scholarly journals In vitro plant regeneration of two cucumber (Cucumis sativum L.) genotypes: Effects of explant types and culture medium

Genetika ◽  
2014 ◽  
Vol 46 (2) ◽  
pp. 485-493 ◽  
Author(s):  
Stanislava Grozeva ◽  
Nikolay Velkov
Keyword(s):  
Plant Regeneration ◽  
Culture Medium ◽  
Explant Type ◽  
In Vitro Plant Regeneration ◽  
Morphogenesis In Vitro ◽  
In Vitro Plant

The effect of different phytohormone concentrations on callusogenesis and organogenesis in two cucumber genotypes were studied. It was established that the rate of plant regeneration depends on genotype, explant type and culture medium. Hypocotyls were found to be more responsive than cotyledons in morphogenesis. In vitro planlet-regenerants have been obtained in hypocotyls explants on culture medium with 1.0 and 2.0 mgL-1 BA for cultivar Gergana and in 1.0 and 3.0 mgL-1K-line 15B. Induction of regeneration in cotyledons were established only in cultivar Gergana on culture medium supplemented with 3.0 mgL-1 BA and in combination of 0.5 mgL-1IAA.

scholarly journals In vitro plant regeneration of Passiflora setacea D.C. (Passifloraceae): the influence of explant type, growth regulators, and incubation conditions

2014 ◽  
Vol 50 (6) ◽  
pp. 738-745 ◽  
Author(s):  
Lorena Melo Vieira ◽  
Diego Ismael Rocha ◽  
Mariana Futia Taquetti ◽  
Luzimar Campos da Silva ◽  
José Marcello Salabert de Campos ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Growth Regulators ◽  
Explant Type ◽  
In Vitro Plant Regeneration ◽  
In Vitro Plant

scholarly journals In vitro organogenesis from internodal segments of adult sweet orange plants

2011 ◽  
Vol 46 (6) ◽  
pp. 672-674 ◽  
Author(s):  
Meire Menezes Bassan ◽  
Francisco de Assis Alves Mourão Filho ◽  
Luzia Yuriko Miyata ◽  
Beatriz Madalena Januzzi Mendes
Keyword(s):  
Plant Regeneration ◽  
In Vitro Culture ◽  
Culture Medium ◽  
Sweet Orange ◽  
Naphthaleneacetic Acid ◽  
In Vitro Plant Regeneration ◽  
Apparent Effect ◽  
In Vitro Organogenesis ◽  
In Vitro Plant

The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and 'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27ºC in the absence of light for 50 days, followed by a 16-hour photoperiod for 20 days. Regeneration was assessed 50 and 70 days after in vitro culture. Organogenesis in cultivars Hamlin and Valência was promoted by EME supplemented with BAP, while NAA showed no apparent effect.

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