scholarly journals Mast Cells and Angiogenesis in Oral Malignant and Premalignant Lesions

2008 ◽  
Vol 2 (1) ◽  
pp. 126-132 ◽  
Author(s):  
E.Z Michailidou ◽  
A.K Markopoulos ◽  
D.Z Antoniades
Keyword(s):  
Squamous Cell Carcinoma ◽  
Mast Cell ◽  
Mast Cells ◽  
Cell Carcinoma ◽  
Oral Mucosa ◽  
Squamous Cell ◽  
Premalignant Lesions ◽  
Angiogenic Switch ◽  
Normal Oral Mucosa ◽  
Human Cd34

Mast cell contribution to neoangiogenesis during tumorigenesis in oral squamous cell carcinoma is not determined yet. Objectives: To associate numerical mast cell density (MCD) to numerical microvessel density (MVD) during the progression of oral leukoplakia without dysplasia and leukoplakia with dysplasia to squamous cell carcinoma (OSCC). Materials and methods: MVD was analysed immunohistochemically (mouse monoclonal anti-human CD34) in 49 paraffin-embedded specimens, 35 OSCCs, 9 leukoplakias and 5 normal oral tissues. Toluidine blue counterstaining revealed mast cells. MCD and MVD were assessed at the same optical field. Results: MVD increased between: normal oral mucosa, dysplasia (p=0.004), OSCC (p=0.001), leukoplakia and OSCC (p=0.041). MCD increased between: normal oral mucosa, dysplasia (p=0.003), OSCC (p=0.000), leukoplakia and OSCC (p=0.007). MVD was found to depend on MCD (p=0.000) in a percent 28.3% (power curve fit model). Conclusions: Mast cells are attracted at the lesion site and may turn on an angiogenic switch during tumorigenesis in OSCC.

scholarly journals Comparative analysis of the mast cell density in normal oral mucosa, actinic cheilitis and lip squamous cell carcinoma

2008 ◽  
Vol 19 (3) ◽  
pp. 186-189 ◽  
Author(s):  
Ana Paula Neutzling Gomes ◽  
Julia Elis Johann ◽  
Gabriela Gularte Lovato ◽  
Aline Marques Ferreira
Keyword(s):  
Squamous Cell Carcinoma ◽  
Mast Cells ◽  
Cell Carcinoma ◽  
Oral Mucosa ◽  
Squamous Cell ◽  
Severe Dysplasia ◽  
Histochemical Technique ◽  
Actinic Cheilitis ◽  
Normal Oral Mucosa ◽  
Mast Cell Density

Previous studies have shown that the number of mast cells is increased in ultraviolet (UV) irradiated skin and in neoplasias. Actinic cheilitis (AC) is a lesion caused by excessive exposure to sunlight that can transform into lip squamous cell carcinoma. The aim of this study was to compare the number of mast cells in 4 groups: NOM = normal oral mucosa (n=6); MDAC = mild dysplasia in actinic cheilitis (n=13); SDAC = severe dysplasia in actinic cheilitis (n=13); and LSCC = lip squamous cell carcinoma (n=15). The sections were stained by histochemical technique of blue toluidine and visual counting was performed with the aid of a reticulum coupled to the microscope ocular. A calibrated observer performed the count in 5 fields by case at ×400 magnification. The largest mean number of mast cells per group was observed in LSCC (40.1), followed by MDAC (30.5), SDAC (28.6) and NOM (12.2). There were significant differences between NOM and MDAC (p<0.05) and between NOM and LSCC (p<0.05). The increased density of mast cells observed in AC and in LSCC compared to NOM suggests a role for the mast cells in the development of these lesions.

scholarly journals Correlation of Mast Cell and Angiogenesis in Oral Lichen Planus, Dysplasia (Leukoplakia), and Oral Squamous Cell Carcinoma

2021 ◽  
Vol 12 (2) ◽  
pp. e0016
Author(s):  
Amutha Sundararajan ◽  
◽  
Rajmohan Muthusamy ◽  
Kumar Gopal Siva ◽  
Prasad Harikrishnan ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Mast Cell ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Lichen Planus ◽  
Oral Mucosa ◽  
Squamous Cell ◽  
Oral Lichen Planus ◽  
Normal Oral Mucosa ◽  
Oral Lichen

