Isolation and characterization of Ralstonia solanacearum from infected tomato plants of Bishnupur district of Manipur

Abstract To defend against invading pathogens, plants possess RNA silencing mechanisms involving small RNAs (miRNAs, siRNAs). Also viroids – plant infectious, non-coding, unencapsidated RNA – cause the production of viroid-specific small RNAs (vsRNA), but viroids do escape the cytoplasmic silencing mechanism. Viroids with minor sequence variations can produce different symptoms in infected plants, suggesting an involvement of vsRNAs in symptom production. We analyzed by deep sequencing the spectrum of vsRNAs induced by the PSTVd strain AS1, which causes strong symptoms such as dwarfing and necrosis upon infection of tomato plants cv Rutgers. Indeed, vsRNAs found with highest frequency mapped to the pathogenicity-modulating domain of PSTVd, supporting an involvement of vsRNAs in symptom production. Furthermore, in PSTVd AS1-infected plants the accumulation of some endogenous miRNAs, which are involved in leaf development via regulation of transcription factors, is suppressed. The latter finding supports the hypothesis that a miRNA-dependent (mis)regula-tion of transcription factors causes the viroid symptoms.

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Isolation and Characterization of Ralstonia solanacearum causing Bacterial Wilt of Potato in Konkan Region of Maharashtra

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2020.910.018 ◽

2020 ◽

Vol 9 (10) ◽

pp. 136-142

Author(s):

U. R. Phondekar ◽

R.G. Bhagwat ◽

R.R. Rathod ◽

Amruta D. Gadhave ◽

Y.R. Nirgude ◽

...

Keyword(s):

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Isolation And Characterization

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Isolation and characterization of Pseudomonas brassicacearum J12 as an antagonist against Ralstonia solanacearum and identification of its antimicrobial components

Microbiological Research ◽

10.1016/j.micres.2012.01.003 ◽

2012 ◽

Vol 167 (7) ◽

pp. 388-394 ◽

Cited By ~ 53

Author(s):

Tiantian Zhou ◽

Da Chen ◽

Chunyu Li ◽

Qian Sun ◽

Lingzhi Li ◽

...

Keyword(s):

Ralstonia Solanacearum ◽

Isolation And Characterization ◽

Pseudomonas Brassicacearum

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Isolation and characterization of Ralstonia solanacearum strains causing bacterial wilt of potato in Nakuru County of Kenya

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2018.16659 ◽

2018 ◽

Vol 17 (52) ◽

pp. 1455-1465

Author(s):

Romeo Chamedjeu Rostand ◽

Masanga Joel ◽

Matiru Viviene ◽

Runo Steven

Keyword(s):

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Isolation And Characterization

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Isolation and characterization of bacteriophages that infect Ralstonia solanacearum in Thailand

Acta Horticulturae ◽

10.17660/actahortic.2018.1207.20 ◽

2018 ◽

pp. 155-162 ◽

Cited By ~ 1

Author(s):

A. Bhunchoth ◽

N. Phironrit ◽

C. Leksomboon ◽

T. Kawasaki ◽

T. Yamada ◽

...

Keyword(s):

Ralstonia Solanacearum ◽

Isolation And Characterization

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Isolation and characterization of fluorescent Pseudomonas with bio-control potential against Ralstonia solanacearum

Indian Phytopathology ◽

10.1007/s42360-021-00400-9 ◽

2021 ◽

Author(s):

Thalhun Lhingkhanthem Kipgen ◽

Lohit Chandra Bora ◽

Gunajit Goswami ◽

Madhumita Barooah ◽

Pradip Kumar Borah ◽

...

Keyword(s):

Ralstonia Solanacearum ◽

Control Potential ◽

Fluorescent Pseudomonas ◽

Isolation And Characterization

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Effects of Combined Application of Potassium Silicate and Salicylic Acid on the Defense Response of Hydroponically Grown Tomato Plants to Ralstonia solanacearum Infection

Sustainability ◽

10.3390/su13073750 ◽

2021 ◽

Vol 13 (7) ◽

pp. 3750

Author(s):

Ni-Hao Jiang ◽

Shi-Han Zhang

Keyword(s):

Salicylic Acid ◽

Combination Treatment ◽

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Combined Effects ◽

Combined Treatments ◽

Tomato Plants ◽

Combined Application ◽

Infected Tomato ◽

Tomato Bacterial Wilt

Bacterial wilt, caused by soilborne pathogenic bacterium Ralstonia solanacearum, is a serious and widespread disease that affects global tomato production. Both silicon (Si) and salicylic acid (SA) play important roles in enhancing tomato resistance against bacterial wilt, however, their combined effects on the defense responses of infected tomato plants remain unknown. Hence, the combined effects of Si and SA on physiological and biochemical parameters of R. solanacearum-infected tomato plants were investigated. The combination treatment of Si and SA significantly decreased disease incidences, lipoxygenase (LOX) activity and ethylene (ET) production. The combined treatments were more prominent in improving the morphological traits of root systems, such as root length, root surface area, average root diameter and root volume. The activities of polyphenol oxidase (PPO) and peroxidase (POD) and the concentrations of total soluble phenolics (TSPs) and lignin-thioglycolic acid (LTGA) derivatives were significantly increased in the plants with combined treatments. Si in combination with SA could significantly enhance neutral invertase (NI) and acid invertases (AI) activities in the leaves of tomato plants at 3 days post-infection (dpi) compared with application of Si alone. Three defense-related genes, PAL, POD and pathogenesis-related protein 1 (PR1), were significantly induced in Si+SA treatment at 7 dpi when compared with individual application of Si or SA. The expression level of salicylic acid-binding protein 2 (SABP2) was significantly higher for combination treatment when compared with treatment of Si or SA alone. The possible mechanisms involved in the synergistic effects of Si and SA on the control of tomato bacterial wilt were proposed. This study indicates that under hypertonic conditions, the combined application of 2.0 mM potassium silicate (K2SiO3) and 0.5 mM SA had a synergistic effect on the control of tomato bacterial wilt.

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