Studies on the Effects of Radiation on Living Cells in Tissue Culture: II. Radiosensitivity of Cells Isolated from Various Imaginal Discs and Organs of Larvae of Drosophila melanogaster

1960 ◽  
Vol 13 (6) ◽  
pp. 825 ◽  
Author(s):  
Masakatsu Horikawa ◽  
Tsutomu Sugahara
Keyword(s):  
Tissue Culture ◽  
Drosophila Melanogaster ◽  
Imaginal Discs ◽  
Living Cells ◽  
Effects Of Radiation

scholarly journals Imaginal discs regulate developmental timing in Drosophila melanogaster

2008 ◽  
Vol 321 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Bradley C. Stieper ◽  
Mania Kupershtok ◽  
Michael V. Driscoll ◽  
Alexander W. Shingleton
Keyword(s):  
Drosophila Melanogaster ◽  
Imaginal Discs ◽  
Developmental Timing

Comparative Analysis of Spatial Patterns of Gene Expression in Drosophila melanogaster Imaginal Discs

2007 ◽  
pp. 533-547 ◽  
Author(s):  
Cyrus L. Harmon ◽  
Parvez Ahammad ◽  
Ann Hammonds ◽  
Richard Weiszmann ◽  
Susan E. Celniker ◽  
...  
Keyword(s):  
Gene Expression ◽  
Drosophila Melanogaster ◽  
Comparative Analysis ◽  
Spatial Patterns ◽  
Imaginal Discs

scholarly journals DNA replication of histone gene repeats in Drosophila melanogaster tissue culture cells: multiple initiation sites and replication pause sites.

1993 ◽  
Vol 13 (7) ◽  
pp. 4098-4106 ◽  
Author(s):  
T Shinomiya ◽  
S Ina
Keyword(s):  
Tissue Culture ◽  
Drosophila Melanogaster ◽  
Dna Replication ◽  
Present Report ◽  
Polymerase Chain Reaction Method ◽  
Mapping Method ◽  
Histone Gene ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Replication Intermediates

We showed previously that DNA replication initiates at multiple sites in the 5-kb histone gene repeating unit in early embryos of Drosophila melanogaster. The present report shows evidence that replication in the same chromosomal region initiates at multiple sites in tissue culture cells as well. First, we analyzed replication intermediates by the two-dimensional gel electrophoretic replicon mapping method and detected bubble-form replication intermediates for all fragments restricted at different sites in the repeating unit. Second, we analyzed bromodeoxyuridine-labeled nascent strands amplified by the polymerase chain reaction method and detected little differences in the size distribution of nascent strands specific to six short segments located at different sites in the repeating unit. These results strongly suggest that DNA replication initiates at multiple sites located within the repeating unit. We also found several replication pause sites located at 5' upstream regions of some histone genes.

Heterogeneous nuclear ribonucleoprotein complexes and proteins in Drosophila melanogaster

1992 ◽  
Vol 12 (2) ◽  
pp. 847-855
Author(s):  
G Raychaudhuri ◽  
S R Haynes ◽  
A L Beyer
Keyword(s):  
Tissue Culture ◽  
Drosophila Melanogaster ◽  
Rna Sequences ◽  
Heterogeneous Nuclear Ribonucleoprotein ◽  
Culture Cells ◽  
Ribonucleoprotein Complexes ◽  
Tissue Culture Cells ◽  
Hnrnp Proteins ◽  
Nuclear Ribonucleoprotein ◽  
Basic Region

Pre-mRNAs cotranscriptionally associate with a small group of proteins to form heterogeneous nuclear ribonucleoprotein (hnRNP) complexes. We have previously identified two genes in Drosophila melanogaster, Hrb98DE and Hrb87F (i.e., genes at 98DE and 87F encoding putative hnRNA binding proteins), which encode five protein species homologous to the mammalian A-B hnRNP proteins. The studies presented herein show that antibodies against the RNP domains of Hrb98DE reacted with 10 to 15 distinct spots of 38 to 40 kDa in the basic region of two-dimensional gels. These nuclear proteins bound single-stranded nucleic acids and were extracted from Drosophila tissue culture cells as 40 to 80S hnRNP complexes in association with 300 to 800 nucleotide fragments of RNA. The peak of poly(A)+ RNA sequences was coincident with the peak of HRB proteins in sucrose gradients, strongly suggesting that the HRB complexes identified are Drosophila hnRNP complexes. The repertoire of HRB proteins did not change significantly during embryogenesis and was similar to that observed in Drosophila tissue culture cells. Analyses with peptide-specific antisera demonstrated that the major proteins in the hnRNP complex were encoded by the two genes previously identified. Although the Drosophila HRB proteins are only approximately 60% identical throughout the RNP domains to the mammalian A-B hnRNP proteins, features of the basic pre-mRNA packaging mechanism appear to be highly conserved between D. melanogaster and mammals.

p75, a polypeptide component of karyoskeletal protein-enriched fractions associated with transcriptionally active loci of Drosophila melanogaster polytene chromosomes

1988 ◽  
Vol 8 (5) ◽  
pp. 1877-1886
Author(s):  
B M Benton ◽  
S Berrios ◽  
P A Fisher
Keyword(s):  
Tissue Culture ◽  
Drosophila Melanogaster ◽  
Transcriptional Regulation ◽  
Salivary Gland ◽  
Indirect Immunofluorescence ◽  
Polytene Chromosomes ◽  
Nuclear Interior ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Larval Salivary Gland

A 75-kilodalton polypeptide has been identified which copurifies with karyoskeletal protein-enriched fractions prepared from Drosophila melanogaster embryos. Results of indirect immunofluorescence experiments suggest that this protein, here designated p75, is primarily associated with puffed regions of larval salivary gland polytene chromosomes. In nonpolytenized Schneider 2 tissue culture cells, p75 appeared to be localized throughout the nuclear interior during interphase. In mitotic cells, p75 was redistributed diffusely. A possible role for karyoskeletal elements in transcriptional regulation is discussed.

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