scholarly journals Molecular characterization of COI gene of Ixodes ricinus (Linnaeus, 1758) from Serbia

2014 ◽  
Vol 66 (3) ◽  
pp. 1243-1251
Author(s):  
Sanja Cakic ◽  
Miljana Mojsilovic ◽  
Darko Mihaljica ◽  
Marija Milutinovic ◽  
Andjeljko Petrovic ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Ixodes Ricinus ◽  
Intraspecific Variability ◽  
Balkan Peninsula ◽  
Coi Gene ◽  
Gene Sequences ◽  
Ricinus Tick ◽  
Pathogenic Agents ◽  
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The Ixodes ricinus tick is common in the central part of the Balkan Peninsula. It is a vector of pathogenic agents causing diseases in humans and animals. Little is known about the genetic structure of I. ricinus in this region. We have investigated intraspecific variability of the COI gene among I. ricinus ticks collected from different regions of Serbia, and the correlation between the various types of habitat and genetic variability of ticks. The obtained COI gene sequences are the first barcoding sequences of I. ricinus ticks collected at localities in Serbia. Intraspecific variability of these COI gene sequences was very low, and there was no correlation between the various types of habitat and genetic variability of ticks. Samples from isolated localities (canyon/gorge) showed no genetic differentiations from the majority of samples from open areas. [Projekat Ministarstva nauke Republike Srbije, br. ON 173006] <br><br><font color="red"><b> This article has been retracted. Link to the retraction <u><a href='http://dx.doi.org/10.2298/ABS1404689U'>10.2298/ABS1404689U</a><u></b></font>

scholarly journals Molecular characterization of COI gene of Ixodes ricinus (Linnaeus, 1758) from Serbia

2014 ◽  
Vol 66 (2) ◽  
pp. 683-690 ◽  
Author(s):  
Sanja Cakic ◽  
Miljana Mojsilovic ◽  
D. Mihaljica ◽  
Marija Milutinovic ◽  
A. Petrovic ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Genetic Variability ◽  
Ixodes Ricinus ◽  
Intraspecific Variability ◽  
Balkan Peninsula ◽  
Coi Gene ◽  
Gene Sequences ◽  
Ricinus Tick ◽  
Pathogenic Agents

The Ixodes ricinus tick is common in the central part of the Balkan Peninsula. It is a vector of pathogenic agents causing diseases in humans and animals. Little is known about the genetic structure of I. ricinus in this region. We have investigated intraspecific variability of the COI gene among I. ricinus ticks collected from different regions of Serbia, and the correlation between the various types of habitat and genetic variability of ticks. The obtained COI gene sequences are the first barcoding sequences of I. ricinus ticks collected at localities in Serbia. Intraspecific variability of these COI gene sequences was very low, and there was no correlation between the various types of habitat and genetic variability of ticks. Samples from isolated localities (canyon/gorge) showed no genetic differentiations from the majority of samples from open areas.

scholarly journals Pratylenchus penetrans Parasitizing Potato Crops: Morphometric and Genetic Variability of Portuguese Isolates

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 603
Author(s):  
Diogo Gil ◽  
Joana M.S. Cardoso ◽  
Isabel Abrantes ◽  
Ivânia Esteves
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Phylogenetic Analyses ◽  
Intraspecific Variability ◽  
Its Region ◽  
Genomic Region ◽  
Internal Transcribed Spacers ◽  
Coi Gene ◽  
Pratylenchus Penetrans ◽  
Wide Range

The root lesion Pratylenchus penetrans is an economically important pest affecting a wide range of plants. The morphometry of five P. penetrans isolates, parasitizing potato roots in Portugal, was compared and variability within and between isolates was observed. Of the 15 characters assessed, vulva position (V%) in females and the stylet length in both females/males showed the lowest coefficient of intra and inter-isolate variability. Moreover, DNA sequencing of the internal transcribed spacers (ITS) genomic region and cytochrome c oxidase subunit 1 (COI) gene was performed, in order to evaluate the intraspecific genetic variability of this species. ITS revealed higher isolate genetic diversity than the COI gene, with 15 and 7 different haplotypes from the 15 ITS and 14 COI sequences, respectively. Intra- and inter-isolate genetic diversity was found considering both genomic regions. The differentiation of these isolates was not related with their geographical origin. In spite of the high intraspecific variability, phylogenetic analyses revealed that both ITS region and COI gene separate P. penetrans from other related species. Our findings contribute to increasing the understanding of P. penetrans variability.

scholarly journals Integrative Taxonomy and Molecular Phylogeny of the Plant-Parasitic Nematode Genus Paratylenchus (Nematoda: Paratylenchinae): Linking Species with Molecular Barcodes

