◽  
Periodontal Tissue ◽  
Therapy Outcomes ◽  
Differentiated Cells ◽  
Periodontal Tissue Regeneration ◽  
Cell Sources ◽  
Induced Pluripotent

Background: Periodontitis is an inflammatory disease that can result in destruction of the tooth attachment apparatus. Therefore, periodontal tissue regeneration is currently an important focus of research in the field. Approaches using stem cells and reprogrammed cells, such as induced pluripotent stem cells (iPSCs) or trans-differentiated cells, represent the cutting edge in periodontal regeneration, and have led to many trials for their clinical application. Objectives and Results: In this review, we consider all available stem cell sources, methods to obtain the cells, their capability to differentiate into the desired cells, and the extent of their utilization in periodontal regeneration. In addition, we introduce the new concepts of using iPSCs and transdifferentiated cells for periodontal regeneration. Finally, we discuss the promise of tissue engineering for improving cell therapy outcomes for periodontal regeneration. Conclusions: Despite their limitations, iPSCs and trans-differentiated cells may be promising cell sources for periodontal tissue regeneration. Further collaborative investigation is required for the effective and safe application of these cells in combination with tissue engineering elements, like scaffolds and biosignals.

Periodontal Tissue Regeneration with Adipose-Derived Stem Cells

2007 ◽  
pp. 110306233438005 ◽  
Author(s):  
Morikuni Tobita ◽  
A. Cagri Uysal ◽  
Rei Ogawa ◽  
Hiko Hyakusoku ◽  
Hiroshi Mizuno
Keyword(s):  
Stem Cells ◽  
Tissue Regeneration ◽  
Adipose Derived Stem Cells ◽  
Periodontal Tissue ◽  
Periodontal Tissue Regeneration

scholarly journals Local Injection of Allogeneic Stem Cells from Apical Papilla Enhanced Periodontal Tissue Regeneration in Minipig Model of Periodontitis

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Guoqing Li ◽  
Nannan Han ◽  
Xiuli Zhang ◽  
Haoqing Yang ◽  
Yangyang Cao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tissue Regeneration ◽  
Alveolar Bone ◽  
Local Injection ◽  
Swine Model ◽  
Periodontal Tissue ◽  
Clinical Assessments ◽  
Periodontal Tissues ◽  
Periodontal Tissue Regeneration ◽  
Apical Papilla

Background. Discovering suitable seeding cells and simple application technique will be beneficial for MSC-mediated treatment of periodontitis. Stem cells from apical papilla (SCAPs) might be the candidate seeding cell for the periodontal tissues regeneration based on their origin and characters. In this research, we investigated the effect of SCAPs on periodontal tissue regeneration in swine by local injection. Methods. We established experimental periodontitis model in miniature pigs and then treated them with SCAPs by local injection. Clinical assessments, computed tomography (CT) scanning, histologic examination, and quantitative measurements were used to evaluate the effect of periodontal tissues regeneration. Results. At 12 weeks after injection, clinical assessments showed that probing depth, gingival recession, and attachment loss values were 5.44±0.77 mm versus 7.33±1.0 mm (p<0.01), 2.33±0.33 mm versus 2.11±0.69 mm (p>0.05), and 7.78±0.84 mm versus 9.44±1.07 mm (p<0.01) in SCAPs group and 0.9% NaCl group, respectively. CT scan results showed a significant increase of 12.86 mm3 alveolar bone regeneration in SCAPs group compared with 0.9% NaCl group. In addition, histopathology results demonstrated remarkable regeneration in SCAPs group, whereas regeneration of periodontal tissue was hardly found in 0.9% NaCl group. Conclusion. Local injection of SCAPs could effectively restore tissue defects brought about by periodontitis in the swine model. Thus, SCAPs, as an easily accessible dental-deriving stem cell, may serve as an alternative application for periodontitis treatment.

Experimental Formation of Periodontal Structure Around Titanium Implants Utilizing Bone Marrow Mesenchymal Stem Cells: A Pilot Study

2009 ◽  
Vol 35 (3) ◽  
pp. 106-129 ◽  
Author(s):  
Mona K. Marei ◽  
Manal M. Saad ◽  
Adham M. El-Ashwah ◽  
Rania M. El-Backly ◽  
Mohammed A. Al-Khodary
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Dental Implants ◽  
Tissue Regeneration ◽  
Titanium Implants ◽  
Autogenous Bone ◽  
Periodontal Tissue ◽  
Periodontal Tissue Regeneration ◽  
Experimental Side

