Development of a Quantitative-Competitive Polymerase Chain Reaction Assay for Serotype 1 Marek's Disease Virus

A total of 24 clinical specimens (10 feathers, 8 peripheral bloods and 6 spleens) were collected from 10 suspected outbreaks of Marek’s disease (MD) from Mymensingh, Tangail, Gazipur and Pabna districts of Bangladesh. A polymerase chain reaction (PCR) protocol originally described by Silva (1992) was adopted to detect Marek’s disease virus (MDV) genome in these specimens. All the tested peripheral blood buffy coat samples (100%) were positive for MDV in PCR, while 70% of feather samples and 66.6% of spleen samples were positive. A band of 317 bp size was found in all positive samples. A few samples also yielded additional bands of 185 bp size and/or multiple bands of larger than 317 bp size, indicating the presence of both virulent MDV and the vaccine virus. The study suggests that peripheral blood and feathers from live birds, and feathers from dead birds are the samples of choice for the detection of MDV by PCR. The Bangladesh Veterinarian (2018) 35(1&2): 1-6

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Quantification of Marek's disease virus in chicken lymphocytes using the polymerase chain reaction with fluorescence detection

Journal of Virological Methods ◽

10.1016/s0166-0934(96)02172-6 ◽

1997 ◽

Vol 65 (1) ◽

pp. 75-81 ◽

Cited By ~ 42

Author(s):

Nat Bumstead ◽

Julie Sillibourne ◽

Mike Rennie ◽

Norman Ross ◽

Fred Davison

Keyword(s):

Polymerase Chain Reaction ◽

Disease Virus ◽

Fluorescence Detection ◽

Marek's Disease Virus ◽

Marek's Disease ◽

Marek’S Disease Virus ◽

Marek’S Disease ◽

Chain Reaction ◽

Polymerase Chain

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Use of the polymerase chain reaction for the diagnosis of natural infection of chickens and turkeys with Marek's disease virus and reticuloendotheliosis virus

Avian Pathology ◽

10.1080/03079459508419050 ◽

1995 ◽

Vol 24 (1) ◽

pp. 69-94 ◽

Cited By ~ 54

Author(s):

Irit Davidson ◽

Anya Borovskaya ◽

S. Perl ◽

M. Malkinson

Keyword(s):

Polymerase Chain Reaction ◽

Disease Virus ◽

Natural Infection ◽

Marek's Disease Virus ◽

Marek's Disease ◽

Marek’S Disease Virus ◽

Marek’S Disease ◽

Reticuloendotheliosis Virus ◽

Chain Reaction ◽

Polymerase Chain

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Development of a Nested Polymerase Chain Reaction Method to Detect Oncogenic Marek's Disease Virus from Feather Tips

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063870701900503 ◽

2007 ◽

Vol 19 (5) ◽

pp. 471-478 ◽

Cited By ~ 9

Author(s):

Shiro Murata ◽

Kyung-Soo Chang ◽

Sung-Il Lee ◽

Satoru Konnai ◽

Misao Onuma ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Disease Virus ◽

Marek's Disease Virus ◽

Marek's Disease ◽

Marek’S Disease Virus ◽

Chain Reaction ◽

Nested Polymerase Chain Reaction ◽

Polymerase Chain ◽

Highly Virulent ◽

Meq Gene

For the easy survey of Marek's disease virus (MDV), feather tip–derived DNA from MDV-infected chickens can be used because feather tips are easy to collect and feather follicle epithelium is known to be the only site of productive replication of cell-free MDV. To develop a diagnostic method to differentiate highly virulent strains of MDV from the attenuated MDV vaccine strain, CVI988, which is widely used, nested polymerase chain reaction (PCR) was performed to detect a segment of the meq gene in feather tip samples of chickens experimentally infected with MDV. In chickens infected with Md5, a strain of oncogenic MDV, the meq gene was consistently detected, whereas the L- meq gene, in which a 180–base pair (180-bp) sequence is inserted into the meq gene, was detected in CVI988-infected chickens. Moreover, the meq gene was mainly detected even in chickens co-infected with both Md5 and CVI988. These results suggest that this method is appropriate for the surveillance of the highly virulent MDV infection in the field.

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