Identifikasi Sifat Kuantitatif dan Sifat Kualitatif pada Sapi Aceh Dalam Rangka Pelestarian Sumber Daya Genetik Ternak Lokal

ABSTRACT. Sapi Aceh merupakan sumber daya genetik ternak lokal dan merupakan rumpun sapi lokal Indonesia yang tersebar di Provinsi Aceh, dan telah ditetapkan berdasarkan Keputusan Menteri Pertanian nomor: 2907/Kpts/OT.140/6/2011 pada 17 Juni 2011. Penelitian ini bertujuan untuk membandingkan keragaman sapi Aceh di Kabupaten Aceh Besar saat ini dengan SNI 7651.3:2013. Penelitian ini dilaksanakan pada bulan Januari – April 2020. Jumlah sampel sapi Aceh jantan berumur 24-36 bulan sebanyak 62 ekor dan 106 ekor sapi Aceh betina berumur 15-18 bulan. Peubah yang diamati (1) tinggi pundak (TP), (2), panjang badan (PB). dan (3) lingkar dada (LD). Penentuan sampel menggunakan metode purposive sampling. Berdasarkan hasil penelitian, nilai sifat kuantitatif sapi Aceh jantan dan betina masing-masing memiliki tinggi pundak (TP) 108,08±3,59 cm dan 89,53±4,26 cm, panjang badan (PB) 110,26±4,92 cm dan 88,77±6,52 cm dan lingkar dada (LD) 141,02±7,34 cm dan 107,22±8,92 cm. Sebanyak 48,39 % sapi Aceh jantan termasuk kategori kelas III dan 30,19 % sapi Aceh betina termasuk kategori kelas II berdasarkan SNI 7651.3:2013. Sifat kualitatif bentuk muka sapi Aceh jantan dan betina secara keseluruhan berbentuk cekung dengan persentase 80,65 % dan 90,57 %. Sementara tanduk pada sapi Aceh jantan berbentuk ke samping melengkung ke atas dengan rataan persentase 51,61 % dan sapi Aceh betina secara umum hanya membentuk lingkaran tanduk pendek dengan rataan persentase sebesar 67,92 %. Bentuk garis punggung sapi Aceh jantan dan betina berbentuk cekung dengan persentase sebesar 72,58 % dan 79,25 %. ((Identification of quantitative traits and qualitative traits in Aceh cattle in the context of preserving animal genetic resources) ABSTRAK. Aceh cattle are a genetic resource for local livestock and are a clump of local Indonesian cattle spread across Aceh Province, and have been determined based on the Decree of the Minister of Agriculture number: 2907 / Kpts / OT.140 / 6/2011 on 17 June 2011. This study aims to compare the diversity of Aceh cattle in Aceh Besar district currently with SNI 7651.3: 2013. This research was conducted in January - April 2020. The total samples of male Aceh cattle aged 24-36 months were 62 cows and 106 female Aceh cattle aged 15-18 months. The variables observed were (1) shoulder height (TP), (2), body length (PB). and (3) chest circumference (LD). Determination of the sample using purposive sampling method. Based on the results of the study, the quantitative traits of male and female Aceh cattle each had shoulder height (TP) 108.08 ± 3.59 cm and 89.53 ± 4.26 cm, body length (PB) 110.26 ± 4, 92 cm and 88.77 ± 6.52 cm and chest circumference (LD) 141.02 ± 7.34 cm and 107.22 ± 8.92 cm. A total of 48.39% of Aceh male cattle are in class III category and 30.19% of female Aceh cattle are categorized as class II based on SNI 7651.3: 2013. The qualitative characteristics of the face shape of male and female Aceh cattle are overall concave with a percentage of 80.65% and 90.57%. While the horns on male Aceh cattle are curved sideways upward with an average percentage of 51.61% and female Aceh cattle in general only form a short horn circle with an average percentage of 67.92%. The form of the back line of male and female Aceh cattle is concave with a percentage of 72.58% and 79.25%.