Abstract Tissue engineering in the head and neck area, presents numerous advantages. One of the most remarkable advantages is that regeneration of only a small amount of tissue can be highly beneficial to the patient, particularly in the field of periodontal tissue regeneration. For decades, successful osseointegration has provided thousands of restorations that maintain normal function. With the increasing need to utilize dental implants for growing patients and enhance their function to simulate normal tooth physiology and proprioception, there appears to be an urgent need for the concept of periodontal tissue regeneration around dental implants. In the present work, 5 goats were used for immediate implant placement post canine teeth extraction. Each goat received 2 implant fixtures; the control side received a porous hollow root-form poly (DL-Lactide-co-Glycolide) scaffold around the titanium fixture, and the experimental side received the same scaffold but seeded with autogenous bone marrow–derived mesenchymal stem cells. One animal was killed 10 days postoperatively, and the others were killed after 1 month. The results showed that on the experimental side, periodontal-like tissue with newly formed bone was demonstrated both at 10 days and after 1 month, while the control specimens showed early signs of connective tissue regeneration around the titanium fixture at 10 days, but was not shown in the 1 month specimens. It can be concluded that undifferentiated mesenchymal stem cells were capable of differentiating to provide the 3 critical tissues required for periodontal tissue regeneration: cementum, bone, and periodontal ligament. This work may provide a new approach for periodontal tissue regeneration.

scholarly journals Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Lin Liu ◽  
Kun Liu ◽  
Yanzhe Yan ◽  
Zhuangzhuang Chu ◽  
Yi Tang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Tissue Regeneration ◽  
Periodontal Ligament ◽  
Periodontal Tissue ◽  
Alizarin Red ◽  
Differentiation Potential ◽  
Alp Activity ◽  
Periodontal Tissue Regeneration

Objectives. Enhanced migration and osteogenic differentiation of mesenchymal stem cells (MSCs) are beneficial for MSC-mediated periodontal tissue regeneration, a promising method for periodontitis treatment. FBXO5, a member of the F-box protein family, is involved in the osteogenic differentiation of MSCs. Here, we investigated the effect of FBXO5 on human periodontal ligament stem cells (hPDLSCs). Materials and Methods. hPDLSCs were isolated from periodontal ligament tissue. Lentivirus FBXO5 shRNA was used to silence FBXO5 expression. Two transcripts of FBXO5 were overexpressed and transduced into hPDLSCs via retroviral infection. Migration and osteogenic differentiation of hPDLSCs were evaluated using the scratch migration assay, alkaline phosphatase (ALP) activity, ALP staining, alizarin red staining, western blotting, and real-time polymerase chain reaction. Results. The expression of FBXO5 was upregulated after osteogenic induction in hPDLSCs. FBXO5 knockdown attenuated migration, inhibited ALP activity and mineralization, and decreased RUNX2, OSX, and OCN expression, while the overexpression of two transcript isoforms significantly accelerated migration, enhanced ALP activity and mineralization, and increased RUNX2, OSX, and OCN expression in hPDLSCs. Conclusions. Both isoforms of FBXO5 promoted the migration and osteogenic differentiation potential of hPDLSCs, which identified a potential target for improving periodontal tissue regeneration.

scholarly journals Application of induced pluripotent stem (iPS) cells in periodontal tissue regeneration

2010 ◽  
Vol 226 (1) ◽  
pp. 150-157 ◽  
Author(s):  
Xuejing Duan ◽  
Qisheng Tu ◽  
Jin Zhang ◽  
Jinhai Ye ◽  
Cesar Sommer ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Ips Cells ◽  
Periodontal Tissue ◽  
Periodontal Tissue Regeneration ◽  
Induced Pluripotent

scholarly journals The Effect of Diabetes Mellitus on IGF Axis and Stem Cell Mediated Regeneration of the Periodontium

2021 ◽  
Vol 8 (12) ◽  
pp. 202
Author(s):  
Nancy M. S. Hussein ◽  
Josie L. Meade ◽  
Hemant Pandit ◽  
Elena Jones ◽  
Reem El-Gendy
Keyword(s):  
Diabetes Mellitus ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Diabetic Patients ◽  
Periodontal Tissue ◽  
Signalling Molecules ◽  
Periodontal Tissues

Periodontitis and diabetes mellitus (DM) are two of the most common and challenging health problems worldwide and they affect each other mutually and adversely. Current periodontal therapies have unpredictable outcome in diabetic patients. Periodontal tissue engineering is a challenging but promising approach that aims at restoring periodontal tissues using one or all of the following: stem cells, signalling molecules and scaffolds. Mesenchymal stem cells (MSCs) and insulin-like growth factor (IGF) represent ideal examples of stem cells and signalling molecules. This review outlines the most recent updates in characterizing MSCs isolated from diabetics to fully understand why diabetics are more prone to periodontitis that theoretically reflect the impaired regenerative capabilities of their native stem cells. This characterisation is of utmost importance to enhance autologous stem cells based tissue regeneration in diabetic patients using both MSCs and members of IGF axis.

scholarly journals Maintained Properties of Aged Dental Pulp Stem Cells for Superior Periodontal Tissue Regeneration

2019 ◽  
Vol 10 (4) ◽  
pp. 793 ◽  
Author(s):  
Linsha Ma ◽  
Jingchao Hu ◽  
Yu Cao ◽  
Yilin Xie ◽  
Hua Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tissue Regeneration ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Periodontal Tissue ◽  
Periodontal Tissue Regeneration
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