scholarly journals Compatibility of Lemongrass Extract with Spodoptera litura Nuclear Polyhedrosis Virus Against Spodoptera litura on Soybean Plants

2021 ◽  
Vol 2 (1) ◽  
pp. 7-13
Author(s):  
Nafisatul Afidah ◽  
Tutung Hadiastono ◽  
Bedjo Bedjo ◽  
Fery Abdul Choliq
Keyword(s):  
Spodoptera Litura ◽  
Biocontrol Agent ◽  
Nuclear Polyhedrosis Virus ◽  
Randomized Design ◽  
Important Pest ◽  
Completely Randomized Design ◽  
Polyhedrosis Virus ◽  
Soybean Plants ◽  
Nuclear Polyhedrosis ◽  
Tuber Crops

Armyworm (Spodoptera litura) is an important pest that damages the leaves of soybean. SlNPV is one type of pathogen that has potential as a biocontrol agent to control armyworms because it is specific, selective, and effective. This study aimed to investigate the compatibility between the lemongrass and SlNPV in controlling armyworm. The Research was conducted at the Laboratory of Indonesian Legumes and Tuber Crops Research Institute (ILETRI) from February 2016 until April 2016. The study using completely randomized design with 7 treatments and 3 replication with a treatment that consists of control, SlNPV-JTM 97C 1,5 × 106 PIB/ml, 10% of lemongrass extract, a combination SlNPV-JTM 97C fixed concentrate of 1,5 × 106 PIB/ml with 2.5%, 5%, 7.5%, and 10% of lemongrass extract. The results showed that the combination of SlNPV-JTM 97C 1.5 × 106 PIB/ml with 10% lemongrass had the highest level of virulence, with 96% of mortality on larva 100% on the pupa stage, so that the combination of SlNPV and lemongrass extracts was compatible in controlling S.litura.

scholarly journals Uji Efektivitas Konsentrasi Spodoptera litura – Nuclear Polyhedrosis Virus (SlNPV) JTM 97C Formulasi Bubuk Terhadap Larva Spodoptera litura Fabricius (Lepidoptera: Noctuidae) Pada Tanaman Kedelai

2019 ◽  
Vol 2 (2) ◽  
pp. 46
Author(s):  
M Novel Ghufron Syahroni ◽  
Nanang Tri Haryadi
Keyword(s):  
Spodoptera Litura ◽  
Nuclear Polyhedrosis Virus ◽  
Larval Instar ◽  
Crop Failure ◽  
Randomized Design ◽  
Pathogenic Virus ◽  
Hot And Humid Climates ◽  
Polyhedrosis Virus ◽  
Soybean Plants ◽  
Nuclear Polyhedrosis

Spodoptera litura (armyworm) is an important leaf pest that is polyphagic and has a broad range of hosts. Spodoptera litura is the main pest in soybean plants and can cause up to 50% damage and often results in decreased productivity and even crop failure. Spodoptera litura is widespread in areas with hot and humid climates from the subtropics to the tropics. S. litura pests attack cultivation plants in the vegetative and generative phases. The prospect of insect pathogens as a substitute for chemical insecticides is quite good in terms of their effectiveness and impact on the environment. Nuclear Polyhedrosis Virus (NPV) is one of the Baculoviridae viruses that infect the larval stage. Spodoptera litura Nuclear Polyhedrosis Virus (SlNPV) is a pathogenic virus that infects armyworms. SlNPV JTM 97C is an effective isolate in controlling larvae of Spodoptera litura. The larvae used in this study were larval instar 4, instar 5, and instar 6. The study was conducted using a Completely Randomized Design (CRD) with 6 treatments and 3 replications. The treatments consisted of controls, 1 g / l, 2g / l, 3 g / l, 4 g / l, and 5 g / l. Each treatment was applied to instar 4, instar 5 and 6 instar larvae. The results showed that SlNPV JTM 97C affected mortality, stopped eating and pupa formation of instar 4, instar 5 larvae, but did not affect instar 6 larvae. The younger the larval instar the higher the pathogenicity response.