Histologi, Histomorfometri, dan Histokimia Usus Ayam Buras (Gallus gallus domesticus) Selama Periode Sebelum dan Setelah Menetas

ABSTRACT. Usus adalah salah satu organ pencernaan yang berperan penting pada proses absorpsi nutrisi sebagai sumber energi. Penelitian ini bertujuan mengetahui histologi, histomorfometri dan histokimia sebaran glikogen pada usus ayam buras (Gallus gallus domesticus) sebelum dan setelah menetas menggunakan pewarnaan Haematoksilin-Eosin (HE) dan pewarnaan Periodic Acid Schiff (PAS). Sampel penelitian terdiri atas empat kelompok tingkat umur berbeda, setiap kelompok berjumlah enam usus ayam. Hasil penelitian menunjukkan perbedaan nyata (P˂0,05) morfometri usus ayam buras secara makroskopis. Secara histologis pada setiap perkembangan sudah terlihat lapisan usus yang terdiri atas tunika mukosa, submukosa, muskularis, dan serosa. Pada tunika mukosa, didapati tiga lamina yaitu lamina epitelia, propria, dan muskularis mukosa yang mulai teramati pada masa inkubasi hari ke-14. Lamina epitelia berupa sel epitel silindris selapis dengan mikrovili, lamina propria berupa jaringan ikat, dan lamina muskularis mukosa berupa berkas otot polos. Pada tunika mukosa dijumpai adanya sel Goblet dan kripta Liberkuhn yang teramati pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7. Hasil histomorfometri ketebalan lapisan usus, panjang vili, lebar vili, dan diameter lumen usus ayam buras pada masa inkubasi dan setelah menetas menunjukkan perbedaan nyata (P˂0,05). Temuan reaksi positif kuat terhadap pewarnaan PAS dijumpai pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7 dan positif lemah pada masa inkubasi hari ke-14. Dapat disimpulkan bahwa terdapat korelasi antara perkembangan usus ayam secara makroskopis, histomorfometri ketebalan tunika dan vili, serta sebaran reaksi positif kuat terhadap pewarnaan PAS pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7. (Histological, histomorphometrical, and histochemical of intestine on native chicken (Gallus gallus domesticus) during pre and posthatch period) ABSTRAK. The intestines are one of the digestive organ that play an important role in the nutrient absorption process as an energy source. The aim of this study was to determine the histomorphometric and histochemical of glycogen distribution in the intestines of native chickens pre and post hatching periods using Haematoxylin-Eosin (HE) staining and Periodic Acid Schiff (PAS) staining. The samples of this study was consisted of four different age groups, each group consisting of six chicken intestines. The results showed a significant difference (P˂0.05) on macroscopic morphometry of the native chicken’s intestine. Histologically in each group has been found the intestines consisting of mucosa, submucous, muscularis, and serous tunic. In the mucosal tunic, there were three layers; ephitelial, propria, and muscularial mucous can be observed during the 14th day of incubation period. Ephitelial layer in the form of cylindrical epithelial cells with microvilli, proprial layer in the form of connective tissue, and layer of the muscularis mucosa form smooth muscle thread. In the mucosal tunic, there was Goblet cells and Liberkuhn crypts that can be observed during the 20th day incubation period and 7th day post hatch. Histomorphometry results of intestinal lining thickness, villi length, villi width, and lumen diameter of native chicken intestine during incubation and post hatching showed significant differences (P˂0.05). The findings of a strong positive reaction to the staining of PAS were found on the incubation period of the 20th day and post hatching the 7th day and positively weak on the incubation period of the 14th day. It can be concluded that there is a correlation between macroscopic development of chicken intestine, histomorphometric of tunica and villous thickness, as well as the distribution of strong positive reactions to the staining of PAS during the incubation period of the 20th day and post hatching the 7th day.

Populasi Bakteri Usus Halus dan Performan Ayam Kampung Silangan Kampung-Leghorn Akibat Ditambahkan Ekstrak Umbi Dahlia dalam Ransum

