Genetic Variability and Population Differentiation Inferred from DNA Fingerprinting in Silvereyes (Aves: Zosteropidae)

Evolution ◽  
1993 ◽  
Vol 47 (4) ◽  
pp. 1105 ◽  
Author(s):  
Sandie M. Degnan
2020 ◽  
Vol 49 (6) ◽  
pp. 1083-1092
Author(s):  
S Goitom ◽  
M.G. Gicheha ◽  
F.K. Njonge ◽  
N Kiplangat

Indigenous cattle play a vital role in subsistence and livelihood of pastoral producers in Eritrea. In order to optimally utilize and conserve these valuable indigenous cattle genetic resources, the need to carry out an inventory of their genetic diversity was recognized. This study assessed the genetic variability, population structure and admixture of the indigenous cattle populations (ICPs) of Eritrea using a genotype by sequencing (GBS) approach. The authors genotyped 188 animals, which were sampled from 27 cattle populations in three diverse agro-ecological zones (western lowlands, highlands and eastern lowlands). The genome-wide analysis results from this study revealed genetic diversity, population structure and admixture among the ICPs. Averages of the minor allele frequency (AF), observed heterozygosity (HO), expected heterozygosity (HE), and inbreeding coefficient (FIS) were 0.157, 0.255, 0.218, and -0.089, respectively. Nei’s genetic distance (Ds) between populations ranged from 0.24 to 0.27. Mean population differentiation (FST) ranged from 0.01 to 0.30. Analysis of molecular variance revealed high genetic variation between the populations. Principal component analysis and the distance-based unweighted pair group method and arithmetic mean analyses revealed weak substructure among the populations, separating them into three genetic clusters. However, multi-locus clustering had the lowest cross-validation error when two genetically distinct groups were modelled. This information about genetic diversity and population structure of Eritrean ICPs provided a basis for establishing their conservation and genetic improvement programmes. Keywords: genetic variability, molecular characterization, population differentiation


2002 ◽  
Vol 62 (3) ◽  
pp. 503-508 ◽  
Author(s):  
N. MORAES ◽  
J. S. MORGANTE ◽  
C. Y. MIYAKI

In this study we analyzed a population of Bradypus torquatus with individuals originally distributed in different localities of Bahia, and two populations of B. variegatus with individuals from Bahia and São Paulo States. Using the DNA fingerprinting method, we assessed the genetic variability within and between populations. Analysis of the DNA profiles revealed genetic similarity indices ranging from 0.34 ± 0.07 to 0.87 ± 0.04. Similar low levels of genetic variability were found only in isolated mammalian populations or among related individuals. This study presents the first analyses of genetic diversity in sloth populations.


1995 ◽  
Vol 91-91 (6-7) ◽  
pp. 856-861 ◽  
Author(s):  
J. G. Sastry ◽  
W. Ramakrishna ◽  
S. Sivaramakrishnan ◽  
R. P. Thakur ◽  
V. S. Gupta ◽  
...  

1994 ◽  
Vol 81 (1) ◽  
pp. 34-36
Author(s):  
J. Heinze ◽  
J. Gadau ◽  
B. H�lldobler ◽  
I. Nanda ◽  
M. Schmid ◽  
...  

Genome ◽  
1992 ◽  
Vol 35 (4) ◽  
pp. 681-688 ◽  
Author(s):  
Alain Blanchetot

The most conventional approach for evaluating genetic variability in an insect population involves assessing the degree of enzyme polymorphism. Hymenoptera display a relatively low level of genetic variability compared with most insect species. DNA probes consisting of tandemly repeated sequences are powerful tools for detecting polymorphisms when employed to develop DNA fingerprinting (DNAfp) profiles in a wide range of organisms. This report describes genetic variability in the solitary bee species Megachile rotundata as assessed by DNAfp using the Ml3 sequence and a synthetic oligonucleotide sequence homologous to a hypervariable region of the α-globin gene. DNAfp comparisons among offspring were used to analyze genealogical structure in M. rotundata nests. The results indicate that polyandry, by a large number of males, is not a common phenomenon in M. rotundata bee species. In the present analysis, it is likely that the broods raised in single nests are mostly the offspring of one singly mated female. However, the data does not preclude that for certain nests two males could have been involved in the mating process.Key words: Megachile rotundata, DNA fingerprinting, M13 sequence, α-globin hypervariable sequence, intra-nest genetic relationships.


Sign in / Sign up

Export Citation Format

Share Document