Isolation and characterization of aPseudomonassp. strain PH1 utilizing meta-aminophenol

2000 ◽  
Vol 46 (3) ◽  
pp. 211-217 ◽  
Author(s):  
Razia Kutty ◽  
Hemant J Purohit ◽  
Purushottam Khanna
Keyword(s):  
Nitrogen Source ◽  
16S Rdna ◽  
Sole Source ◽  
Rdna Sequence ◽  
Pseudomonas Sp ◽  
16S Rdna Sequence ◽  
Industry Waste ◽  
Isolation And Characterization ◽  
Dye Industry

Pseudomonas sp. strain PH1 was isolated from soil contaminated with pharmaceutical and dye industry waste. The isolate PH1 could use m-aminophenol as a sole source of carbon, nitrogen, and energy to support the growth. PH1 could degrade up to 0.32 mM m-aminophenol in 120 h, when provided as nitrogen source at 0.4 mM concentration with citrate (0.5 mM) as a carbon source in the growth medium. The presence of ammonium chloride as an additional nitrogen source repressed the degradation of m-aminophenol by PH1. To identify strain PH1, the 16S rDNA sequence was amplified by PCR using conserved eubacterial primers. The FASTA program was used to analyze the 16S rDNA sequence and the resulting homology patterns suggested that PH1 is a Pseudomonas.Key words: m-aminophenol, resorcinol, DNA sequencing.

scholarly journals Erratum to: Deodorization of pig slurry and characterization of bacterial diversity using 16S rDNA sequence analysis

2014 ◽  
Vol 52 (12) ◽  
pp. 1056-1056
Author(s):  
Ok-Hwa Hwang ◽  
Sebastian Raveendar ◽  
Young-Ju Kim ◽  
Ji-Hun Kim ◽  
Tae-Hun Kim ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Bacterial Diversity ◽  
16S Rdna ◽  
Rdna Sequence ◽  
Pig Slurry ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis

16S rDNA Sequence Based Characterization of Bacteria in Stored Jaggery in Indian Jaggery Manufacturing Units

Sugar Tech ◽  
2012 ◽  
Vol 14 (4) ◽  
pp. 422-427 ◽  
Author(s):  
A. K. Verma ◽  
Shubhra Singh ◽  
Shalini Singh ◽  
Ashutosh Dubey
Keyword(s):  
16S Rdna ◽  
Rdna Sequence ◽  
16S Rdna Sequence

Deodorization of pig slurry and characterization of bacterial diversity using 16S rDNA sequence analysis

2014 ◽  
Vol 52 (11) ◽  
pp. 918-929 ◽  
Author(s):  
Ok-Hwa Hwang ◽  
Sebastian Raveendar ◽  
Young-Ju Kim ◽  
Ji-Hun Kim ◽  
Tae-Hun Kim ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Bacterial Diversity ◽  
16S Rdna ◽  
Rdna Sequence ◽  
Pig Slurry ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis

scholarly journals Biochemical characterization of a sphingomonad isolate from the ascocarp of white truffle (Tuber magnatum Pico)

2011 ◽  
Vol 63 (3) ◽  
pp. 697-704 ◽  
Author(s):  
A. Pavic ◽  
S. Stankovic ◽  
Zaklina Marjanovic
Keyword(s):  
Life Cycle ◽  
16S Rdna ◽  
Biochemical Characterization ◽  
Rdna Sequence ◽  
16S Rdna Sequence ◽  
White Truffle ◽  
Tuber Magnatum ◽  
Available Information

Available information on bacteria that influence the economically important white truffle (Tuber magnatum Pico) life cycle is scarce. From the ascocarp of white truffle we isolated a strain TMG 022C, capable for growth in nitrogendepleted conditions and assimilation of mannitol and trehalose. According to 16S rDNA sequence phylogeny, the strain was closely related to Sphingobium amiense. The strain had the ability to perform ammonification, reduce nitrate and solubilize Ca3(PO4)2, produce chitinase, lipase, phospholipase and ?-glucanase, but not cellulase, pectinase, protease and siderophores. The results suggest that Sphingobium sp. TMG 022C could have an influence on the Tuber magnatum life cycle through improved mycelium nutrition and ascocarp decomposition.

scholarly journals Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India)

2019 ◽  
Vol 84 (2) ◽  
pp. 225-236
Author(s):  
Sancho Rajan ◽  
Anwesha Pattanaik ◽  
Venkatesh Kumaresan ◽  
Prasanth Bhatt ◽  
Senthilarasu Gunasekaran ◽  
...  
Keyword(s):  
16S Rdna ◽  
Bay Of Bengal ◽  
Morphological Characteristics ◽  
Sole Source ◽  
Pseudomonas Sp ◽  
16S Rdna Sequencing ◽  
Naphthalene Degradation ◽  
Rdna Sequencing ◽  
Riverine Sediment

Microorganisms capable of using naphthalene as the sole carbon source were isolated from the contaminated sediment of Cooum River. Twenty one isolates were recovered and nine were selected for enrichment due to differences in their morphological characteristics. Out of nine isolates, only four (NS3-SRMND14B, NS14-SRMND14A, NS15-SRMND14D and NS19- -SRMND14E) were capable of completely utilizing naphthalene as the sole source of carbon in carbon free minimal medium (CFMM) supplemented with naphthalene. 16S rDNA sequencing showed that all the four isolates were distantly related to each other and belongs to Bacillus sp. (NS3-SRMND14B), Pseudomonas sp. (NS14-SRMND14A), Cellulosimicrobium sp. (NS15-SRMND14D) and Sphingobacterium sp. (NS19-SRMND14E), respectively. Based on the phylogenetic analysis of 16S rDNA sequencing, the isolate Sphingobacterium sp. (NS19-SRMND14E) has been identified as novel strain. Polymerase chain reaction (PCR) technique showed the presence of naphthalene dioxygenase (ndo) gene responsible for naphthalene degradation only in the Pseudomonas sp. (NS14-SRMND14A). We observed that the ndo gene is not the only gene responsible for naphthalene degradation. Based on our study, the indigenous microorganisms isolated from Cooum Riverine sediment can be used for bioremediation of the polluted sediment along the Bay of Bengal.

16S rDNA Sequence based Phylogenetic Analysis of Some Bacterial Phytoplasma

2012 ◽  
Vol 3 (3) ◽  
pp. 302-304
Author(s):  
G. D.Sharma G. D.Sharma ◽  
◽  
* Dhritiman Chanda ◽  
D.K. Jha D.K. Jha
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rdna ◽  
Rdna Sequence ◽  
16S Rdna Sequence
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