ABSTRACT. Penelitian bertujuan untuk mengkaji perkembangan bakteri usus halus dan performan ayam kampung silangan kampung-leghorn (KL) akibat ditambahkan ekstrak umbi dahlia dalam ransum. Ternak percobaan yang digunakan adalah persilangan ayam kampung silangan KL sebanyak 200 ekor umur 22 hari dengan rata-rata bobot badan 180,46 ± 1,21 g. Bahan perlakuan meliputi umbi dahlia, ethanol 70%, kertas saring halus serta bahan pakan penyusun ransum (jagung giling, bekatul, bungkil kedelai, tepung ikan, CaCO3 dan mineral dan vitamin mix). Rancangan percobaan yang digunakan rancangan acak lengkap dengan 4 perlakuan dan 5 ulangan (masing-masing unit percobaan diisi 10 ekor). Perlakuan yang diterapkan meliputi: P0=Ransum kontrol/RK, P1=RK+0,39% ekstrak umbi dahlia, P2=RK+0,78 ekstrak umbi dahlia, dan P3= RK+1,17 ekstrak umbi dahlia. Parameter yang diukur meliputi populasi bakteri asam laktat, Escherichia coli, pH digesta tiap masing-masing segmen usus halus dan pertambahan bobot badan harian (PBBH). Data dianalisis dengan menggunakan uji sidik ragam dan jika berpengaruh nyata dilakukan uji beda nyata Duncan pada taraf 5%. Hasil penelitian menunjukkan bahwa penambahan ekstrak umbi dahlia berpengaruh nyata terhadap populasi bakteri asam laktat, Escherichia coli, pH digesta tiap masing-masing segmen usus halus dan PBBH. Kesimpulan adalah penambahan ekstrak umbi dahlia sampai taraf 1,17% sebagai sumber inulin di dalam ransum dapat meningkatkan populasi bakteri asam laktat, menurunkan pH dan Escherichia coli pada masing-masing segmen usus halus serta memperlambat laju digesta dan meningkatkan PBBH. (Population of small intestine bacteria and performance of native chicken-leghorn crossbreed duo to feeding of dahlia tuber extract) ABSTRAK. This study aims to examine the development of small intestine bacteria and the performances of native chicken-leghorn crossbreed due to the addition of dahlia tuber extract in the diet. Experimental animals were 200 unsex native chicken-leghorn crossbreed, 22 days old with an average body weight of 180.46 ± 1.21 g. Treatment materials include dahlia tuber, ethanol 70%, fine filter paper and feed stuff (yellow corn, rice bran, soy bean meal, fish meal, CaCO3, and vitamin-mineral mix). The present experiment was assigned in a completely randomized with 4 treatments and 5 replications (10 birds each). The treatments were: P0=Control Diet/CD, P1=CD+0,39% dahlia tuber extract, P2=CD+0,78% dahlia tuber extract and P3=CD+1,17% dahlia tuber extract. Parameters measured were the number of Lactic Acid Bacteria (LAB), Escherichia coli, intestinal potential hydrogen (pH) (duodenal, jejunal and ileal), rate of passage and daily body weight gain (DBWG). Data were subjected to ANOVA and followed by Duncan Multiple Range Test on 5% level. The results indicated that feeding native chicken-leghorn crossbreed with the diet containing dahlia tuber extract (DTE) significantly (P0.05) increased LAB population and DBWG, decreased E. coli population and intestinal pH, and slow the rate of passage down. In conclusion, feeding of dahlia tubers as a source of inulin in the form of dahlia tuber extract to a level of 1.17% increased the population of lactic acid bacteria, reduced pH and Escherichia coli in each segment of the small intestine and retard the rate of digestion and increased body weight gain.

Protein-Energy Synchronization Index of Various Energy Source of Feed Concentrate for Ruminants

ABSTRACT. The study aimed to determine the protein-energy synchronization (PES) index of the various energy source of feed concentrate in vitro, as a database for the preparation of ruminant rations based on the PES index. The research was conducted from May to August 2020. The research was carried out experimentally through three stages: proximate analysis, in vitro digestibility test, and index calculation. The materials used were rumen fluid of three Jawa Randu Goats, taken shortly after being slaughtered at Sokaraja Goat Slaughterhouse, and seven types of energy sources of feed concentrate. Each feedstuff was duplicated for 3 replications, then the digestibility data for organic matter and protein of each feedstuff were collected at the 2nd, 4th, 6th, 8th, 12th, 24th, and 48th hour by in vitro fermentation. The data collection results were then regressed and the results were included in the PES index equation. The variable measured was the PES synchronization index. The results showed that the PES index of rice bran was 0.54, rice polish 0.50, pollard 0.57, corn 0.87, dried cassava 0.94, dried cassava dreg 0.90, and bread flour 0.94. The study concluded that the energy source of feed concentrate are potential as the medium to the high category of PES index values in the preparation of ruminant rations, where corn, dried cassava, dried cassava dreg, and bread flour have a PES index in the high category, while rice bran, rice polish, and pollard in the medium category. (Indeks sinkronisasi protein-energi berbagai bahan pakan konsentrat sumber energi bagi ruminansia) ABSTRAK. Tujuan penelitian ini adalah menginventarisasi indeks sinkronisasi protein-energi (SPE) berbagai bahan pakan konsentrat sumber energy, sebagai basis data penyusunan ransum ruminansia berbasis indeks SPE secara in vitro. Penelitian dilaksanakan mulai bulan Mei hingga Agustus 2020. Penelitian dilaksanakan secara eksperimental melalui tiga tahap yaitu analisis proksimat, kecernaan in vitro, dan perhitungan indeks. Materi yang digunakan adalah cairan rumen 3 Kambing Jawa Randu yang diambil sesaat setelah disembelih di Rumah Potong Hewan Sokaraja, serta 7 jenis bahan pakan konsentrat sumber energi. Masing-masing bahan pakan diduplikasi sebanyak 3 ulangan, kemudian masing-masing diukur kecernaan bahan organik dan protein pada waktu fermentasi in vitro ke 2, 4, 6, 8, 12, 24 dan 48 jam. Data yang dikoleksi selanjutnya di uji regresi dan hasilnya dimasukkan dalam persamaan indeks SPE. Variabel yang diukur adalah indeks SPE. Hasil penelitian menunjukkan bahwa indeks SPE dedak sebesar 0,54, bekatul 0,50, pollard 0,57, jagung 0,87, gaplek 0,94, onggok kering 0,90 dan tepung roti 0,94. Penelitian menyimpulkan bahwa, konsentrat sumber energi memiliki potensi nilai indeks sinkronisasi protein-energi pada kategori sedang hingga tinggi dalam penyusunan ransum ruminansia. Jagung, gaplek, onggok kering dan tepung roti memiliki indeks sinkronisasi protein-energi pada kategori tinggi, sedangkan dedak, bekatul dan pollard pada kategori sedang.

