The Effect of Low Dose Rate on Recovery of Hemopoietic and Stromal Progenitor Cells in γ-Irradiated Mouse Bone Marrow

1988 ◽  
Vol 115 (3) ◽  
pp. 481 ◽  
Author(s):  
R. Gallini ◽  
J. H. Hendry ◽  
G. Molineux ◽  
N. G. Testa
Keyword(s):  
Bone Marrow ◽  
Dose Rate ◽  
Progenitor Cells ◽  
Low Dose ◽  
Mouse Bone Marrow ◽  
Low Dose Rate ◽  
Stromal Progenitor Cells

scholarly journals Syngeneic and allogeneic bone marrow engraftment after total body irradiation: dependence on dose, dose rate, and fractionation

Blood ◽  
1991 ◽  
Vol 77 (3) ◽  
pp. 661-669 ◽  
Author(s):  
JD Down ◽  
NJ Tarbell ◽  
HD Thames ◽  
PM Mauch
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Radiation Dose ◽  
Dose Rate ◽  
Dose Response ◽  
Total Body Irradiation ◽  
Low Dose ◽  
Allogeneic Bone ◽  
Low Dose Rate ◽  
Total Body

Abstract Murine bone marrow chimera models were used to assess the efficacy of host total body irradiation (TBI) given at different doses, dose rates, and fractionation schemes in providing for engraftment of syngeneic and allogeneic bone marrow. B6-Hbbd congenic and LP mice, respectively, were used as donors (10(7) bone marrow cells) for syngeneic and allogenic (H-2 compatible) transplantation in standard B6 recipients. Stable marrow chimerism was determined from host and donor stem cell- derived hemoglobin phenotypes (Hbbs and Hbbd) on gel electrophoresis at 3 months posttransplant. Partial engraftment of syngeneic marrow was seen at single doses as low as 2 Gy, with the donor component increasing steadily with increasing TBI dose to a level of 100% at 7 Gy. Immunologic resistance of the host appeared to prevent allogeneic engraftment until 5.5 Gy. A very steep radiation dose response was then observed so that the level of chimerism with 6 Gy and above became comparable with syngeneic engraftment. Low dose rate (5 cGy minute-1) and fractionated TBI required higher total doses for equivalent engraftment (radiation dose-sparing) in both syngeneic and allogenic bone marrow transplantation. This displacement in the dose-response curve on fractionation was seen with interfraction intervals of 3 and 6 hours. A further dose-sparing effect was observed on extending the interval to 18 and 24 hours, but only for allogeneic transplantation, and may therefore be related to recovery of immune-mediated graft resistance. The involvement of multiple target cell populations in determining allogenic engraftment rendered the application of the linear-quadratic model for radiation cell survival problematic in this case. The recovery in dose when low dose rate and 6-hour interfraction intervals were applied in either syngeneic or allogeneic BMT is consistent with appreciable sub-lethal damage repair in the primitive self-renewing stem cell population of the host marrow. These results contrast with the poor repair capacity of the 11-day spleen colony- forming units (CFUs) population after fractionated irradiation and support the notion that ablation of early stem cells in the pre-CFUs compartment is essential for long-term marrow engraftment.

scholarly journals Syngeneic and allogeneic bone marrow engraftment after total body irradiation: dependence on dose, dose rate, and fractionation

Blood ◽  
1991 ◽  
Vol 77 (3) ◽  
pp. 661-669 ◽  
Author(s):  
JD Down ◽  
NJ Tarbell ◽  
HD Thames ◽  
PM Mauch
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Radiation Dose ◽  
Dose Rate ◽  
Dose Response ◽  
Total Body Irradiation ◽  
Low Dose ◽  
Allogeneic Bone ◽  
Low Dose Rate ◽  
Total Body

