Application of the Agar Gel Precipitation Test to Serological Studies of Chickens Inoculated with Haemophilus gallinarum

1965 ◽  
Vol 9 (4) ◽  
pp. 591 ◽  
Author(s):  
S. Sato ◽  
M. Shifrine
Keyword(s):  
Agar Gel ◽  
Serological Studies ◽  
Precipitation Test

scholarly journals Serological Studies of Bovine Ureaplasmas by Agar-Gel Precipitation Test

1980 ◽  
Vol 42 (1) ◽  
pp. 9-15,17
Author(s):  
Hiroshi NAGATOMO ◽  
Hitoshi KOTANI ◽  
Manabu OGATA ◽  
Takamasa SHIMIZU
Keyword(s):  
Agar Gel ◽  
Serological Studies ◽  
Precipitation Test

AN AGAR-GEL PRECIPITATION TEST FOR THE LABORATORY DIAGNOSIS OF SMALLPOX

The Lancet ◽  
1959 ◽  
Vol 273 (7079) ◽  
pp. 916-917 ◽  
Author(s):  
K.R. Dumbell ◽  
MD. Nizamuddin
Keyword(s):  
Laboratory Diagnosis ◽  
Agar Gel ◽  
Precipitation Test

scholarly journals A preliminary survey of the epidemiology of bluetongue in Costa Rica and Northern Colombia

1985 ◽  
Vol 94 (3) ◽  
pp. 357-363 ◽  
Author(s):  
E. J. Homan ◽  
H. Lorbacher de Ruiz ◽  
A. P. Donato ◽  
W. P. Taylor ◽  
T. M. Yuill
Keyword(s):  
Costa Rica ◽  
Virus Infection ◽  
Costa Rican ◽  
Preliminary Survey ◽  
Virus Antibody ◽  
Antibody Prevalence ◽  
Agar Gel ◽  
Serologic Survey ◽  
Precipitation Test

SUMMARYRecent evidence of bluetongue (BT) virus infection of livestock in scattered localities in the neotropics prompted a serologic survey of cattle in Colombia and Costa Rica. In Costa Rica 48·1% of 1435 bovine animals had BT virus antibody in the agar gel precipitation test (AGPT). In Colombia 51·8% of 635 cattle were AGPT-positive for BT virus. Antibody prevalence ranged from over 50% in the lowlands to 0% in Costa Rica and 19% in Colombian cattle above 2000 m altitude. Neutralization tests indicated that Costa Rican cattle had been exposed to BT virus types 6, 12, 14 and 17.

scholarly journals Purifikasi Imunoglobulin Yolk Pada Ayam yang Divaksin terhadap Ekskretori/Sekretori Stadium L3 Ascaridia galli

2010 ◽  
Vol 10 (2) ◽  
pp. 9-15
Author(s):  
Darmawi Darmawi ◽  
Ummu Balqis ◽  
Risa Tiuria ◽  
Muhammad Hambal ◽  
Samadi Samadi
Keyword(s):  
Good Choice ◽  
Ascaridia Galli ◽  
Agar Gel ◽  
Secretory Antigen ◽  
Egg Yolks ◽  
Fast Performance Liquid Chromatography ◽  
Excretory Secretory Antigen ◽  
Incomplete Antibody ◽  
Precipitation Test

Purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli L3 larvae stageABSTRACT. The main immunoglobulin fraction of poultry is called IgY, in order to distinguish it from the mammalian IgG. This article focus on purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli larvae to obtained purity IgY. Active vaccinations with excretory/secretory antigen were applied intra muscularly of chickens with an initial dose of 80 μg. The vaccinations were repeated three times with dose of each 60 μg with an interval of one week. The first vaccinations were excretory/secretory antigen mixed with Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete. Antibody response in yolk was detected at weekly intervals by agar gel precipitation test (AGPT). The chicken’s eggs were collected from 49 day after vaccinations. IgY was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ekscretory/secretory IgY protein was determined by Bradford method (λ = 280 nm). The result showed that antibody in yolk was begun detect with AGPT at four weeks after vaccination. IgY concentration after purification was 0,875 ± 0.251 mg/ml. This study has shown that the product released in vitro by L3 stage A. galli is capable of stimulating humoral immunity by mean of producing antibody through yolk as biologic manufacturer could be a good choice.

Conventional and Molecular Detection of Avipoxviruses from Chickens, Pigeons and Turkeys

2019 ◽  
Vol 20 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Samah Mosad
Keyword(s):  
Molecular Detection ◽  
Chain Reaction ◽  
Cutaneous Lesions ◽  
Agar Gel ◽  
Hyperimmune Serum ◽  
Polymerase Chain ◽  
Indirect Immunoperoxidase ◽  
Pooled Samples ◽  
Positive Results ◽  
Precipitation Test

In the present study, a total of 90 cutaneous lesions samples were collected from chickens, pigeons, and turkeys farms in Dakahlia Governorate, Egypt during summer 2016. These farms suspected to be infected with Avipoxviruses (APVs).Thirty pooled samples were created (10 from chickens, 10 from pigeons and 10 from turkeys). Hyperimmune serum was prepared against standard fowlpox virus in adult white New Zealand rabbits. APV were identified in the collected samples using agar gel precipitation test (AGPT), indirect immunoperoxidase, and polymerase chain reaction (PCR) based on 4b gene of APVs. The results revealed that out of 30 tested samples there were 16 samples (53.3%) tested positive via AGPT including, 6 chicken samples (60%) , 5 pigeon samples (50%) and 5 turkey samples (50%). while using indirect immunoperoxidase, positive results were detected in 23 samples (76.7%) including, 8 chicken samples (80%), 8 pigeon samples (80%) and 7 turkey samples (70%).The 4b gene of APVs was detected using PCR in all tested samples (100%). In conclusion, Indirect immunoperoxidase is superior over AGPT in APVs detection in collected samples from chickens, pigeons and turkeys. PCR could be efficiently used in molecular diagnosis of the virus.

A Micro-Method for the Agar-Gel Precipitation Test for Histoplasmosis

1963 ◽  
Vol 87 (4) ◽  
pp. 592-593 ◽  
Author(s):  
Milton Goldin ◽  
Shirley McMillen
Keyword(s):  
Agar Gel ◽  
Precipitation Test
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