Pengaruh Pemberian Ekstrak Daun Gaharu (Grynops versteegii) terhadap Stres Transportasi pada Broiler Jantan

ABSTRACT. Penelitian bertujuan mengetahui pengaruh penambahan ekstrak daun Gaharu (Grynops verstegii) sebelum transportasi terhadap stres transportasi dan penyusutan bobot badan pada broiler jantan. Materi yang digunakan sebagai sampel yaitu 60 ekor dari 200 ekor broiler jantan yang dipelihara, dan ekstrak daun Gaharu dari hasil destilasi. Penelitian menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL) dengan uji lanjut Duncan’s. Broiler jantan yang digunakan dalam penelitian terbagi menjadi 4 perlakuan level pemberian ekstrak daun Gaharu yang berbeda. Pemberian ekstrak daun Gaharu berdasarkan pada bobot badan (BB) broiler, yaitu P0 (tanpa pemberian ekstrak daun Gaharu sebagai kontrol ), P1 (250 mg/kg BB), P2 (300 mg/kg BB), P3 (350 mg/kg BB), setiap perlakuan memiliki lima ulangan dan setiap ulangan menggunakan 3 ekor. Parameter yang diamati adalah penyusutan bobot badan, frekuensi denyut jantung, suhu tubuh, dan frekuensi Pernafasan. Hasil penelitian menunjukkan bahwa penambahan ekstrak daun Gaharu (Grynops verstegii) tidak berpengaruh nyata (P0,05) terhadap persentase susut bobot badan broiler, frekuensi denyut jantung, suhu tubuh dan frekuensi pernafasan. Disimpulkan bahwa pemberian ekstrak daun Gaharu sebelum transportasi pada broiler tidak berpengaruh terhadap penurunan stres setelah transportasi hal ini ditunjukkan oleh perubahan suhu tubuh, frekuensi pernafasan, peningkatan denyut jantung dan penyusutan bobot badan yang tidak berbeda nyata (P0.05) dibandingkan dengan kontrol. (The effect of agarwood leaf extract (Grynops versteegii) on transport stress in male broilers) ABSTRAK. This study aims to determine the effect of adding agarwood leaf extract a source of antioxidants before transportation on body weight loss and the physiological conditions of broilers. The materials used were 60 male broilers as samples and agarwood leaf extract obtained through the extraction process using the distillation method. This study used experimental methods with a completely randomized design (CRD) with Duncan's test. The broilers used in the study consisted of 4 treatment groups with different levels of Gaharu leaf extract, they were P0 (control), P1 (250 mg/kg body weight, P2 (300 mg/kg body weight), P3 (350 mg/kg body weight), each treatment was repeated five times and each repetition used 3 individuals. The parameters observed were weight loss, heart rate frequency, increase in body temperature, and respiratory rate. The results showed that the addition of agarwood leaf extract (Grynops verstegii) had no significant effect (P 0.05) on the percentage of broiler body weight loss, heart rate frequency, increased body temperature and respiratory frequency. It was concluded that the addition of agarwood leaf extract before transportation could not reduce body weight loss and the physiological conditions of broilers.

