scholarly journals Isolation and Characterization of Ralstonia solanacearum Causing Bacterial Wilt of Tomato in Nigeria

2015 ◽  
Vol 29 (1) ◽  
pp. 1 ◽  
Author(s):  
AR Popoola ◽  
SA Ganiyu ◽  
OA Enikuomehin ◽  
JG Bodunde ◽  
OB Adedibu ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Ralstonia solanacearum causing Bacterial Wilt of Potato in Konkan Region of Maharashtra

2020 ◽  
Vol 9 (10) ◽  
pp. 136-142
Author(s):  
U. R. Phondekar ◽  
R.G. Bhagwat ◽  
R.R. Rathod ◽  
Amruta D. Gadhave ◽  
Y.R. Nirgude ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of Ralstonia solanacearum strains causing bacterial wilt of potato in Nakuru County of Kenya

2018 ◽  
Vol 17 (52) ◽  
pp. 1455-1465
Author(s):  
Romeo Chamedjeu Rostand ◽  
Masanga Joel ◽  
Matiru Viviene ◽  
Runo Steven
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Ralstonia solanacearum Causing Bacterial Wilt of Solanaceae Crops

2017 ◽  
Vol 6 (5) ◽  
pp. 1173-1190 ◽  
Author(s):  
Sujeet Kumar ◽  
Kedar nath ◽  
N. Hamsaveni ◽  
P.H. Ramanjini Gowda ◽  
I.B. Rohini ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Isolation And Characterization

scholarly journals Genetic Diversity and Aggressiveness of Ralstonia solanacearum Strains Causing Bacterial Wilt of Potato in Uruguay

Plant Disease ◽  
2011 ◽  
Vol 95 (10) ◽  
pp. 1292-1301 ◽  
Author(s):  
M. I. Siri ◽  
A. Sanabria ◽  
M. J. Pianzzola
Keyword(s):  
Genetic Diversity ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Potato Tubers ◽  
Bacterial Strains ◽  
Potato Plants ◽  
Polymerase Chain ◽  
First Time ◽  
Different Levels

Bacterial wilt, caused by Ralstonia solanacearum, is a major disease affecting potato (Solanum tuberosum) production worldwide. Although local reports suggest that the disease is widespread in Uruguay, characterization of prevalent R. solanacearum strains in that country has not been done. In all, 28 strains of R. solanacearum isolated from major potato-growing areas in Uruguay were evaluated, including 26 strains isolated from potato tubers and 2 from soil samples. All strains belonged to phylotype IIB, sequevar 1 (race 3, biovar 2). Genetic diversity of strains was assessed by repetitive-sequence polymerase chain reaction, which showed that the Uruguayan strains constituted a homogeneous group. In contrast, inoculation of the strains on tomato and potato plants showed, for the first time, different levels of aggressiveness among R. solanacearum strains belonging to phylotype IIB, sequevar 1. Aggressiveness assays were also performed on accessions of S. commersonii, a wild species native to Uruguay that is a source of resistance for potato breeding. No significant interactions were found between bacterial strains and potato and S. commersonii genotypes, and differences in aggressiveness among R. solanacearum strains were consistent with previously identified groups based on tomato and potato inoculations. Moreover, variation in responses to R. solanacearum was observed among the S. commersonii accessions tested.

scholarly journals Characterization of the Interaction Between the Bacterial Wilt Pathogen Ralstonia solanacearum and the Model Legume Plant Medicago truncatula

2007 ◽  
Vol 20 (2) ◽  
pp. 159-167 ◽  
Author(s):  
Fabienne Vailleau ◽  
Elodie Sartorel ◽  
Marie-Françoise Jardinaud ◽  
Fabien Chardon ◽  
Stéphane Genin ◽  
...  
Keyword(s):  
Plant Species ◽  
Medicago Truncatula ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Recombinant Inbred Lines ◽  
Model Legume ◽  
Legume Plant ◽  
Hrp Genes

The soilborne pathogen Ralstonia solanacearum is the causal agent of bacterial wilt and attacks more than 200 plant species, including some legumes and the model legume plant Medicago truncatula. We have demonstrated that M. truncatula accessions Jemalong A17 and F83005.5 are susceptible to R. solanacearum and, by screening 28 R. solana-cearum strains on the two M. truncatula lines, differential interactions were identified. R. solanacearum GMI1000 infected Jemalong A17 line, and disease symptoms were dependent upon functional hrp genes. An in vitro root inoculation method was employed to demonstrate that R. solanacearum colonized M. truncatula via the xylem and intercellular spaces. R. solanacearum multiplication was restricted by a factor greater than 1 × 105 in the resistant line F83005.5 compared with susceptible Jemalong A17. Genetic analysis of recombinant inbred lines from a cross between Jemalong A17 and F83005.5 revealed the presence of major quantitative trait loci for bacterial wilt resistance located on chromosome 5. The results indicate that the root pathosystem for M. truncatula will provide useful traits for molecular analyses of disease and resistance in this model plant species.

scholarly journals Biocontrol potential of bacteriophage ɸsp1 against bacterial wilt-causing Ralstonia solanacearum in Solanaceae crops

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Pramila Devi Umrao ◽  
Vineet Kumar ◽  
Shilpa Deshpande Kaistha
Keyword(s):  
Potato Tuber ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Burst Size ◽  
Field Application ◽  
Bacteriophage Therapy ◽  
Tomato Seedlings ◽  
Isolation And Characterization ◽  
Wide Ph Range ◽  
Ph Range

Abstract Background Bacteriophages are effective biocontrol strategy as well as ecofriendly remedy for the emerging antibiotic and chemical resistance in bacterial phytopathogens such as bacterial wilt-causing Ralstonia solanacearum. One of the major challenges in the use of bacteriophage therapy for agricultural phytopathogens is maintaining their viability even during variations in pH, temperature, ultraviolet irradiation, and desiccation during field application for sustainable agriculture. Results In this study, the isolation and characterization of phage ɸsp1 for its efficacy against wilt-causing R. solanacearum performed on Solanum lycopersicum (tomato) seedlings and Solanum tuberosum (potato) tuber assay are reported. Bacteriophage was found to be viable and stable at a wide pH range (3.0–9.0) and at temperatures up to 55 °C. Phage ɸsp1 required ~15 min for adsorption and completed its life cycle in 25–30 min by host cell lysis with a burst size of ~250–300. Phage ɸsp1 eradicated 94.73% preformed R. solanacearum biofilm and inhibited biofilm formation by 73.68% as determined by the static crystal violet microtiter biofilm assay. Transmission electron microscope revealed the phage ɸsp1 to be approximately 208±15 nm in size, comprising of icosahedral head (100 ±15 nm) and tail, as belonging to Myoviridae family. Plant bioassays showed 81.39 and 87.75% reduction in pathogen count using phages ɸsp1 in potato tuber and tomato seedlings, respectively. Reversal in disease symptoms was 100% in phage-treated tuber and tomato plant (pot assay) compared to only pathogen-treated controls. Conclusion Isolated bacteriophage ɸsp1 was found to be highly host specific, effective in biofilm prevention, and capable of inhibiting bacterial wilt at low multiplicity of infection (1.0 MOI) in tomato as well as potato tuber bioassays. Phages ɸsp1 were environmentally stable as they survive at variable pH and temperature. Bacteriophage ɸsp1 shows a promise for development into a biocontrol formulation for the prevention of R. solanacearum bacterial wilt disease.

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