PREDNISOLONE ENCAPSULATED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES FOR TARGET DRUG DELIVERY – DESIGN AND QUANTIFICATION

Objective: The present study aimed to develop a novel type of superparamagnetic iron oxide nanoparticles (SPIONs) to deliver prednisolone at colon as a target site for the treatment of inflammatory bowel disease (IBD) such as ulcerative colitis and Crohn’s disease which may further progress to cancer. Methods: SPIONs were synthesized using a coprecipitation method. Further, it was encapsulated with prednisolone-polyethylene glycol by double emulsion method (W1/O/W2). The prepared formulations were characterized for its physicochemical characterization such as scanning electron microscopy, X-ray diffraction, particle size and zeta potential, encapsulation efficiency, and in vitro drug release. Results: The results reveal that the physicochemical property of the formulations complies with the standard values and in vitro release of prednisolone in the first 18 h, attains 57 and 58% and it reaches 71 and 75% at 24 h, and this is statistically significant (p˂0.0177). This release result implies that the drug release from the formulations is controllable and sustains manner. Conclusion: Our findings could be a promising approach for the delivery of prednisolone with enhanced half-life for the treatment of IBD through colon targeting.

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Systematic investigations on heating effects of carboxyl-amine functionalized superparamagnetic iron oxide nanoparticles (SPIONs) based ferrofluids for in vitro cancer hyperthermia therapy

Journal of Molecular Liquids ◽

10.1016/j.molliq.2018.02.029 ◽

2018 ◽

Vol 256 ◽

pp. 224-237 ◽

Cited By ~ 26

Author(s):

Ganeshlenin Kandasamy ◽

Atul Sudame ◽

Piyush Bhati ◽

Anindita Chakrabarty ◽

Dipak Maity

Keyword(s):

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Superparamagnetic Iron Oxide ◽

Superparamagnetic Iron Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Amine Functionalized ◽

Heating Effects ◽

Hyperthermia Therapy ◽

Cancer Hyperthermia

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Biodistribution and Toxicity Assessment of Superparamagnetic Iron Oxide Nanoparticles In Vitro and In Vivo

Current Medical Science ◽

10.1007/s11596-018-1989-8 ◽

2018 ◽

Vol 38 (6) ◽

pp. 1096-1102 ◽

Cited By ~ 10

Author(s):

Qin Yu ◽

Xiao-qin Xiong ◽

Lei Zhao ◽

Ting-ting Xu ◽

Hao Bi ◽

...

Keyword(s):

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Superparamagnetic Iron Oxide ◽

Toxicity Assessment ◽

Superparamagnetic Iron Oxide Nanoparticles ◽

Oxide Nanoparticles

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Efficient and Rapid Labeling of Transplanted Cell Populations with Superparamagnetic Iron Oxide Nanoparticles Using Cell Surface Chemical Biotinylation for in Vivo Monitoring by MRI

Cell Transplantation ◽

10.3727/096368910x498250 ◽

2010 ◽

Vol 19 (4) ◽

pp. 419-429 ◽

Cited By ~ 20

Author(s):

Po-Wah So ◽

Tammy Kalber ◽

David Hunt ◽

Michael Farquharson ◽

Alia Al-Ebraheem ◽

...

Keyword(s):

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Cell Tracking ◽

Superparamagnetic Iron Oxide ◽

Superparamagnetic Iron Oxide Nanoparticles ◽

Cell Populations ◽

Oxide Nanoparticles ◽

Signal Loss

Determination of the dynamics of specific cell populations in vivo is essential for the development of cell-based therapies. For cell tracking by magnetic resonance imaging (MRI), cells need to internalize, or be surface labeled with a MRI contrast agent, such as superparamagnetic iron oxide nanoparticles (SPIOs): SPIOs give rise to signal loss by gradient-echo and T2-weighted MRI techniques. In this study, cancer cells were chemically tagged with biotin and then magnetically labeled with anti-biotin SPIOs. No significant detrimental effects on cell viability or death were observed following cell biotinylation. SPIO-labeled cells exhibited signal loss compared to non-SPIO-labeled cells by MRI in vitro. Consistent with the in vitro MRI data, signal attenuation was observed in vivo from SPIO-labeled cells injected into the muscle of the hind legs, or implanted subcutaneously into the flanks of mice, correlating with iron detection by histochemical and X-ray fluorescence (XRF) methods. To further validate this approach, human mesenchymal stem cells (hMSCs) were also employed. Chemical biotinylation and SPIO labeling of hMSCs were confirmed by fluorescence microscopy and flow cytometry. The procedure did not affect proliferation and multipotentiality, or lead to increased cell death. The SPIO-labeled hMSCs were shown to exhibit MRI signal reduction in vitro and was detectable in an in vivo model. In this study, we demonstrate a rapid, robust, and generic methodology that may be a useful and practical adjuvant to existing methods of cell labeling for in vivo monitoring by MRI. Further, we have shown the first application of XRF to provide iron maps to validate MRI data in SPIO-labeled cell tracking studies.

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