Pengaruh Pemberian Konsentrat Fermentasi dan Silase Eceng Gondok (Eichhornia crassipes) terhadap Konsumsi Pakan, Pertambahan Berat Badan, dan Kimia Darah Domba Ekor Tipis

ABSTRACT. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian konsentrat fermentasi dan silase eceng gondok (Eichhornia crassipes) terhadap konsumsi pakan, pertambahan berat badan, dan kimia darah pada domba ekor tipis. Sebanyak 16 ekor domba ekor tipis digunakan dalam penelitian ini. Rancangan penelitian yang digunakan adalah rancangan acak lengkap pola faktorial terdiri dari 2 faktor yaitu: faktor pakan yang terdiri atas P0 (pakan basal 100%), P1 (pakan basal 80% dan konsentrat fermentasi 20%), P2 (pakan basal 70%, konsentrat fermentasi 10%, dan silase eceng gondok 20%), P3 (pakan basal 60%, konsentrat fermentasi, 10%, dan silase eceng gondok 30%) dan faktor jenis kelamin (jantan dan betina). Parameter penelitian yang diamati adalah konsumsi pakan, pertambahan berat badan, kadar protein, glukosa, dan kolesterol dalam darah. Data yang diperoleh dianalisa dengan analisis sidik ragam, jika diperoleh hasil yang berbeda akan dilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian memperlihatkan perbedaan jenis kelamin berpengaruh sangat nyata (P0,01) terhadap konsumsi pakan, pertambahan berat badan, dan kadar kolesterol darah domba ekor tipis. Perlakuan pakan berpengaruh sangat nyata (P0,01) terhadap pertambahan berat badan, tetapi tidak memberikan pengaruh terhadap kimia darah. Kesimpulannya penggunaan silase eceng gondok sebagai substitusi pakan basal dengan imbangan yang berbeda mampu meningkatkan konsumsi pakan, berat badan harian domba ekor tipis, dan tidak memengaruhi kesehatan ternak jika ditinjau dari komponen kimia darah yang terdiri atas dan kadar glukosa, protein, dan kolesterol darah. (Effect of fermentation concentrate and water hyacinth (Eichhornia crassipes) silage on feed consumption, weight gain, and blood chemistry of thin-tailed sheep) ABSTRAK. This study aimed to determine the effect of fermented concentrate and water hyacinth silage (Eichhornia crassipes) on feed consumption, weight gain, and blood chemistry of thin-tailed sheep. A total of 16 thin tailed-sheep were used in this study. The research design used was a completely randomized design with a factorial pattern consisting of 2 factors, namely: feed factor consisting of P0 (100% basal feed), P1 (80% basal feed and 20% fermented concentrate), P2 (70% basal feed, 10% concentrate fermentation, and 20% water hyacinth silage), P3 (60% basal feed, 10% fermented concentrate, and 30% water hyacinth silage) and sex factors (male and female). The research parameters observed were feed consumption, weight gain, protein, glucose, and cholesterol levels in the blood. The data obtained were analyzed by analysis of variance and continued with Duncan's Multiple Range Test. The results showed that sex differences had a very significant effect (P0.01) on feed consumption, weight gain, and blood cholesterol levels of thin-tailed sheep. Feed treatment had a very significant effect (P0.01) on weight gain but did not affect blood chemistry. In conclusion, the use of water hyacinth silage as a substitute for basal feed was able to increase feed consumption, a daily body weight of thin-tailed sheep, and did not affect livestock health when viewed from the blood chemistry components consisting of glucose, protein, and cholesterol levels.

Kecernaan, Fermentabilitas dan Produksi Protein Mikrobia Secara In Vitro pada Complete Feed Berbasis Pelepah Sawit Fermentasi

