scholarly journals Detection and Identification of Infectious Hematopoietic Necrosis Virus (IHNV) by Reverse Transcription (RT)-polymerase Chain Reaction (PCR)

1997 ◽  
Vol 63 (4) ◽  
pp. 592-595 ◽  
Author(s):  
Toko Yoshinaka ◽  
Mamoru Yoshimizu ◽  
Tomoo Sawabe ◽  
Yoshio Ezura
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Infectious Hematopoietic Necrosis Virus ◽  
Necrosis Virus ◽  
Chain Reaction ◽  
Detection And Identification ◽  
Infectious Hematopoietic Necrosis ◽  
Polymerase Chain

scholarly journals A One-Step Reverse Transcription-Polymerase Chain Reaction System for the Simultaneous Detection and Identification of Multiple Tospovirus Infections

2005 ◽  
Vol 95 (2) ◽  
pp. 166-171 ◽  
Author(s):  
Hiroyuki Uga ◽  
Shinya Tsuda
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Simultaneous Detection ◽  
Yellow Spot ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Spot Virus ◽  
Detection And Identification ◽  
One Step ◽  
Polymerase Chain

A one-step reverse transcription-polymerase chain reaction (RT-PCR) method has been developed for the simultaneous detection and identification of multiple tospoviruses that infect plants. The RT-PCR system is composed of six primers in a single tube: a universal degenerate primer and five virus species-specific primers. Amplifications resulted in an 848-bp PCR product for Watermelon silver mottle virus, 709-bp for Tomato spotted wilt virus, 589-bp for Impatiens necrotic spot virus, 511-bp for Melon yellow spot virus, and a 459-bp amplicon for Iris yellow spot virus. This system enables the simultaneous detection of at least three types of tospovirus infections, in addition to their species identities, from five possible tospoviruses studied, on the basis of their S RNA combinations. This multiplex RT-PCR system was applied to the detection of tospovirus in ornamental crops cultivated in fields and shows potential for epidemiological studies.

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