The Effect of Copper, Zinc, Cobalt and Manganese on the Growth of the Marine Diatom Nitzschia closterium

Cell surface hydropobicity plays a significant role in microbe-mineral interactions with special relevance of bioleaching. The present investigation envisages a study on the hydrophobic character of Acidiphilium symbioticum KM2, an acidophilic strain of bioleaching environment, when grown in presence of heavy metals - copper, zinc, cadmium and nickel. The metals, at its sub inhibitory concentrations (MIC50), exhibited profound negative effect on the growth of the bacterium. Inhibition on the culture growth rate was highest due to cadmium followed by zinc, nickel and copper. However, upon successive adaptation in different concentrations of each metal in ascending order, the cells could grow rapidly in the presence of higher concentrations of the metals indicating good metal resistance by the bacterium. Compared to normally grown cells, A. symbioticum KM2, when grown in presence of the metals, became more hydrophobic, which was dependent on the metal and its concentration in the media. Among the four metals studied, the effect of copper was found to be the highest, where up to 74 % increase in the bacterial cell hydrophobicity was observed.

Download Full-text

EFFECT OF COPPER ON GROWTH, SILICIC ACID UPTAKE AND SOLUBLE POOLS OF SILICIC ACID IN THE MARINE DIATOM, THALASSIOSIRA WEISFLOGII (BACILLARIOPHYCEAE)1

Journal of Phycology ◽

10.1111/j.0022-3646.1983.00101.x ◽

1983 ◽

Vol 19 (1) ◽

pp. 101-104 ◽

Cited By ~ 4

Author(s):

John G. Rueter

Keyword(s):

Silicic Acid ◽

Marine Diatom ◽

Acid Uptake ◽

Effect Of Copper

Download Full-text

Interactions of copper and manganese: A mechanism by which manganese alleviates copper toxicity to the marine diatom, Nitzschia closterium (Ehrenberg) W. Smith

Aquatic Toxicology ◽

10.1016/0166-445x(85)90042-6 ◽

1985 ◽

Vol 7 (4) ◽

pp. 241-254 ◽

Cited By ~ 30

Author(s):

J.L. Stauber ◽

T.M. Florence

Keyword(s):

Copper Toxicity ◽

Marine Diatom ◽

Nitzschia Closterium

Download Full-text

Effect of Copper Acyclovir Complexes on Herpes Simplex Virus Type 1 and Type 2 (HSV-1, HSV-2) Infection in Cultured Cells

Metal-Based Drugs ◽

10.1155/mbd.1998.19 ◽

1998 ◽

Vol 5 (1) ◽

pp. 19-23 ◽

Cited By ~ 4

Author(s):

M. Panteva ◽

T. Varadinova ◽

I. Turel

Keyword(s):

Cultured Cells ◽

Sensitive Strain ◽

Published Data ◽

Relative Level ◽

Mdbk Cells ◽

Copper Zinc ◽

Effect Of Copper ◽

Simplex Virus ◽

Hsv 1

We have found that when copper, zinc or cobalt is bound to a suitable ligand, the appropriate complex exhibited a significant anti-HSV effect (Varadinova et al., 1993; 1996). Recently published data by Sagripanti et al. (1997) also show that the inhibition of HSV by copper was enhanced by reducing agents and that mechanism of the inactivation is similar as for copper-mediated DNA damage (Aruoma, et al. 1991; Dizdaroglu, et al., 1991; Toyokuni and Sagripanti, 1994). Therefore it was interesting to study the efect of Cu(ll) coordination compounds with acyclovir (ACV) on the replication of HSV in cultured cells. The experiments on cytotoxicity as well as on the activity of three different Cu-ACV complexes [Cu(ACV)2Cl2(H2O)2] = (A); [Cu(ACV)2(H2O)3](NO3)2.H2O = (B) and [Cu(ACV)2(H2O)2](NO3)2] = (C) towards virus replication, with special attention on the growth of ACV-resistant strain R-100 were performed on MDBK cells. ACV was used as a reference compound. The following results were obtained: 1) Increased cell’s viability in the presence of 20-40(g/ml ACV and decreased one in the presence of Cu-ACV complexes with relative level (A) >> (B) > (C); 2) Cu-ACV complexes are more cytotoxic than the ligand - ACV and the relative level is (C)>(B)>(A); 3) The anti-HSV effect of ACV can be modulated by copper at levels depending on the specificity of the particular virus strain: (i) for the ACV sensitive strain DA (HSV-1) - ACV ((A) > (C) > (B); (ii) for the ACV sensitive strain Bja (HSV-2) (A) > ACV > (C) > (B); (iii) for strain R-100 ( ACVR, TKa) - (A) > ACV > (C) > (B). This findings are consistent with previously published data and undoubtedly show that Cu-ACV complexes could be useful in the treatment of HSV infections, especially when the causative agent is a resistant to ACV mutant.

Download Full-text