scholarly journals THE POTENTIAL OF SUBCULTURE Helicoverpa armigera NUCLEAR POLYHEDROSIS VIRUS (HaNPV) TO BE UTILIZED AS AN ALTERNATE SYNTHETIC INSECTICIDES TO CONTROL INSECTS PESTS IN CABBAGES PLANTATION (Brassica oleracea var. capitata L)

2015 ◽  
Vol 2 (1) ◽  
pp. 405
Author(s):  
Mia Miranti ◽  
Wawan Hermawan ◽  
Melanie . ◽  
Rahman Perdana Hadi ◽  
Dicky Budi Sugiarto ◽  
...  
Keyword(s):  
Helicoverpa Armigera ◽  
Spodoptera Litura ◽  
Nuclear Polyhedrosis Virus ◽  
Viral Agent ◽  
Alternate Host ◽  
Randomized Design ◽  
Helicoverpa Armígera ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Entomopathogenic Viruses

<p>Subculture Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV) is an entomopathogenic viruses isolated from cadaver of Helicoverpa armigera larvae, and was succesfully produced in Spodoptera litura larvae as an alternate host. These viral agent have been applied to control the population of three species insects pests Spodoptera litura, Crocidolomia pavonana and Plutella xyllostella which were infested in Cabbages Plantation. The concentration of virus of 4 x 107 Polyhedral/ml was sprayed in cabbage leaves 24 hours before infested of larval and every four days after. The research used randomized design which consisted of factor (three species of second instar larvae, S. litura, C. pavonana, P. xyllostella) and eight replications. The result showed that the three species of larval were sensitive against subculture HaNPV infection. The mortality of each larval species (S. litura, C. pavonana and P. xyllostella) were 100%, 97.5% and 98.7%. Subculture HaNPV can be utilized to control the population of three spesies of insects pests in Cabbage Plantation. </p><p>Keywords : Subculture HaNPV, Polyhedral</p>

Movement of Nuclear Polyhedrosis Virus into Velvetbean Caterpillar (Lepidoptera: Noctuidae) Larval Populations on Soybean by Nabis roseipennis (Heteroptera: Nabidae) Nymphs

1992 ◽  
Vol 27 (2) ◽  
pp. 126-134 ◽  
Author(s):  
S. Y. Young ◽  
W. C. Yearian
Keyword(s):  
Nuclear Polyhedrosis Virus ◽  
Larval Mortality ◽  
Anticarsia Gemmatalis ◽  
Velvetbean Caterpillar ◽  
Nabis Roseipennis ◽  
Polyhedrosis Virus ◽  
Soybean Plants ◽  
Nuclear Polyhedrosis ◽  
Mixed Age ◽  
Lepidoptera Noctuidae

Nabis roseipennis Reuter nymphs that preyed on larvae of the nuclear polyhedrosis virus (NPV) infected velvetbean caterpillar, Anticarsia gemmatalis (Hübner), excreted the virus (AgNPV) for several days thereafter. Based on bioassays, fifth instar and second instar nymphs excreted 84.7 × 105 and 9.7 × 105 polyhedral inclusion bodies (PIB) per nymph, respectively. The AgNPV-contaminated nymphs effectively disseminated the virus via the feces over soybean plants where it served as inoculum to initiate disease in larval populations of A. gemmatalis caged in the field. Larval mortality from AgNPV ranged from 11.4 to 48.5% over treatments in two tests. Larval mortality in treatments where the source of virus inoculum was AgNPV-contaminated fifth instar nymphs was similar to that in treatments where the source of viral inoculum was diseased larvae. Larval mortality resulting from AgNPV dissemination by the nymphs was usually higher in treatments containing fifth instar nymphs than in those with second instar nymphs. Dissemination of NPV by fifth instar nymphs was higher in mixed-age than in uniformed-age A. gemmatalis larval populations. This was not the case with the smaller second instar nymphs.

