Tissue Tropism of Three Cloacal Isolates and Massachusetts Strain of Infectious Bronchitis Virus

1990 ◽  
Vol 34 (4) ◽  
pp. 865 ◽  
Author(s):  
Benjamin Lucio ◽  
Julius Fabricant
Keyword(s):  
Infectious Bronchitis Virus ◽  
Tissue Tropism ◽  
Infectious Bronchitis

scholarly journals Pathogenicity and tissue tropism of infectious bronchitis virus is associated with elevated apoptosis and innate immune responses

Virology ◽  
2016 ◽  
Vol 488 ◽  
pp. 232-241 ◽  
Author(s):  
Rajesh Chhabra ◽  
Suresh V Kuchipudi ◽  
Julian Chantrey ◽  
Kannan Ganapathy
Keyword(s):  
Immune Responses ◽  
Infectious Bronchitis Virus ◽  
Innate Immune ◽  
Tissue Tropism ◽  
Innate Immune Responses ◽  
Infectious Bronchitis

scholarly journals A highly pathogenic GI-19 lineage infectious bronchitis virus originated from multiple recombination events with broad tissue tropism

2020 ◽  
Vol 285 ◽  
pp. 198002 ◽  
Author(s):  
Yutong Hou ◽  
Lili Zhang ◽  
Mengting Ren ◽  
Zongxi Han ◽  
Junfeng Sun ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Tissue Tropism ◽  
Highly Pathogenic ◽  
Infectious Bronchitis ◽  
Multiple Recombination

REDUCED CHICKEN EMBRYO DWARFING EFFECT IS RELATED TO INFECTIOUS BRONCHITIS VIRUS TW2575/98 REPLICATION EFFICIENCY

2020 ◽  
Vol 46 (02n03) ◽  
pp. 85-93
Author(s):  
Cheng-Ta Tsai ◽  
Ming-Chang Lee ◽  
Ching-Ho Wang
Keyword(s):  
Vaccine Strain ◽  
Infectious Bronchitis Virus ◽  
Tissue Tropism ◽  
Renal Tubules ◽  
Pathological Changes ◽  
In Ovo ◽  
Chicken Embryos ◽  
Infectious Bronchitis ◽  
Attenuated Vaccine ◽  
Embryonic Death

An attenuated infectious bronchitis virus (TW2575/98) vaccine strain was successfully developed after 75 serial passages in embryonated chicken eggs. However, the in ovo vaccination for disease control was not applied in practice because this vaccine strain is highly pathogenic to chicken embryos (CEs) causing early death, dwarfing and other harmful effects. We compared the differences in virus replication, pathological changes, and tissue tropism between the wild virus and attenuated vaccine strain in CEs inoculated with different viral titer levels, i.e. 0.1, 1 and 10 EID[Formula: see text]/egg. The wild virus caused dwarfing effect at high titer inoculation, whereas the attenuated vaccine strain caused the dwarfing effect only at a lower viral inoculation accompanied by the earlier infection establishment and embryonic death at high and medium titers. There were no significant differences in the pathological changes in CEs infected by both wild and attenuated strains. Detected by immunohistochemistry, the viral antigens of both strains could be found mainly at the epithelium of the chorioallantoic membrane, lung parabronchus, renal tubules and some in the spleen and heart serosa. These findings indicated that the early embryonic death and dwarfing is not related to the change in cell/tissue tropism of the vaccine strain, rather on the early infection establishment and viral load. We suggest that the vaccine strain inoculated titer could be adjusted to an optimal low level for in ovo vaccination to overcome the poor hatching rate for its higher virulence to chicken embryos.

scholarly journals Experimental study on histopathological changes and tissue tropism of Iranian infectious bronchitis serotype 793/B-like virus in SPF chickens

2013 ◽  
Vol 84 (1) ◽  
Author(s):  
Peyman Bijanzad ◽  
Reza Momayez ◽  
Mohammad H. Bozorgmehrifard ◽  
Mohammad H. Hablolvarid ◽  
Seyed A. Pourbakhsh
Keyword(s):  
Infectious Bronchitis Virus ◽  
Bursa Of Fabricius ◽  
Control Group ◽  
Lymphoid Tissues ◽  
Tissue Tropism ◽  
Post Inoculation ◽  
Avian Infectious Bronchitis Virus ◽  
Histopathological Changes ◽  
Infectious Bronchitis ◽  
Histopathological Lesions

