scholarly journals High Resolution Microsatellite Marker Analysis of Some Rice Landraces Using Metaphor Agarose Gel Electrophoresis

2016 ◽  
Vol 20 (1) ◽  
pp. 54 ◽  
Author(s):  
K. Kristamtini ◽  
T. Taryono ◽  
Panjisakti Basunanda ◽  
Rudi Hari Murti
Keyword(s):  
Microsatellite Markers ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Molecular Techniques ◽  
Agarose Gel ◽  
Chain Reaction ◽  
Rice Landraces ◽  
Polymorphic Pattern ◽  
Polymerase Chain ◽  
Microsatellite Marker Analysis

Microsatellite markers or simple sequences repeats are DNA - based molecular techniques that areused to see the different among accessions and inbred lines. There are three methods to analysis the results ofthe polymerase chain reaction of microsatellite markers namely polyacrylamide gel electrophoresis (PAGE),capillary electroforesis, and Metaphor Agarose Gel Electroforesis (MAGE), and the Use of MAGE assessedmore easily and economically the polymorphic pattern of DNA markers. This study aimed to obtain fast,effective and efficient in term of easy and cheap technique to identify microsatellite markers of some blackrice cultivars and F2 populations from crosses between black with white rice. The results showed that MAGEsuccessfully separated clearly SSRs alleles with different sizes of less than 25 bp .

scholarly journals ANALYSIS OF THE INHIBITORY POTENTIAL OF STREPTOCOCCUS SALIVARIUS ISOLATED FROM ADULT SALIVA AND THE TONGUE DORSUM FOR THE GROWTH OF CANDIDA ALBICANS

2017 ◽  
Vol 9 ◽  
pp. 3
Author(s):  
Nindy Fairiska ◽  
Boy M Bachtiar ◽  
Ferry P Gultom
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Albicans ◽  
Sodium Dodecyl Sulfate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Streptococcus Salivarius ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Inhibitory Potential

Objectives: The objective of this study is to analyze the effectiveness of Streptococcus salivarius and its protein for inhibiting the growth of Candidaalbicans.Methods: The analysis was conducted using polymerase chain reaction, sodium dodecyl sulfate polyacrylamide gel electrophoresis, a Bradford test,deferred antagonism test, and well-diffusion agar.Result: S. salivarius, isolated from saliva and the tongue dorsum, and its protein do not inhibit the growth of C. albicans. The morphology of C. albicansdid not change after being exposed to protein produced by S. salivarius.Conclusions: S. salivarius and its protein do not inhibit the growth of C. albicans. However, the bacterium has the capacity to maintain fungusmorphology in the form of blastospora.

Molecular Beacon Polymerase Chain Reaction Detection of Escherichia coli O157:H7 in Milk

2000 ◽  
Vol 63 (7) ◽  
pp. 855-859 ◽  
Author(s):  
JOHN L. McKILLIP ◽  
MARYANNE DRAKE
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Gel Electrophoresis ◽  
Molecular Beacon ◽  
Skim Milk ◽  
Agarose Gel ◽  
Escherichia Coli O157 ◽  
Agarose Gel Electrophoresis ◽  
Chain Reaction ◽  
Polymerase Chain

A fluorescently labeled oligonucleotide probe (molecular beacon) was applied to detect Escherichia coli O157:H7 in artificially contaminated skim milk during polymerase chain reaction (PCR) amplification of extracted DNA. The probe was designed to hybridize with a region of the slt-II gene coding for the A subunit and to fluoresce when the hairpin-stem conformation was linearized upon hybridization to the target sequence. The molecular beacon was incorporated into PCR reactions containing DNA extracted from artificially contaminated skim milk. The degree of fluorescence was monitored in PCR reactions containing 103, 105, and 107 CFU of E. coli O157:H7 per ml and was found to correlate with the amount of template in each reaction. Fluorescence significantly increased above background levels by cycle 8, 14, or 14 in reactions containing DNA from the 107-, 105-, or 103-CFU/ml template, respectively (P < 0.05). Molecular beacon PCR demonstrated positive results more rapidly than traditional agarose gel electrophoresis analysis of PCR products. Use of molecular beacons allows real-time monitoring of PCR reactions, and the closed-tube format allows simultaneous detection and confirmation of target amplicons without the need for agarose gel electrophoresis and/or Southern blotting. This is the first report of a stem-and-loop molecular beacon being applied for direct detection of a pathogen in food.

scholarly journals Polyvinylpyrrolidone-Agarose Gel Electrophoresis Purification of Polymerase Chain Reaction-Amplifiable DNA from Soils

1993 ◽  
Vol 59 (6) ◽  
pp. 1972-1974 ◽  
Author(s):  
Charles C. Young ◽  
Robert L. Burghoff ◽  
Lois G. Keim ◽  
Vera Minak-Bernero ◽  
James R. Lute ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Chain Reaction ◽  
Polymerase Chain
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