Rapid Determination of Cellulose, Neutral Sugars, and Uronic Acids from Plant Cell Walls by One-step Two-step Hydrolysis and HPAEC-PAD

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (20) ◽  
Author(s):  
Trevor Yeats ◽  
Tamara Vellosillo ◽  
Nadav Sorek ◽  
Ana Ibáñez ◽  
Stefan Bauer
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Rapid Determination ◽  
Plant Cell Walls ◽  
Uronic Acids ◽  
Neutral Sugars ◽  
One Step

Determination of phenolic acids in plant cell walls by microwave digestion

1994 ◽  
Vol 64 (1) ◽  
pp. 63-65 ◽  
Author(s):  
Gordon J Provan ◽  
Lorraine Scobbie ◽  
Andrew Chesson
Keyword(s):  
Plant Cell ◽  
Phenolic Acids ◽  
Cell Walls ◽  
Microwave Digestion ◽  
Plant Cell Walls

scholarly journals Determination of Subunit Composition of Clostridium cellulovorans Cellulosomes That Degrade Plant Cell Walls

2002 ◽  
Vol 68 (4) ◽  
pp. 1610-1615 ◽  
Author(s):  
Koichiro Murashima ◽  
Akihiko Kosugi ◽  
Roy H. Doi
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Wall ◽  
Scaffolding Protein ◽  
Plant Cell Walls ◽  
Higher Plant ◽  
Clostridium Cellulovorans ◽  
Plant Cell Wall Degradation

ABSTRACT Clostridium cellulovorans produces a cellulase enzyme complex (cellulosome). In this study, we isolated two plant cell wall-degrading cellulosomal fractions from culture supernatant of C. cellulovorans and determined their subunit compositions and enzymatic activities. One of the cellulosomal fractions showed fourfold-higher plant cell wall-degrading activity than the other. Both cellulosomal fractions contained the same nine subunits (the scaffolding protein CbpA, endoglucanases EngE and EngK, cellobiohydrolase ExgS, xylanase XynA, mannanase ManA, and three unknown proteins), although the relative amounts of the subunits differed. Since only cellobiose was released from plant cell walls by the cellulosomal fractions, cellobiohydrolases were considered to be key enzymes for plant cell wall degradation.

scholarly journals Growth of Neocallimastix sp. Strain R1 on Italian Ryegrass Hay: Removal of Neutral Sugars from Plant Cell Walls

1989 ◽  
Vol 55 (6) ◽  
pp. 1363-1367 ◽  
Author(s):  
Michael K. Theodorou ◽  
Annette C. Longland ◽  
Mewa S. Dhanoa ◽  
Susan E. Lowe ◽  
Anthony P. J. Trinci
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Italian Ryegrass ◽  
Plant Cell Walls ◽  
Neutral Sugars ◽  
Ryegrass Hay

Rapid and simple determination of O-acetyl groups bound to plant cell walls by acid hydrolysis and 1H NMR measurement

1994 ◽  
Vol 35 (4) ◽  
pp. 959-961 ◽  
Author(s):  
Iiyama Kenji ◽  
Bach Tuyet Lam Thi ◽  
Kasuya Natsuki ◽  
Bruce A. Stone
Keyword(s):  
Acid Hydrolysis ◽  
1H Nmr ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Walls
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