ABSTRACT. Penelitian bertujuan mengetahui kualitas complete feed dengan level pelepah sawit fermentasi berdasarkan kecernaan bahan kering, bahan organik, produksi N-NH3, produksi volatile fatty acids (VFA) dan produksi biomassa protein mikrobia serta protein total secara in vitro. Materi yang digunakan adalah complete feed tersusun atas konsentrat dan pelepah sawit fermentasi dengan berbagai level yaitu 0, 10, 20 dan 30%. Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) dengan 4 perlakuan complete feed dengan level pelepah sawit fermentasi yang berbeda. Data diolah menggunakan sidik ragam yang dilanjutkan dengan uji beda wilayah berganda Duncan. Hasil penelitian menunjukkan bahwa complete feed dengan level pelepah sawit fermentasi yang berbeda berpengaruh nyata (p0,05) terhadap kecernaan bahan kering dan bahan organik, produksi N-NH3, produksi VFA, dan produksi protein total, sedangkan pada biomassa protein mikrobia tidak terdapat perbedaan yang nyata (p0,05). Rata-rata nilai kecernaan bahan kering pada perlakuan T0, T1, T2 dan T3 adalah 69,59; 71,9; 69,05; dan 62,58%. Rata-rata nilai kecernaan bahan organik pada perlakuan T0, T1, T2 dan T3 adalah 63,59; 63,15; 65,50; 52,66 %. Rata-rata produksi VFA pada perlakuan T0, T1, T2 dan T3 sebesar 105,8; 142,7; 136,4; dan 135,7 mM. Rata-rata produksi NH3, biomassa protein mikrobia dan produksi protein total pada perlakuan T0, T1, T2 dan T3 berturut-turut adalah 6,48mM, 15,04mg/ml;, 34,10mg/g; 7,36mM, 15,75mg/ml, 23,72mg/g; 8,18mM, 12,59mg/ml, 33,72mg/g); dan 6,60mM, 15,31mg/ml, 40,80mg/g. Berdasarkan hasil penelitian disimpulkan bahwa penggunaan pelepah sawit fermentasi dengan level 20% dalam complete feed menghasilkan produksi VFA, kecernaan bahan kering dan bahan organik yang cukup baik sehingga dapat menjadi pakan alternatif sumber serat pengganti rumput. (Digestibility, fermentability and in-vitro production of microbial protein on complete feed based on fermented palm frond) ABSTRAK. This study aimed to determine the quality of a complete feed containing fermented palm fronds based on the digestibility of dry matter, organic matter, N-NH3, VFA, microbial protein biomass, and total protein in vitro. The material used was complete feed composed of concentrates and fermented palm fronds at various levels, i.e., 0, 10, 20, and 30%. The experiment was conducted as a completely randomized design (CRD) with four complete feed treatments containing different levels of fermented palm fronds. The data were processed using analysis of variance, followed by Duncan’s multiple range test. The results demonstrated that the complete feed with different levels of fermented palm fronds had a significant effect (p0.05) on the digestibility of dry matter and organic matter, N-NH3 production, essential fatty acids production, and total protein production, whereas there was no significant difference (p 0.05) on microbial protein biomass. The average dry matter and organic matter digestibility values of T0, T1, T2, and T3 treatments were 69.59; 63.59, 71.9; 63.15, 69.05; 65.50, and 62.58%; 52.66% respectively. The average production of volatile fatty acids of T0, T1, T2, and T3 treatments were 105.8; 142.7; 136.4; and 135.7 mM. respectively, while the average N-NH3 production, microbial protein biomass, and total protein production of the T0, T1, T2, and T3 treatments were 6.48, 7.36, 8.18, 6.60 mM; 15.04, 75, 12.59, 15.31 mg/ml; and 34.10, 23.72, 33.72, 40.80 mg/g. In conclusion, the use of fermented palm fronds at a 20% level in complete feed gave the best result in the production of volatile fatty acids, improved digestibility of dry matter, and organic matter, so it can be used as an alternative feed to replace grass fiber.

Efek Penambahan Ruminer dalam Ransum Sapi Perah terhadap Produksi 4% FCM dan Nutrien Susu

