Simultaneous Determination of Antigen Specific IgE Antibodies by MAST(Multiple Antigen Simultaneous Test) System in the Patients with Atopic Dermatitis.

1991 ◽  
Vol 53 (4) ◽  
pp. 762-766
Author(s):  
Osamu KORO ◽  
Eishin MORITA ◽  
Kohji NAKAMURA ◽  
Satoru YAMADA ◽  
Shoso YAMAMOTO
Author(s):  
T. S. Okuneva ◽  
M. V. Kushnareva ◽  
A. N. Pampura ◽  
E. E. Varlamov ◽  
N. G. Konyukova

We studied the frequency of occurrence and concentration of allergen-specific IgE antibodies (asIgE) to enterotoxins A, B, C and TSST-1 of Staphylococcus aureus and Malassezia spp. in the serum of children with atopic dermatitis in the acute period of the disease. The determination of asIgE levels was performed in 84 children aged from 5 months to 17 years using the immunochemiluminescent method on the UniCAP 100 analyzer (Phadia, Sweden). The level of asIgE was evaluated on a scale of 6 classes.The sensitization to S. aureus enterotoxins was detected in 29 children with аsIgE concentration from 0.35 to 25.8 kU/L (I–IV classes). In 16 children were observed 8 polysensitization options to S. aureus allergens. Combinations of asIgE to A + B and A + B + C enterotoxins were most common – in every fourth and fifth child,respectively. Sensitization to Malassezia spp. was observed in 41 children with concentration from 0.38 to 98.9 KU/L (I–V classes). Polysensitization to both S. aureus and Malassezia spp. allergens was observed in one of two children. A higher degree of sensitisation to Malassezia spp allergens has been established compared to S. aureus.Conclusion. To improve the specific diagnostics of atopic dermatitis, it is advisable to examine the serum for a complete panel of recombinant S. aureus enterotoxins in combination with the determination of asIgE to Malassezia spp. 


Thorax ◽  
2015 ◽  
Vol 70 (Suppl 3) ◽  
pp. A102.2-A103
Author(s):  
J Canizales ◽  
J Welch ◽  
B Fitzgerald ◽  
Z Lightfoot ◽  
W Banya ◽  
...  

1994 ◽  
Vol 36 (2) ◽  
pp. 146-149 ◽  
Author(s):  
ATSUO URISU ◽  
YASUTO KONDO ◽  
EIKO WADA ◽  
FUMIYA HORIBA ◽  
MITSUTOSI TSURUTA ◽  
...  

2013 ◽  
Vol 63 (4) ◽  
pp. 437-451 ◽  
Author(s):  
Natalija Milcic-Matic ◽  
Jana Ognjenovic ◽  
Lidija Burazer ◽  
G. Blagojevic ◽  
N. Popovic ◽  
...  

1996 ◽  
Vol 21 (5) ◽  
pp. 347-350 ◽  
Author(s):  
K. JUNG ◽  
G. SCHLENVOIGT ◽  
K. LADWIG ◽  
D. HERRMANN ◽  
C. MOTHS ◽  
...  

1991 ◽  
Vol 94 (1-4) ◽  
pp. 376-376 ◽  
Author(s):  
M. Blanca ◽  
J.M. Vega ◽  
E. Perez ◽  
M.J. Carmona ◽  
A. Miranda ◽  
...  

2004 ◽  
Vol 46 (3) ◽  
pp. 337-341 ◽  
Author(s):  
Fumihito Ide ◽  
Tomoyo Matsubara ◽  
Miho Kaneko ◽  
Takashi Ichiyama ◽  
Tokuko Mukouyama ◽  
...  

2011 ◽  
Vol 8 (4) ◽  
pp. 55-61
Author(s):  
N G Konyukova ◽  
A N Pampura ◽  
E S Fedenko ◽  
A I Khavkin ◽  
T S Okuneva ◽  
...  

Background. To establish the clinical and immunological features of the manifestations of allergic diseases in children with IgE-mediated sensitization to potato. Methods. allergy tests (skin prick tests and / or the determination of specific IgE in serum by ImmunoCap, Phadia was performed in 171 children with allergic diseases, who had sensitization to food plant proteins. Results. sensitization to the potato found in 134 children. significant discrepancies (36%) in the results of in vitro and in vivo tests to the potatoes were shown. sensitization to the potatoes is more common in patients with atopic dermatitis (ad), especially in severe disease. among children with the level of specific IgE antibodies to potato ≥2 kUa / l there were more common patients with severe ad. the correlation between the level of specific IgE antibodies to the potatoes and some of food plant proteins was found. the relationship between the concentration of specific IgE to the potatoes and Bet v 2 was not detected. Conclusion. the presence and concentration of specific IgE to the potatoes to a certain extent associated with clinical manifestations of ad. the data obtained substantiate the determination of specific IgE antibodies to potato in children with ad, especially in severe cases.


RADIOISOTOPES ◽  
1990 ◽  
Vol 39 (10) ◽  
pp. 449-452
Author(s):  
Akio YOSHIMOTO ◽  
Mitsuyoshi YAZAKI ◽  
Takao SUGAYA

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