In-air PIXE was used to analyze trace elements in otoliths from several species of teleost fish in order to examine the correlation between the trace elements concentrations and environmental conditions. Mn, Fe, Zn and Sr were detected accurately in the order of ppm by using the in-air PIXE. It seemed that concentrations of Sr and Zn in red sea bream otoliths increased in proportion to higher seawater temperature. In addition, there were significant differences in trace element composition between that of reared red sea bream and rockfish and that of wild ones. Preliminary results indicate that the PIXE is a powerful technique to investigate fish otoliths.
Requirement of red sea bream for dietary trace elements.