Avian infectious bronchitis virus (IBV) is prevalent in all countries with intensive poultry flocks. This disease is characterised primarily by respiratory signs, but some IBV strains may also infect other organs such as the intestinal and urogenital tracts. The aim of this study was to characterise the histopathological lesions and tissue tropism of Iranian isolate IR/773/2001(793/B) of avian infectious bronchitis virus in different organs of experimentally infected SPF chickens. Forty-two one-day-old, specific pathogen-free (SPF) chicks were divided randomly into two groups (21 chicks to each group). At the age of 12 days, one group was inoculated intra-ocularly with 103 EID50 of the 793/B isolate, and the other was kept as the control group. Tissue samples were collected at 2, 4, 6, 8, 10 and 12 days post-inoculation (PI). The IBV virus was detected in the caecal tonsils and cloaca from the 2nd to the 12th day PI. The virus was also detected in the kidneys from days 4–10 PI and in the bursa of Fabricius from days 4–12 PI. The virus was detected in the trachea, lungs and thymus. The most obvious histopathological lesions were found in the trachea, kidney, lungs and bursa of Fabricius. Amongst the lymphoid tissues, histopathological changes were found most frequently in the bursa of Fabricius. The results of this study indicated that the 793/B serotype of IBV is unlikely to cause mortality, severe clinical signs or gross lesions in infected chickens, but its replication in some tissues including the bursa of Fabricius could render birds susceptible to other micro-organisms.

scholarly journals Tissue Distribution of Avian Infectious Bronchitis Virus following in Ovo Inoculation of Chicken Embryos Examined by in Situ Hybridization with Antisense Digoxigenin-Labeled Universal Riboprobe

2002 ◽  
Vol 14 (5) ◽  
pp. 377-381 ◽  
Author(s):  
Chang-Won Lee ◽  
Corrie Brown ◽  
Mark W. Jackwood
Keyword(s):  
In Situ Hybridization ◽  
Infectious Bronchitis Virus ◽  
Tissue Tropism ◽  
Positive Staining ◽  
In Ovo ◽  
Chicken Embryos ◽  
Infectious Bronchitis ◽  
Different Strains

Chicken embryos were inoculated with 8 different strains of infectious bronchitis virus (IBV) representing 7 different serotypes at 17 days of embryonation. At 2 and 5 days postinfection (dpi), tissues were collected for in situ hybridization using an antisense digoxigenin-labeled riboprobe corresponding to the sequence of the mRNA coding for the membrane protein. Extensive antigen staining in the cytoplasm of epithelial cells in the trachea, lung, bursa, and intestine was detected at 2 dpi with all 8 strains of IBV. At 5 dpi, little or no positive staining was observed in these tissues. However, tubular cells of the kidney showed multifocal positive staining with the Wolgemuth strain-, Gray strain-, JMK strain-, and Mass41 strain-infected chickens. No viral RNA was detected in the spleen at any time point. The results demonstrated strict epitheliotropic nature and wide tissue tropism of strains of IBV in the chicken embryo and the universality of our riboprobe. In situ hybridization with this probe will be useful for understanding the tissue tropism and the pathogenesis of IBV in vivo.

scholarly journals Molecular Biology and Pathological Process of an Infectious Bronchitis Virus with Enteric Tropism in Commercial Broilers

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1477
Author(s):  
Ana P. da Silva ◽  
Ruediger Hauck ◽  
Sabrina R. C. Nociti ◽  
Colin Kern ◽  
H. L. Shivaprasad ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Molecular Mechanisms ◽  
Phylogenetic Analyses ◽  
Nucleotide Composition ◽  
Pathological Process ◽  
Tissue Tropism ◽  
Full Genome ◽  
Infectious Bronchitis ◽  
Viral Shedding ◽  
Intestinal Lesions

Infectious bronchitis virus (IBV) induces respiratory and urogenital disease in chickens. Although IBV replicates in the gastrointestinal tract, enteric lesions are uncommon. We have reported a case of runting-stunting syndrome in commercial broilers from which an IBV variant was isolated from the intestines. The isolate, CalEnt, demonstrated an enteric tissue tropism in chicken embryos and SPF chickens experimentally. Here, we determined the full genome of CalEnt and compared it to other IBV strains, in addition to comparing the pathobiology of CalEnt and M41 in commercial broilers. Despite the high whole-genome identity to other IBV strains, CalEnt is rather unique in its nucleotide composition. The S gene phylogenetic analyses showed great similarity between CalEnt and Cal 99. Clinically, vent staining was slightly more frequent in CalEnt-infected birds than those challenged with M41. Furthermore, IBV IHC detection was more evident and the viral shedding in feces was overall higher with the CalEnt challenge compared with M41. Despite underlying intestinal lesions caused by coccidiosis and salmonellosis vaccination, microscopic lesions in CalEnt-infected chickens were more severe than in M41-infected chickens or controls, supporting the enteric tropism of CalEnt. Further studies in SPF chickens are needed to determine the pathogenesis of the virus, its molecular mechanisms for the enteric tropism, and its influence in intestinal health.

Ultrastructure and biological properties of avian infectious bronchitis virus following cryopreservation

2015 ◽  
Vol 25 (4) ◽  
pp. 340-349 ◽  
Author(s):  
Marina Yu. Stegniy ◽  
◽  
Boris T. Stegniy ◽  
Anatoliy N. Goltsev ◽  
◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Biological Properties ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis
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