Characterization of Aphid Pathogenic Species in the Genus Pandora by PCR Techniques and Digital Image Analysis

Mycologia ◽  
2001 ◽  
Vol 93 (5) ◽  
pp. 864 ◽  
Author(s):  
Charlotte Nielsen ◽  
Christian Sommer ◽  
Jorgen Eilenberg ◽  
Karen S. Hansen ◽  
Richard A. Humber
Mycologia ◽  
2001 ◽  
Vol 93 (5) ◽  
pp. 864-874 ◽  
Author(s):  
Charlotte Nielsen ◽  
Christian Sommer ◽  
Jørgen Eilenberg ◽  
Karen S. Hansen ◽  
Richard A. Humber

Author(s):  
Rosinei Batista ◽  
Gilbert Silva ◽  
Antnio Henriques Araujo ◽  
Nelson Tavares ◽  
Jos Wilson De Jesus Silva ◽  
...  

1992 ◽  
Vol 31 (8) ◽  
pp. 1083
Author(s):  
Rajeeb Hazra ◽  
Charles L. Viles ◽  
Stephen K. Park ◽  
Stephen E. Reichenbach ◽  
Michael E. Sieracki

2019 ◽  
Vol 8 (2) ◽  
pp. 147-161
Author(s):  
Anisa Lailatusy Syarifah ◽  
◽  
Rurini Retnowati ◽  
Hermin Sulistyarti

Curcuma longa, C. xanthorrhiza, C. heyneana, and Zingiber cassumunar contain high curcuminoid and have relatively similar yellow color. Therefore, they are potentially adulterated and difficult to differentiate in the form of powder. Hence, it is necessary to characterize the fingerprints compound profile by a simple and rapid method. This research aims to determine fingerprint compound profile of curcuminoid using Thin Layer Chromatography (TLC) and digital image analysis. The result of the research identified that the fingerprint compound profile of curcuminoid on the four rhizomes was obtained by TLC method using silica gel 60 GF254 as the stationary phase, chloroform: dichloromethane: methanol (13:6:1) as the mobile phase, and observation under UV 254 nm light and citroborate reagent. Thereafter, the digital image analysis was carried out using Image J software according to the gray value and % of RGB (red-green-blue) value. Based on gray value and % of RGB, both Curcuma and Zingiber genera were differentiated through curcumin compound (Rf 0.63), demethoxycurcumin (Rf 0.34), bisdemethoxycurcumin (Rf 0.21). The profile of fingerprint compound on Curcuma longa, C. xanthorrhiza, C. heyneana, and Zingiber cassumunar was differentiated through Rf 0.26; Rf 0.17; and Rf 0.10.


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