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 408
Author(s):  
Phougeishangbam Rolish Singh ◽  
Gerrit Karssen ◽  
Marjolein Couvreur ◽  
Sergei A. Subbotin ◽  
Wim Bert
Keyword(s):  
18S Rrna ◽  
Parasitic Nematode ◽  
Integrative Taxonomy ◽  
Coi Gene ◽  
Integrated Analysis ◽  
Gene Sequences ◽  
Unknown Species ◽  
New Gene ◽  
First Time ◽  
Plant Parasitic

Pin nematodes of the genus Paratylenchus are obligate ectoparasites of a wide variety of plants that are distributed worldwide. In this study, individual morphologically vouchered nematode specimens of fourteen Paratylenchus species, including P. aculentus, P. elachistus, P. goodeyi, P. holdemani, P. idalimus, P. microdorus, P. nanus, P. neoamblycephalus, P. straeleni and P. veruculatus, are unequivocally linked to the D2-D3 of 28S, ITS, 18S rRNA and COI gene sequences. Combined with scanning electron microscopy and a molecular analysis of an additional nine known and thirteen unknown species originating from diverse geographic regions, a total of 92 D2-D3 of 28S, 41 ITS, 57 18S rRNA and 111 COI new gene sequences are presented. Paratylenchus elachistus, P. holdemani and P. neoamblycephalus are recorded for the first time in Belgium and P. idalimus for the first time in Europe. Paratylenchus is an excellent example of an incredibly diverse yet morphologically minimalistic plant-parasitic genus, and this study provides an integrated analysis of all available data, including coalescence-based molecular species delimitation, resulting in an updated Paratylenchus phylogeny and the corrective reassignment of 18 D2-D3 of 28S, 3 ITS, 3 18S rRNA and 25 COI gene sequences that were previously unidentified or incorrectly classified.

BIOLOGICAL IDENTIFICATION OF EUDISTOMA SPECIES (POLYCITORIDAE: ASCIDIACEA) FROM GULF OF MANNAR, INDIA THROUGH COI GENE SEQUENCES

Gene Reports ◽  
2021 ◽  
pp. 101222
Author(s):  
H. Abdul Jaffar Ali ◽  
M.L. Mohammed Kaleem Arshan ◽  
L.K. Praba ◽  
B. Kaleemullah Khan
Keyword(s):  
Coi Gene ◽  
Gulf Of Mannar ◽  
Gene Sequences ◽  
Biological Identification

scholarly journals Prevalence and genetic variability of tick-borne encephalitis virus in host-seeking Ixodes ricinus in northern Italy

2009 ◽  
Vol 90 (12) ◽  
pp. 2877-2883 ◽  
Author(s):  
Giovanna Carpi ◽  
Luigi Bertolotti ◽  
Sergio Rosati ◽  
Annapaola Rizzoli
Keyword(s):  
Genetic Variability ◽  
Ixodes Ricinus ◽  
Evolutionary Dynamics ◽  
Phylogenetic Analyses ◽  
Northern Italy ◽  
Nucleotide Sequence Analysis ◽  
Envelope Gene ◽  
North East ◽  
Tick Borne Encephalitis ◽  
Pcr Targeting

Tick-borne encephalitis (TBE) is a severe disease that has been endemic in north-east Italy since 1992. Over the past two decades, there has been an increase in the number of human cases reported in many European countries, including Italy. To assess the current TBE infection risk, questing ticks were collected from known TBE foci, as well as from a site in northern Italy where no human infections have been reported previously. A total of 1739 Ixodes ricinus (1485 nymphs and 254 adults) was collected and analysed for TBEV prevalence by a real-time RT-PCR targeting the 3′ untranslated region. Phylogenetic analyses of the partial envelope gene were conducted on two newly sequenced TBE virus (TBEV) strains and 28 previously published sequences to investigate the genealogical relationships of the circulating TBEV strains. These phylogenetic analyses confirmed a previous report that the European TBEV subtype is the only subtype circulating within the TBE foci in north-east Italy. Interestingly, nucleotide sequence analysis revealed a high degree of divergence (mean 2.54 %) between the TBEV strains recovered in the Italian province of Trento, despite the circulation of a single TBEV subtype. This elevated genetic variability within a single TBE focus may reflect local differences in the long-standing evolutionary dynamics of TBEV at this site relative to previously characterized sites, or more recent and continuous reintroduction of various TBEV strains.

scholarly journals Diversity of viruses in Ixodes ricinus, and characterization of a neurotropic strain of Eyach virus

2016 ◽  
Vol 11 ◽  
pp. 71-81 ◽  
Author(s):  
S. Moutailler ◽  
I. Popovici ◽  
E. Devillers ◽  
M. Vayssier-Taussat ◽  
M. Eloit
Keyword(s):  
Ixodes Ricinus
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