MECHANISM OF MOVEMENT OF EPIDERMIS, ESPECIALLY ITS MELANOPHORES, IN WOUND HEALING, AND BEHAVIOR OF SKIN GRAFTS IN FROG TADPOLES

Abstract Background Wound repair is one of the most complex biological processes of human life. Allogeneic cell-based engineered skin substitutes provide off-the-shelf temporary wound coverage and act as biologically active dressings, releasing growth factors, cytokines and extracellular matrix components essential for proper wound healing. However, they are susceptible to immune rejection and this is their major weakness. Thanks to their low immunogenicity and high effectiveness in regeneration, fetal skin cells represent an attractive alternative to the commonly used autologous and allogeneic skin grafts. Methods/design We developed a new dressing comprising a collagen matrix seeded with a specific ratio of active fetal fibroblasts and keratinocytes. These produce a variety of healing growth factors and cytokines which will increase the speed of wound healing and induce an immunotolerant state, with a slight inflammatory reaction and a reduction in pain. The objective of this study is to demonstrate that the use of this biological dressing for wound healing at the split-thickness skin graft (STSG) donor site, reduces the time to healing, decreases other co-morbidities, such as pain, and improves the appearance of the scar. This investigation will be conducted as part of a randomized study comparing our new biological dressing with a conventional treatment in a single patient, thus avoiding the factors that may influence the healing of a graft donor site. Discussion This clinical trial should enable the development of a new strategy for STSG donor-wound healing based on a regenerative dressing. The pain experienced in the first few days of STSG healing is well known due to the exposure of sensory nerve endings. Reducing this pain will also reduce analgesic drug intake and the duration of sick leave. Our biological dressing will meet the essential need of surgeons to “re-crop” from existing donor sites, e.g., for thermal-burn patients. By accelerating healing, improving the appearance of the scar and reducing pain, we hope to improve the conditions of treatment for skin grafts. Trial registration ClinicalTrials.gov, ID: NCT03334656. Registered on 7 November 2017.

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Phlegmon of the Foot As A Camel Bite Complication

Polish Journal of Surgery ◽

10.2478/pjs-2014-0086 ◽

2015 ◽

Vol 86 (10) ◽

Author(s):

Bartosz Skonieczny ◽

Wojciech Skonieczny

Keyword(s):

Wound Healing ◽

Conservative Treatment ◽

Motor Function ◽

Rare Case ◽

Daily Practice ◽

Skin Grafts ◽

Exotic Animals ◽

Complete Wound Healing ◽

At Home

AbstractThe great popularity of foreign excursions and travel in exotic directions, as well as increasing popularity of breeding exotic animals at home, is evidence that in daily practice one may observe injuries inflicted by animals atypically occurring in Poland.The study presented and described a rare case of a patient attacked by a camel living in an agro-tourism farm in our country. Thanks to the combination of surgical and conservative treatment complete wound healing was observed, including the skin grafts, with preserved motor function of the foot.

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A Retrospective Study on the Use of Dermis Micrografts in Platelet-Rich Fibrin for the Resurfacing of Massive and Chronic Full-Thickness Burns

Stem Cells International ◽

10.1155/2019/8636079 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Alessandro Andreone ◽

Daan den Hollander

Keyword(s):

