Investigating class II HLA antigens' association with insulin-dependent diabetes mellitus in population of Vojvodina

2003 ◽  
Vol 56 (1-2) ◽  
pp. 26-31
Author(s):  
Svetlana Vojvodic
Keyword(s):  
Diabetes Mellitus ◽  
Relative Risk ◽  
Hla Antigens ◽  
Insulin Dependent Diabetes Mellitus ◽  
Class Ii ◽  
Insulin Dependent Diabetes ◽  
Dependent Diabetes Mellitus ◽  
Control Group ◽  
Significant Difference ◽  
Insulin Dependent

Introduction Class II HLA antigens were investigated in a group of 28 patients with insulin-dependent diabetes mellitus (IDDM) and 218 healthy unrelated persons (control group) from Vojvodina. Material and methods We used a modified two-colour immunofluorescence method (serologic technique) to determine the phenotype of DR and DQ locus HLA antigens. Phenotype frequencies of class II HLA antigens were determined in both investigated groups and were used for calculating relative risk (RR). If RR was higher than 1, we calculated the population attributable risk (EF), and if RR was lower than 1, we calculated the preventive fraction (PF). Investigation of statistically significant differences in frequencies of class II HLA antigens in patients and control group was performed by using ?2 test. Results Results of investigation showed that values of RR were higher than 1 for HLA DR4 (2,808), DR10 (1,116) and DQ3 (1,386), while we noticed a statistically significant difference in frequencies of HLA DR4 (?2 test: 4,805) in patients regarding control group. HLA DQ1 antigen has a preventive role in development of IDDM due to highest value of PF (0,314). Conclusion Results of our investigation confirm that there is an association of HLA DR4 with IDDM in population of Vojvodina. High values of relative risk of IDDM, noticed in persons with HLA DR4 antigen, point to the degree of risk of IDDM, which is a disease with great socioeconomic importance in Vojvodina.

HLA Antigens and Immunoresponsiveness to Insulin in Insulin-Dependent Diabetes Mellitus

2008 ◽  
Vol 8 (1) ◽  
pp. 13-19 ◽  
Author(s):  
J. Bertrams ◽  
F.K. Jansen ◽  
D. Gruneklee ◽  
H.E. Reis ◽  
H. Drost ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Hla Antigens ◽  
Insulin Dependent Diabetes Mellitus ◽  
Insulin Dependent Diabetes ◽  
Dependent Diabetes Mellitus ◽  
Insulin Dependent

Distinct Associations of HbA1c and the Urinary Excretion of Pentosidine, an Advanced Glycosylation End-product, with Markers of Endothelial Function in Insulin-dependent Diabetes mellitus

1998 ◽  
Vol 80 (07) ◽  
pp. 52-57 ◽  
Author(s):  
Ronald Smulders ◽  
Casper Schalkwijk ◽  
Ab Donker ◽  
Victor van Hinsbergh ◽  
Johan TeKoppele ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Endothelial Function ◽  
Urinary Excretion ◽  
Insulin Dependent Diabetes Mellitus ◽  
Insulin Dependent Diabetes ◽  
Dependent Diabetes Mellitus ◽  
Control Group ◽  
Advanced Glycosylation End Products ◽  
Insulin Dependent ◽  
Advanced Glycosylation

SummaryDysfunction of the vascular endothelium is considered an early step in the development of diabetic angiopathy. Hyperglycaemia results in endothelial dysfunction, both through direct effects of glucose and through formation of advanced glycosylation end-products (AGEs). We hypothesized that the effects of glucose and AGEs on endothelial function in insulin-dependent diabetes mellitus (IDDM) are distinct and are reflected by distinct plasma markers of endothelial function. We therefore measured plasma levels of von Willebrand factor (vWF), soluble (s) E-selectin and vascular cell adhesion molecule-1 (sVCAM-1), and evaluated the relationship with HbA1c and urinary excretion of pentosidine, an AGE product, in 56 patients with IDDM. Urinary pentosidine excretion was higher in the diabetic than in a control group (n = 60) of similar age (P <0.0001) and showed a steeper increase with age (P <0.02 vs controls). In the diabetic group, sE-selectin was correlated to HbA1c (r = 0.52, P <0.0001), whereas sVCAM-1 was not (r = 0.11, P = 0.47). In contrast, sVCAM-1 showed a trend towards a correlation with log (pentosidine excretion) (r = 0.27, P = 0.06), whereas sE-selectin did not (r = –0.16, P = 0.27). Log(vWF) was correlated to HbA1c (r = 0.50, P <0.0001) and tended to correlate with log (pentosidine excretion) (r = 0.25, P = 0.07). Multivariate analyses with both pentosidine and HbA1c as independent variables showed significant associations of sE-selectin with HbA1c, of sVCAM-1 with pentosidine, and of log(vWF) with both HbA1c and pentosidine (all P-values <0.02). Our results imply that the effects of glucose and AGEs on the endothelium can be reflected by distinct endothelial markers. Plasma sE-selectin may reflect short-term effects of glucose on the endothelium, sVCAM-1 the effects of AGEs, and vWF the combined effect of glucose and AGEs.

scholarly journals Penetration of Ciprofloxacin into the Interstitial Space of Inflamed Foot Lesions in Non-Insulin-Dependent Diabetes Mellitus Patients

1999 ◽  
Vol 43 (8) ◽  
pp. 2056-2058 ◽  
Author(s):  
Markus Müller ◽  
Martin Brunner ◽  
Ursula Hollenstein ◽  
Christian Joukhadar ◽  
Rainer Schmid ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Insulin Dependent Diabetes Mellitus ◽  
Insulin Dependent Diabetes ◽  
Dependent Diabetes Mellitus ◽  
Time Curve ◽  
Concentration Time ◽  
Significant Difference ◽  
Tissue Area ◽  
Insulin Dependent ◽  
Foot Lesions

ABSTRACT Interstitial ciprofloxacin concentrations were measured by microdialysis in inflamed foot lesions of non-insulin-dependent diabetes mellitus patients following intravenous administration of 0.2 g of ciprofloxacin. Interstitial ciprofloxacin concentrations were significantly lower than corresponding serum concentrations. There was no significant difference in the penetration of ciprofloxacin into inflamed and unaffected tissue (area under the concentration-time curveinfection/area under the concentration-time curveunaffected tissue = 0.99 ± 0.15 [mean ± standard error], n = 6). Thus, inflammation appears to have little or no effect on the penetration of ciprofloxacin into tissue.

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