Isolation of Auxotrophic Mutants of Allomyces macrogynus

Mycologia ◽  
1981 ◽  
Vol 73 (3) ◽  
pp. 493 ◽  
Author(s):  
Lauritz W. Olson ◽  
Tom A. B. Nielsen
Keyword(s):  
Auxotrophic Mutants ◽  
Allomyces Macrogynus

Enrichment of Auxotrophic Mutants of Allomyces Macrogynus by a Sticky Technique

Mycologia ◽  
1982 ◽  
Vol 74 (4) ◽  
pp. 668-670 ◽  
Author(s):  
Tom A. B. Nielsen
Keyword(s):  
Auxotrophic Mutants ◽  
Allomyces Macrogynus

Enrichment of Auxotrophic Mutants of Allomyces macrogynus by a Sticky Technique

Mycologia ◽  
1982 ◽  
Vol 74 (4) ◽  
pp. 668
Author(s):  
Tom A. B. Nielsen
Keyword(s):  
Auxotrophic Mutants ◽  
Allomyces Macrogynus

scholarly journals Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae

1995 ◽  
Vol 52 (3) ◽  
pp. 548-554 ◽  
Author(s):  
V. Kava - Cordeiro ◽  
E.A. Luna - Alves - Lima ◽  
J.L. Azevedo
Keyword(s):  
Gamma Radiation ◽  
Ultraviolet Light ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Nitrous Acid ◽  
Wild Strain ◽  
Wild Type ◽  
Survival Curves ◽  
Auxotrophic Mutants ◽  
Morphological Mutants

A wild strain of Metarhizium anisopliae, an entomopathogenic fungus, was submitted to three mutagenic agents: gamma radiation, ultraviolet light and nitrous acid. Survival curves were obtained and mutants were selected using different mutagenic doses which gave 1 to 5% survival. Morphological and auxotrophic mutants were isolated. Morphological mutants were grouped in a class with yellow conidia and other with pale vinaceous conidia as opposed to the green wild type conidia. Auxotrophic mutants had requirements for vitamin and aminoacid biosynthesis. More than 58% of the total auxotrophk mutants required proline/aipnine. Gamma radiation showed to be the most efficient mutagenic agent giving 0.2% of auxotrophk mutants followed by ultraviolet light (0.12%) and nitrous acid (0.06%).The conidial colour and auxotrophk mutants isolated until now from M. anisopliae were reviewed.

The Effects of Sterol Biosynthesis Inhibitors on Cleavage in Allomyces macrogynus

Mycologia ◽  
1988 ◽  
Vol 80 (5) ◽  
pp. 716 ◽  
Author(s):  
Melvin S. Fuller ◽  
Robert W. Roberson
Keyword(s):  
Sterol Biosynthesis ◽  
Allomyces Macrogynus ◽  
Biosynthesis Inhibitors

The cytology of the gametes and fertilization of Allomyces macrogynus

1976 ◽  
Vol 109 (1-2) ◽  
pp. 21-30 ◽  
Author(s):  
Jeffrey Pommerville ◽  
Melvin S. Fuller
Keyword(s):  
Allomyces Macrogynus

scholarly journals Protection Elicited by Two Glutamine Auxotrophs of Mycobacterium tuberculosis and In Vivo Growth Phenotypes of the Four Unique Glutamine Synthetase Mutants in a Murine Model

2006 ◽  
Vol 74 (11) ◽  
pp. 6491-6495 ◽  
Author(s):  
Sunhee Lee ◽  
Bo-Young Jeon ◽  
Svetoslav Bardarov ◽  
Mei Chen ◽  
Sheldon L. Morris ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Glutamine Synthetase ◽  
Mycobacterium Bovis ◽  
Murine Model ◽  
Triple Mutant ◽  
Auxotrophic Mutants ◽  
Bcg Vaccination ◽  
In Vivo Growth ◽  
Subcutaneous Immunization

ABSTRACT We generated four individual glutamine synthetase (GS) mutants (ΔglnA1, ΔglnA2, ΔglnA3, and ΔglnA4) and one triple mutant (ΔglnA1EA2) of Mycobacterium tuberculosis to investigate the roles of GS enzymes. Subcutaneous immunization with the ΔglnA1EA2 and ΔglnA1 glutamine auxotrophic mutants conferred protection on C57BL/6 mice against an aerosol challenge with virulent M. tuberculosis, which was comparable to that provided by Mycobacterium bovis BCG vaccination.

scholarly journals Induction of Auxotrophic Mutants from Candida Species and their Application to L-Threonine Fermentation

1971 ◽  
Vol 35 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Yoji TSUKADA ◽  
Tsunetake SUGIMORI
Keyword(s):  
Candida Species ◽  
Auxotrophic Mutants

Production of carminomycin by auxotrophic mutants of Actinomadura carminata

1975 ◽  
Vol 15 (5) ◽  
pp. 383-385 ◽  
Author(s):  
O. A. Lapchinskaya ◽  
T. A. Saburova ◽  
V. I. Ponomarenko
Keyword(s):  
Auxotrophic Mutants
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