Comparative Analysis of Spatial Patterns of Gene Expression in Drosophila melanogaster Imaginal Discs

Author(s):  
Cyrus L. Harmon ◽  
Parvez Ahammad ◽  
Ann Hammonds ◽  
Richard Weiszmann ◽  
Susan E. Celniker ◽  
...  
Genetics ◽  
1991 ◽  
Vol 129 (2) ◽  
pp. 443-461 ◽  
Author(s):  
A M Pattatucci ◽  
T C Kaufman

Abstract The Sex combs reduced (Scr) locus is unique among the genes contained within the Antennapedia complex (ANT-C) of Drosophila melanogaster in that it directs functions that are required for both cephalic and thoracic development in the embryo and the adult. Antibodies raised against protein encoded by Scr were used to follow the distribution of this gene product in embryos and imaginal discs of third instar larvae. Analysis of Scr protein accumulation in embryos hemizygous for breakpoint lesions mapping throughout the locus has allowed us to determine that sequences required for establishment of the Scr embryonic pattern are contained within a region of DNA that overlaps with the identified upstream regulatory region of the segmentation gene fushi tarazu (ftz). Gain-of-function mutations in Scr result in the presence of ectopic sex comb teeth on the first tarsal segment of mesothoracic and metathoracic legs of adult males. Heterozygous combinations of gain-of-function alleles with a wild-type Scr gene exhibit no evidence of ectopic protein localization in the second and third thoracic segments of embryos. However, mesothoracic and metathoracic leg imaginal discs can be shown to accumulate ectopically expressed Scr protein, implying a differential regulation of the Scr gene during these two periods of development. Additionally, we have found that the spatial pattern of Scr gene expression in imaginal tissues involved in the development of the adult thorax is governed in part by synapsis of homologous chromosomes in this region of the ANT-C. However, those imaginal discs that arise anteriorly to the prothorax do not appear to be sensitive to this form of gene regulation. Finally, we have demonstrated that the extent of Scr expression is influenced by mutations at the Polycomb (Pc) locus but not by mutant alleles of the zeste (z) gene. Taken together, our data suggests that Scr gene expression is differentially regulated both temporally and spatially in a manner that is sensitive to the structure of the locus.


Land ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 679
Author(s):  
Avi Bar-Massada

The Wildland Urban Interface (WUI) is where human settlements border or intermingle with undeveloped land, often with multiple detrimental consequences. Therefore, mapping the WUI is required in order to identify areas-at-risk. There are two main WUI mapping methods, the point-based approach and the zonal approach. Both differ in data requirements and may produce considerably different maps, yet they were never compared before. My objective was to systematically compare the point-based and the zonal-based WUI maps of California, and to test the efficacy of a new database of building locations in the context of WUI mapping. I assessed the spatial accuracy of the building database, and then compared the spatial patterns of WUI maps by estimating the effect of multiple ancillary variables on the amount of agreement between maps. I found that the building database is highly accurate and is suitable for WUI mapping. The point-based approach estimated a consistently larger WUI area across California compared to the zonal approach. The spatial correspondence between maps was low-to-moderate, and was significantly affected by building numbers and by their spatial arrangement. The discrepancy between WUI maps suggests that they are not directly comparable within and across landscapes, and that each WUI map should serve a distinct practical purpose.


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