ABSTRACT. Sapi perah yang berproduksi tinggi membutuhkan ransum berenergi tinggi dan asam lemak esensial. Penambahan lemak dalam ransum dapat mengatasi kekurangan energi dan asam lemak esensial. Permasalahan umum dari pemberian lemak atau minyak pada dosis tertentu dapat mengganggu ekosistem rumen, sehingga dalam pemberiannya perlu diproteksi. Ruminer adalah suplemen komersial berupa minyak sawit yang diproteksi mineral kalsium. Penelitian ditujukan untuk mengetahui penambahan ruminer dalam ransum lengkap terhadap produksi susu 4% FCM dan produksi nutrien susu sapi perah. Penelitian dilakukan secara eksperimental terhadap 10 ekor sapi perah Friesian Holstein pada masa laktasi sama. Sapi tersebut dibagi dua, masing-masing lima ekor. Perlakuan berupa ransum tanpa ditambah ruminer (P0) dan ransum ditambah 250 gram ruminer.ekor-1.hari-1 (P1). Peubah yang diamati adalah produksi susu 4% FCM diukur setiap hari selama 60 hari dan kualitas susu diukur setiap 10 hari. Hasil penelitian menunjukkan bahwa produksi susu 4% FCM, produksi total padatan, lemak, protein dan laktosa susu pada sapi diberi ruminer lebih tinggi (P0,05) daripada sapi yang tidak diberi ruminer. Disimpulkan bahwa pemberian ruminer meningkatkan produksi susu 4% FCM, total padatan, lemak, protein dan laktosa susu. Pemberian ruminer sebanyak 250 gram.ekor-1hari-1 mampu meningkatkan 2 kg produksi susu 4% FCM pada sapi perah laktasi. (Effects of ruminer addition in dairy cattle rations on milk 4% FCM and nutrient production) ABSTRAK. High-yielding dairy cows require high-energy rations and essential fatty acids. The addition of fat in the ration can overcome the lack of energy and essential fatty acids. The general problem of giving fat or oil at certain doses can disrupt the rumen ecosystem, so that it needs to be protected. Ruminer is a commercial supplement in the form of calcium mineral protected palm oil. This research was aimed to determine the addition of ruminants in complete rations on the production of 4% FCM milk and the nutrient production of dairy cow milk. The research was carried out experimentally on 10 dairy cows during the same lactation period. The cow is divided into two, five each. The treatments were rations without adding ruminer (P0) and rations added by 250 grams of ruminer. head-1 day-1 (P1). The variables observed were 4% FCM milk production measured every day for 60 days and milk quality was measured every 10 days. The results showed that the production of 4% FCM milk, the total production of solids, fat, protein and milk lactose in cows fed with ruminer was higher (P 0.05) than cows that were not given ruminer. It was concluded that the provision of ruminer increased milk production by 4% FCM, total solids, fat, protein and milk lactose. Giving a ruminer of 250 grams of head-1.day-1 was able to increase 2 kg of 4% FCM milk production in lactating dairy cows.

Respon Superovulasi Sapi Persilangan Belgian Blue dengan Metode yang Berbeda

ABSTRACT. Superovulasi merupakan suatu metode pemanfaatan induk betina unggul untuk menghasilkan embrio sebanyak-banyaknya untuk kegiatan transfer embrio. Tujuan penelitian ini membandingkan 2 teknik superovulasi yang berbeda pada sapi persilangan Belgian Blue. Penelitian dilakukan di Balai Embrio Ternak Cipelang Bogor dengan menggunakan 24 ekor sapi persilangan Belgian Blue dengan umur 2-3 tahun, masing-masing perlakuan 12 ekor. Penelitian dilakukan secara eksperimen dengan dua perlakuan, P1 = Superovulasi menggunakan penyuntikan FSH selama 3 hari pada pagi dan sore (metode konvensional) dengan dosis 400 mg FSH dalam 20 ml pelarut dan P2 = Superovulasi menggunakan penyuntikan tunggal (satu kali) FSH dengan dosis 400 mg FSH dalam 3 ml pelarut . Parameter pada penelitian ini adalah Jumlah Corpus luteum (CL), respon rate, perolehan embrio, kualitas embrio, recovery rate, dan tingkat fertilisasi. Data yang diperoleh diuji dengan uji T tidak berpasangan. Hasil analisa data menunjukkan hasil yang tidak berbeda nyata (P0,05) pada jumlah CL P1 : 8,42+3,06 dan P2 : 6,08+4,74; Respon rate P1 : 100% dan P2 : 75%; Total perolehan embrio P1 : 6,08+2,64 embrio dan P2 : 4,45+4,01 embrio; Embrio recovery rate P1 : 70,37+9,18% dan P2 : 61,33+12,12%; Embrio Layak Transfer P1 : 3,83+2,92 embrio dan P2 : 2,73+2,28 embrio; dan berbeda nyata (P0,05) pada rataan fertilisasi P1 : 79,10% dan P2 : 95,26%. Kesimpulan penelitian ini adalah teknik superovulasi penyuntikan tunggal FSH secara subkutan memberikan efek superovulasi dan menghasilkan embrio dengan jumlah dan kualitas yang tidak berbeda nyata dengan teknik superovulasi konvensional. (Superovulation Responses of Belgian Blue Crossbreed Cattle Treated with Different Superovulation Methods) ABSTRAK. Superovulation is a technique for producing a large number of embryos for embryo transfer using a genetically superior female. The purpose of this research was to compare two alternative methods of superovulation in Belgian Blue crossbreed cattle. The study used 24 Belgian Blue crossbred cattle aged 2-3 years, including 12 cows per treatment, at the National Livestock Embryo Center of Cipelang in Bogor. The research was done in an experimental setting using two different treatments, P1 = Superovulation using twice daily FSH injections for three days at a dose of 400 mg FSH dissolved in 20 ml of saline, and P2 = Superovulation using FSH single injections at a dose of 400 mg FSH dissolved in 3 ml of saline. The parameters in this study were the number of corpus luteum (CL), response rate, total number of embryo/ova collection, embryo quality, recovery rate, and fertilization rate. The data obtained were tested by unpaired T test. The results of data analysis showed results that were not significantly different (P0.05) on the number of CL P1 : 8.42+3.06 and P2 : 6.08+4.74; Response rate P1 : 100% and P2 : 75%; Total number of embryos collection P1 : 6.08+2.64 embryos and P2 : 4.45+4.01 embryos; Embryo recovery rate P1 : 70.37+9.18% and P2 : 61.33+12.12%; Transferable Embryos P1 : 3.83+2.92 embryos and P2 : 2.73+2.28 embryos; and significantly different (P0.05) on fertilization rate P1 : 79.10% and P2 : 95.26%. The conclusion of this study show that the subcutaneous FSH single injection technique induces superovulation and produces embryos that are similar in number and quality to those produced by conventional superovulation techniques.

