Biochemical characterization of a sphingomonad isolate from the ascocarp of white truffle (Tuber magnatum Pico)

Available information on bacteria that influence the economically important white truffle (Tuber magnatum Pico) life cycle is scarce. From the ascocarp of white truffle we isolated a strain TMG 022C, capable for growth in nitrogendepleted conditions and assimilation of mannitol and trehalose. According to 16S rDNA sequence phylogeny, the strain was closely related to Sphingobium amiense. The strain had the ability to perform ammonification, reduce nitrate and solubilize Ca3(PO4)2, produce chitinase, lipase, phospholipase and ?-glucanase, but not cellulase, pectinase, protease and siderophores. The results suggest that Sphingobium sp. TMG 022C could have an influence on the Tuber magnatum life cycle through improved mycelium nutrition and ascocarp decomposition.

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Erratum to: Deodorization of pig slurry and characterization of bacterial diversity using 16S rDNA sequence analysis

The Journal of Microbiology ◽

10.1007/s12275-014-0704-0 ◽

2014 ◽

Vol 52 (12) ◽

pp. 1056-1056

Author(s):

Ok-Hwa Hwang ◽

Sebastian Raveendar ◽

Young-Ju Kim ◽

Ji-Hun Kim ◽

Tae-Hun Kim ◽

...

Keyword(s):

Sequence Analysis ◽

Bacterial Diversity ◽

16S Rdna ◽

Rdna Sequence ◽

Pig Slurry ◽

16S Rdna Sequence ◽

16S Rdna Sequence Analysis

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Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.02723-0 ◽

2004 ◽

Vol 54 (1) ◽

pp. 47-57 ◽

Cited By ~ 98

Author(s):

Jeroen Heyrman ◽

Bram Vanparys ◽

Niall A. Logan ◽

An Balcaen ◽

Marina Rodríguez-Díaz ◽

...

Keyword(s):

Sequence Analysis ◽

16S Rdna ◽

Type Strain ◽

Biochemical Characterization ◽

Agricultural Research ◽

Research Area ◽

Fatty Acid Analysis ◽

Rdna Sequence ◽

16S Rdna Sequence ◽

16S Rdna Sequence Analysis

A group of 42 isolates were isolated from the soil of several disused hay fields, in the Drentse A agricultural research area (The Netherlands), that were taken out of production at different times. The group represents hitherto-uncultured Bacillus lineages that have previously been found, by a non-cultural method, to be predominant in soil. The strains were subjected to a polyphasic taxonomic study, including (GTG)5-PCR, 16S rDNA sequence analysis, DNA–DNA hybridizations, DNA base-ratio determination, fatty acid analysis and morphological and biochemical characterization. By comparing the groupings obtained by (GTG)5-PCR and 16S rDNA sequence analysis, six clusters of similar strains could be recognized. A DNA–DNA relatedness study showed that these clusters represented five novel genospecies. Further analysis supported the proposal of five novel species in the genus Bacillus, namely Bacillus novalis sp. nov. (type strain IDA3307T=R-15439T=LMG 21837T=DSM 15603T), Bacillus vireti sp. nov. (type strain IDA3632T=R-15447T=LMG 21834T=DSM 15602T), Bacillus soli sp. nov. (type strain IDA0086T=R-16300T=LMG 21838T=DSM 15604T), Bacillus bataviensis sp. nov. (type strain IDA1115T=R-16315T=LMG 21833T=DSM 15601T) and Bacillus drentensis sp. nov. (type strain IDA1967T=R-16337T=LMG 21831T=DSM 15600T).

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16S rDNA Sequence Based Characterization of Bacteria in Stored Jaggery in Indian Jaggery Manufacturing Units

Sugar Tech ◽

10.1007/s12355-012-0162-0 ◽

2012 ◽

Vol 14 (4) ◽

pp. 422-427 ◽

Cited By ~ 3

Author(s):

A. K. Verma ◽

Shubhra Singh ◽

Shalini Singh ◽

Ashutosh Dubey

Keyword(s):

16S Rdna ◽

Rdna Sequence ◽

16S Rdna Sequence

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Deodorization of pig slurry and characterization of bacterial diversity using 16S rDNA sequence analysis

The Journal of Microbiology ◽

10.1007/s12275-014-4251-5 ◽

2014 ◽

Vol 52 (11) ◽

pp. 918-929 ◽

Cited By ~ 7

Author(s):

Ok-Hwa Hwang ◽

Sebastian Raveendar ◽

Young-Ju Kim ◽

Ji-Hun Kim ◽

Tae-Hun Kim ◽

...

Keyword(s):

Sequence Analysis ◽

Bacterial Diversity ◽

16S Rdna ◽

Rdna Sequence ◽

Pig Slurry ◽

16S Rdna Sequence ◽

16S Rdna Sequence Analysis

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Isolation and characterization of aPseudomonassp. strain PH1 utilizing meta-aminophenol

Canadian Journal of Microbiology ◽

10.1139/w99-132 ◽

2000 ◽

Vol 46 (3) ◽

pp. 211-217 ◽

Cited By ~ 15

Author(s):

Razia Kutty ◽

Hemant J Purohit ◽

Purushottam Khanna

Keyword(s):

Nitrogen Source ◽

16S Rdna ◽

Sole Source ◽

Rdna Sequence ◽

Pseudomonas Sp ◽

16S Rdna Sequence ◽

Industry Waste ◽

Isolation And Characterization ◽

Dye Industry

Pseudomonas sp. strain PH1 was isolated from soil contaminated with pharmaceutical and dye industry waste. The isolate PH1 could use m-aminophenol as a sole source of carbon, nitrogen, and energy to support the growth. PH1 could degrade up to 0.32 mM m-aminophenol in 120 h, when provided as nitrogen source at 0.4 mM concentration with citrate (0.5 mM) as a carbon source in the growth medium. The presence of ammonium chloride as an additional nitrogen source repressed the degradation of m-aminophenol by PH1. To identify strain PH1, the 16S rDNA sequence was amplified by PCR using conserved eubacterial primers. The FASTA program was used to analyze the 16S rDNA sequence and the resulting homology patterns suggested that PH1 is a Pseudomonas.Key words: m-aminophenol, resorcinol, DNA sequencing.

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