Murine bone marrow chimera models were used to assess the efficacy of host total body irradiation (TBI) given at different doses, dose rates, and fractionation schemes in providing for engraftment of syngeneic and allogeneic bone marrow. B6-Hbbd congenic and LP mice, respectively, were used as donors (10(7) bone marrow cells) for syngeneic and allogenic (H-2 compatible) transplantation in standard B6 recipients. Stable marrow chimerism was determined from host and donor stem cell- derived hemoglobin phenotypes (Hbbs and Hbbd) on gel electrophoresis at 3 months posttransplant. Partial engraftment of syngeneic marrow was seen at single doses as low as 2 Gy, with the donor component increasing steadily with increasing TBI dose to a level of 100% at 7 Gy. Immunologic resistance of the host appeared to prevent allogeneic engraftment until 5.5 Gy. A very steep radiation dose response was then observed so that the level of chimerism with 6 Gy and above became comparable with syngeneic engraftment. Low dose rate (5 cGy minute-1) and fractionated TBI required higher total doses for equivalent engraftment (radiation dose-sparing) in both syngeneic and allogenic bone marrow transplantation. This displacement in the dose-response curve on fractionation was seen with interfraction intervals of 3 and 6 hours. A further dose-sparing effect was observed on extending the interval to 18 and 24 hours, but only for allogeneic transplantation, and may therefore be related to recovery of immune-mediated graft resistance. The involvement of multiple target cell populations in determining allogenic engraftment rendered the application of the linear-quadratic model for radiation cell survival problematic in this case. The recovery in dose when low dose rate and 6-hour interfraction intervals were applied in either syngeneic or allogeneic BMT is consistent with appreciable sub-lethal damage repair in the primitive self-renewing stem cell population of the host marrow. These results contrast with the poor repair capacity of the 11-day spleen colony- forming units (CFUs) population after fractionated irradiation and support the notion that ablation of early stem cells in the pre-CFUs compartment is essential for long-term marrow engraftment.

Recombinant murine GM-CSF increases resistance of some factor dependent hematopoietic progenitor cells to low-dose-rate gamma irradiation

1989 ◽  
Vol 17 (2) ◽  
pp. 323-335 ◽  
Author(s):  
T.J. Fitzgerald ◽  
S. Henault ◽  
M.A. Santucci ◽  
P. Anklesaria ◽  
S. Zak ◽  
...  
Keyword(s):  
Gamma Irradiation ◽  
Dose Rate ◽  
Progenitor Cells ◽  
Low Dose ◽  
Hematopoietic Progenitor Cells ◽  
Hematopoietic Progenitor ◽  
Gm Csf ◽  
Low Dose Rate

Adaptive response of bone marrow stem cells induced by low-dose rate irradiation in C57BL/6 mice

2005 ◽  
Vol 1276 ◽  
pp. 264-265 ◽  
Author(s):  
Kazuniori Shiraishi ◽  
Akira Tachibana ◽  
Morio Yonezawa ◽  
Seiji Kodama
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Dose Rate ◽  
Adaptive Response ◽  
Low Dose ◽  
Bone Marrow Stem Cells ◽  
Low Dose Rate

scholarly journals Comparative mathematical analysis of the colony-forming ability of bone marrow of mice irradiated in lethal dose with high and low dose rate

2018 ◽  
Vol 19 (3) ◽  
pp. 265-269
Author(s):  
R.V. Boiko ◽  
D.I. Bіlko ◽  
I.Z. Russu ◽  
N.M. Bіlko
Keyword(s):  
Bone Marrow ◽  
Dose Rate ◽  
Mathematical Analysis ◽  
Low Dose ◽  
Lethal Dose ◽  
Low Dose Rate

Interstitial pneumonitis following bone marrow transplantation using fractionated low dose rate total body irradiation (TBI)

1992 ◽  
Vol 24 ◽  
pp. 276-277
Author(s):  
Michael G. Chen ◽  
Louis Letendre ◽  
H.Clark Hoagland ◽  
Michelle M. Ryks ◽  
Mayo Bone Marrow Transplant Team
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Dose Rate ◽  
Total Body Irradiation ◽  
Interstitial Pneumonitis ◽  
Marrow Transplantation ◽  
Low Dose ◽  
Low Dose Rate ◽  
Total Body

Low-dose rate brachytherapy for patients with low or intermediate-risk prostate cancer. Analysis of relapse-free survival

2019 ◽  
Vol 11 (2) ◽  
pp. 44-49
Author(s):  
V.A. Solodky ◽  
◽  
A.Yu. Pavlov ◽  
A.D. Tsybulskiy ◽  
A.S. Pchelintsev ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Dose Rate ◽  
Low Dose ◽  
Intermediate Risk ◽  
Relapse Free Survival ◽  
Free Survival ◽  
Low Dose Rate ◽  
Risk Prostate Cancer ◽  
Low Dose Rate Brachytherapy
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