Objective: The aim of this study was to compare and correlate mast cell density (MCD) and microvessel density (MVD) between normal oral mucosa, oral lichen planus, various grades of dysplasia, and oral squamous cell carcinoma (OSCC). Materials and Methods: The study comprised a total of 75 samples, of which 65 were archival tissue blocks of histopathologically confirmed cases, which included 10 cases of oral lichen planus, 25 cases of dysplasia (mild [n=10], moderate [n=10], and severe [n=5]), and 30 cases of OSCC (well differentiated [n=10], moderately differentiated [n=10], and poorly differentiated [n=10]), and 10 samples of normal oral mucosa. All the sections were immunohistochemically stained with anti-CD34 and counterstained with toluidine blue stain. Mean MCD and MVD were determined and analyzed using ANOVA test and compared between the lesions using Tukey HSD test. Pearson’s correlation coefficient test was used to correlate these two factors between various lesions. Results: Mean MCD and mean MVD were found to be increased in all the lesions compared to normal oral mucosa, and the values were statically significant. Overall, MCD and MVD showed a significant positive correlation (r=0.640). Conclusion: Increase of MCD and MVD and their positive correlation in all the lesions have emphasized their role in the pathogenesis and disease progression.

scholarly journals p53 immunohistochemical staining patterns in oral squamous cell carcinoma

2016 ◽  
Vol 6 (12) ◽  
pp. 1013-1017
Author(s):  
G Dundy ◽  
H Kumar ◽  
A Singh ◽  
A Chandarakant
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Oral Mucosa ◽  
Squamous Cell ◽  
P53 Protein ◽  
P Value ◽  
P53 Expression ◽  
Normal Oral Mucosa ◽  
Significant Difference

Background: Mutation of p53 gene is one of the most common events in oral carcinogenesis. Accumulation of p53 protein has also been detected in premalignant lesions.Materials and Methods:  This study included 40 biopsy samples, which were received in department of pathology, Sarojini Naidu Medical College, Agra, to ascertain p53 expression by immunohistochemically, in patients with oral squamous cell carcinomas and to correlate its expression with histological grade, different sites in oral cavity and tobacco intake/smoking habits.Results: Out of 40 biopsies of oral mucosa, 03 showed normal oral mucosa and 37 were diagnosed as squamous cell carcinoma (SCC), most patients were in 5th and 6th decade and majority (86.5%) of oral SCC were males with buccal mucosa being the most common site. There was a statistically significant difference in p53 expression between oral SCC and normal oral mucosa (p value <0.05). Of total 37 cases, 12 cases were well differentiated type, 16 moderately differentiated and 09 of poorly differentiated type of SCC. In each category, about two thirds were positive for p53 staining. Out of total 37 cases of oral SCC, 64.9% were positive and 35.1% were negative for p53 expression, 34 cases had positive history of tobacco intake/smoking habits, of which 23 cases were positive while 11 cases were negative for p53 staining.Conclusion: Abnormal p53 protein was detected in 64.9% of oral squamous cell carcinoma, but not in normal oral mucosa. p53 expression was associated with malignant transformation of oral mucosa. 

scholarly journals Comparison of Immunohistochemical Expression of Antiapoptotic Protein Survivin in Normal Oral Mucosa, Oral Leukoplakia, and Oral Squamous Cell Carcinoma

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Amita Negi ◽  
Abhiney Puri ◽  
Rakhi Gupta ◽  
Rajat Nangia ◽  
Alisha Sachdeva ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Oral Mucosa ◽  
Squamous Cell ◽  
Oral Leukoplakia ◽  
Immunohistochemical Expression ◽  
Antiapoptotic Protein ◽  
Normal Oral Mucosa ◽  
Survivin Protein

Background. Oral squamous cell carcinoma is the sixth most frequent malignant tumor worldwide and the third most common cancers in developing countries. Oral leukoplakia is the best-known precursor lesion of oral squamous cell carcinoma. The aim of the present study was to compare immunohistochemical expression of antiapoptotic protein survivin in normal oral mucosa, oral leukoplakia, and oral squamous cell carcinoma. Method. Total 45 specimens of formalin fixed paraffin embedded tissue blocks, 15 in each of the following: normal oral mucosa, leukoplakia, and oral squamous cell carcinoma were used for the study. Immunohistochemical reaction for survivin protein was performed for the 4 µm thick histological sections taken on positively charged slides. Results. 20% normal mucosa cases, 53.33% cases of leukoplakia, and 80% of oral squamous cell carcinoma were found out to be survivin positive. One way ANOVA test indicated statistically significant difference of survivin expression between the three different groups p<0.001. Conclusion. A high incidence of survivin protein expression in oral epithelial dysplasia and squamous cell carcinoma samples indicate that survivin protein expression may be an early event in initiation and progression of oral squamous cell carcinoma.

Prevalence of DNA of the different types of oncogenic viruses in the squamous cell carcinoma and normal oral mucosa

2020 ◽  
Vol 25 (3) ◽  
pp. 206-210
Author(s):  
S. I. Kutukova ◽  
A. B. Chukhlovin ◽  
A. I. Yaremenko ◽  
Y. V. Ivaskova ◽  
A. Y. Razumova ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Oral Mucosa ◽  
Squamous Cell ◽  
Oncogenic Viruses ◽  
Normal Oral Mucosa ◽  
Different Types
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