Evaluasi Status Reproduksi Sapi Hasil Persilangan Peranakan Ongole dengan Bali

ABSTRACT. Tujuan penelitian untuk mengetahui status reproduksi hasil persilangan sapi peranakan ongole (PO) dan sapi bali (yang selanjutnya dinamakan sapi POBA) jantan dan betina melalui beberapa kegiatan lapang. Penelitian ini bersifat non eksperimental yang dilakukan secara eksploratif; menggunakan 20 ekor sapi berumur antara 32 sampai 36 bulan. Parameter yang diamati meliputi kualitas semen, kondisi organ reproduksi sapi betina dan angka kebuntingan. Hasil penelitian menunjukkan, kualitas semen sapi POBA rendah atau jelek berdasarkan hasil analisis secara mikroskopis maupun makroskopis; namun memiliki libido yang cukup tinggi yaitu rata-rata sekitar 4 menit 25 detik dari mulai mengendus atau didekatkan dengan ternak pemancing (teaser) sampai ejakulasi. Secara umum kondisi organ reproduksi sapi POBA betina normal, sehingga siap dijadikan indukan yang produktif. Sebanyak 66,67% menunjukkan perilaku estrus yang tampak terutama keluar lendir yang terlihat jelas menggantung di bibir vagina bahkan diantaranya mengejar dan/atau menaiki sapi-sapi lain. Namun hasil pemeriksaan kebuntingan (PKB) melalui palpasi rektal menunjukkan tidak ada sapi betina yang bunting. Dapat disimpulkan bahwa sapi POBA jantan infertil, yang diindikasikan melalui hasil pemeriksaan semen dan tidak adanya sapi betina yang berhasil bunting melalui perkawinan alam selama 3 (tiga) bulan. Sedangkan sapi betina POBA memiliki organ reproduksi normal serta menunjukkan ekspresi estrus yang baik. (The evaluation of cattle reproduction status between crosses Ongole Crossbreed with Bali cows) ABSTRAK. The purpose of this research was to determine the reproductive status of the crossbred PO and Bali cattle (hereinafter referred to as POBA cattle) through several field activities. This research is non-experimental and carried out exploratory using 20 cattle aged 32 to 36 months. Parameters observed included semen quality, cows' reproductive organ condition, and pregnancy rate. The quality of POBA bulls semen was low or bad as indicated by the results of microscopic and macroscopic analysis; but had pretty high libido, which was an average of about 4 minutes and 25 seconds, starting to sniff or being brought near to teaser until ejaculation. In general, the reproductive organs of POBA cows were normal so that they were ready to become productive sires. As many as 66.67% showed estrus behavior, especially visible mucus that was hanging on the lips of the vagina, even among them chasing and/or riding other cattle. However, the results of pregnancy examination through rectal palpation showed that there were no pregnant cows. It can be concluded that POBA bulls are infertile, which was indicated by the results of semen examination and the absence of successfully conceived cows through natural mating for 3 (three) months. Meanwhile, POBA cows have normal reproductive organs and show good estrus expression.