Determination of the Uronic Acid Content of Plant Cell Walls Using a Colorimetric Assay

2001 ◽  
Vol 00 (1) ◽  
Author(s):  
Laurence D. Melton ◽  
Bronwen G. Smith
Keyword(s):  
Plant Cell ◽  
Acid Content ◽  
Cell Walls ◽  
Uronic Acid ◽  
Colorimetric Assay ◽  
Plant Cell Walls ◽  
Uronic Acid Content

Determination of phenolic acids in plant cell walls by microwave digestion

1994 ◽  
Vol 64 (1) ◽  
pp. 63-65 ◽  
Author(s):  
Gordon J Provan ◽  
Lorraine Scobbie ◽  
Andrew Chesson
Keyword(s):  
Plant Cell ◽  
Phenolic Acids ◽  
Cell Walls ◽  
Microwave Digestion ◽  
Plant Cell Walls

scholarly journals Molecular cohesion in plant cell walls. Methylation analysis of pectic polysaccharides from the cotyledons of white mustard

1969 ◽  
Vol 115 (3) ◽  
pp. 431-439 ◽  
Author(s):  
D. A. Rees ◽  
N. J. Wight
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Uronic Acid ◽  
Structural Elements ◽  
Plant Cell Walls ◽  
White Mustard ◽  
Methylation Analysis ◽  
Structural Units ◽  
Pectic Polysaccharides ◽  
Derivatives Of

Methylation analysis was used to characterize the pectic polysaccharides from mustard cotyledons, a tissue with potential for rapid biological change involving the walls. The methylated sugars were identified by g.l.c. and paper chromatography after conversion of uronic acid derivatives into [3H]hexoses, and confirmed by the formation of crystalline derivatives of most of the main products, which were: 2,3-di-O-methyl-d-[6−3H]galactose, 2-O-methyl-d-[6−3H]galactose, 3,4-di-O-methylrhamnose, 3-O-methylrhamnose, 2,3,5-tri-O-methyl-l-arabinose, 2,3-di-O-methyl-l-arabinose, 2-O-methyl-l-arabinose, 2,3,4-tri-O-methyl-d-xylose and 2,3,4,6-tetra-O-methyl-d-galactose in the molar proportions 1·00:1·14:0·54:0·74:2·86:2·50:2·24:1·88:0·32. The structural units present are similar to those in wellknown polysaccharides from mature tissues, but their proportions are strikingly different. Uninterrupted and unbranched galacturonan segments can therefore contribute little cohesion to these walls, and it is suggested that this correlates with a function of the wall matrix to hydrate and permit readjustment, during germination, of structural elements or wall surfaces or both.

Determination of aldoses and uronic acid content of vegetable fiber

1979 ◽  
Vol 96 (2) ◽  
pp. 282-292 ◽  
Author(s):  
Robert R. Selvendran ◽  
John F. March ◽  
Stephen G. Ring
Keyword(s):  
Acid Content ◽  
Uronic Acid ◽  
Uronic Acid Content ◽  
Vegetable Fiber

scholarly journals Determination of Subunit Composition of Clostridium cellulovorans Cellulosomes That Degrade Plant Cell Walls

2002 ◽  
Vol 68 (4) ◽  
pp. 1610-1615 ◽  
Author(s):  
Koichiro Murashima ◽  
Akihiko Kosugi ◽  
Roy H. Doi
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Wall ◽  
Scaffolding Protein ◽  
Plant Cell Walls ◽  
Higher Plant ◽  
Clostridium Cellulovorans ◽  
Plant Cell Wall Degradation

ABSTRACT Clostridium cellulovorans produces a cellulase enzyme complex (cellulosome). In this study, we isolated two plant cell wall-degrading cellulosomal fractions from culture supernatant of C. cellulovorans and determined their subunit compositions and enzymatic activities. One of the cellulosomal fractions showed fourfold-higher plant cell wall-degrading activity than the other. Both cellulosomal fractions contained the same nine subunits (the scaffolding protein CbpA, endoglucanases EngE and EngK, cellobiohydrolase ExgS, xylanase XynA, mannanase ManA, and three unknown proteins), although the relative amounts of the subunits differed. Since only cellobiose was released from plant cell walls by the cellulosomal fractions, cellobiohydrolases were considered to be key enzymes for plant cell wall degradation.

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