Wound Healing ◽

Retrospective Study ◽

Medical Device ◽

Skin Graft ◽

Skin Grafts ◽

Full Thickness ◽

Platelet Rich Fibrin ◽

Burn Wounds ◽

Complete Wound Healing ◽

Dermal Template

The coverage of massive burns still represents a big challenge, even if several strategies are to date available to deal with this situation. In this study, we describe the use of a combination of platelet-rich fibrin and micrograft spray-on skin in order to increase the yield of grafted cells in patients. We treated a total of five patients, of which two were affected by massive burns and three with chronic burn wounds. Briefly, autologous micrografts were obtained by Rigenera technology using a class I medical device called Rigeneracons. The micrografts were then combined with PRF and sprayed on the wound bed by a Spraypen. Before applying PRF/micrograft spray-on skin, the wound bed was covered with an Integra® dermal template, and the wounds were dressed with a layer of antimicrobial dressing applied directly over the silicone layer. When the silicone layer of the dermal template started showing signs of separation, the wound was considered ready for grafting. In all cases, we observed a fast and complete skin graft on average after 7-10 days by PRF/micrograft spray-on skin treatment. In particular, two patients with massive burns reported rapid reepithelialization, and three patients with chronic burn wounds, two of whom had failed skin grafts before the procedure, had complete wound healing within a week. In conclusion, the results showed in this study suggest that the use of PRF/micrograft spray-on skin represents a promising approach in the management of burns or chronic burn wounds.

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Role of serum amyloid P in skin graft survival and wound healing in burned patients receiving skin grafts

Clinica Chimica Acta ◽

10.1016/j.cca.2010.09.036 ◽

2011 ◽

Vol 412 (3-4) ◽

pp. 227-229 ◽

Cited By ~ 4

Author(s):

Yi-ming Zhang ◽

Ya-dong Fang ◽

Yi-cheng Wang ◽

Shao-liang Wang ◽

Ze-yuan Lei ◽

...

Keyword(s):

Wound Healing ◽

Graft Survival ◽

Skin Graft ◽

Serum Amyloid ◽

Skin Grafts ◽

Serum Amyloid P ◽

Amyloid P

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Fish skin grafts compared to human amnion/chorion membrane allografts: A double‐blind, prospective, randomized clinical trial of acute wound healing

Wound Repair and Regeneration ◽

10.1111/wrr.12761 ◽

2019 ◽

Vol 28 (1) ◽

pp. 75-80 ◽

Cited By ~ 1

Author(s):

Robert S. Kirsner ◽

David J. Margolis ◽

Baldur T. Baldursson ◽

Kristin Petursdottir ◽

Olafur B. Davidsson ◽

...

Keyword(s):

Clinical Trial ◽

Wound Healing ◽

Randomized Clinical Trial ◽

Skin Grafts ◽

Fish Skin ◽

Double Blind ◽

Human Amnion ◽

Prospective Randomized Clinical Trial ◽

Acute Wound

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Skin Epidermis and Adnexa Regrowth Induced by Treatment With Artificial Dermal Template After Full-Thickness Skin Wound

The International Journal of Lower Extremity Wounds ◽

10.1177/1534734618818909 ◽

2019 ◽

Vol 18 (1) ◽

pp. 42-55

Author(s):

Binghui Li ◽

Hang Xue ◽

Xiaobo Zhao ◽

Yuxiong Weng ◽

Gongchi Li ◽

...

Keyword(s):

Wound Healing ◽

Wnt Signaling ◽

Skin Wound ◽

Hair Follicles ◽

Skin Grafts ◽

Full Thickness ◽

Thickness Skin ◽

Distal Edge ◽

Dermal Template ◽

Skin Epidermis

Full-thickness skin wounds are common accidents. Although healing can be achieved by treatments like autologous skin grafts, donor site morbidity is hardly evitable. In this article, we provide compelling evidence demonstrating that artificial dermal template (ADT)-treated wound healing is achieved by regrowth of skin epidermis as well as adnexa without skin grafts by use of rodent models. First, by fixating a chamber to the wound edge, we confirmed that wound healing was achieved by regeneration instead of contracture. We found highly proliferative cells in adnexa in the newly formed skin. In the distal edge of newly formed skin, we identified immature hair follicles at early developing stages, suggesting they were newly regenerated. Second, we observed that the Lgr5-positive hair follicle stem cells contributed to formation of new hair follicles through a lineage tracing model. Also, Lgr6-positive cells were enriched in distal edge of newly developed skin. Finally, WNT signaling pathway mediators were highly expressed in the new skin epidermis and adnexa, implying a potential role of WNT signaling during ADT treatment-stimulated skin regrowth. Taken together, our findings demonstrated that full skin regrowth can be induced by ADT treatment alone, thus arguing for its wide clinical application in skin wound treatment.

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