scholarly journals The Role of Cytochrome c on Apoptosis Induced by Anagrapha falcifera Multiple Nuclear Polyhedrosis Virus in Insect Spodoptera litura Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e40877 ◽  
Author(s):  
Kaiyu Liu ◽  
Duanyang Shu ◽  
Na Song ◽  
Zhongchao Gai ◽  
Yuan Yuan ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Spodoptera Litura ◽  
Nuclear Polyhedrosis Virus ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Anagrapha Falcifera

EFFECTS OF LARVAL AGE ON SUSCEPTIBILITY OF SPODOPTERA LITURA (LEPIDOPTERA: NOCTUIDAE) TO SPODOPTERA LITURA MULTIPLE NUCLEAR POLYHEDROSIS VIRUS

2000 ◽  
Vol 132 (3) ◽  
pp. 337-340 ◽  
Author(s):  
M.D. Monobrullah ◽  
Masao Nagata
Keyword(s):  
Spodoptera Litura ◽  
Nuclear Polyhedrosis Virus ◽  
Actual Mechanism ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Time Required ◽  
Lepidoptera Noctuidae ◽  
Host Development

Susceptibility of many insects to baculoviruses decreases with host development (Hochberg 1991), whereas the time required for infected hosts to die increases with larval age (Boucias et al. 1980; Bucher and Turnock 1983; Smits and Vlak 1988). The actual mechanism of this phenomenon, however, has not been established in many cases. The present study was undertaken to investigate the effect of larval age on the susceptibility of Spodoptera litura Fabricius to nuclear polyhedrosis virus (NPV) through oral and intrahaemocoelic routes of infection.

scholarly journals Effectiveness of Nuclear Polyhedrosis Virus and Bacillus thuringiensis alone and in Combination against Spodoptera litura (Fabricius)

2019 ◽  
Vol 51 (2) ◽  
Author(s):  
Sumaira Maqsood ◽  
Muhammad Afzal ◽  
Muhammad Saleem Haider ◽  
Hafiz Azhar Ali Khan ◽  
Muhammad Ali ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Spodoptera Litura ◽  
Nuclear Polyhedrosis Virus ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

scholarly journals Biological Test of Formulation of Subculture Helicovepa armigera Nuclear Polihedrosis Virus (HaNPV) on Mortality of Spodoptera litura Larvae infested to cabbage (Brassica oleracea Var. capitata Linn.) Plantation

2015 ◽  
Vol 2 (1) ◽  
pp. 646
Author(s):  
Desy Efriyani Anggraeny Nasution ◽  
Mia Miranti ◽  
Melanie .
Keyword(s):  
Brassica Oleracea ◽  
Helicoverpa Armigera ◽  
Spodoptera Litura ◽  
Nuclear Polyhedrosis Virus ◽  
Insect Pests ◽  
Block Design ◽  
Economic Value ◽  
Helicoverpa Armígera ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

<p>Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV) is one of the biological agents that could be used for the development of organic farming in Indonesia. One of the organic agricultural food products which have high economic value is cabbage, HaNPV effectif to control the population of several species of insect pests, can be used instead of synthetic insecticides in agricultural development efforts of organic cabbage. HaNPV formulation can be used as a way to maintain the virulence properties of the virus and facilitate the storage and application. The preparation of subculture HaNPV formulations was in liquid, powders, cornstarch and talk carrier material. The design experimental was used randomized block design consists single factor (type HaNPV formulation consisting of powder formulations, liquid formulation, formulation in cornstarch, and talc formulation including water formulation without virus as a control), with eight replications. The results showed that all of the formulations of subculture HaNPV causing larvae mortality at range 97.4% -100%. The highest mortality of larvae occurred in cabbage plants are protected by HaNPV carrier liquid and talk preparations by 100% each. <br /><strong></strong></p><p><strong>Keywords</strong>: Formulations Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV), subculture, Spodoptera litura, Brassica oleracea Var. capitata Linn.</p>

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