CLINICAL SIGNIFICANCE OF DETERMINATION OF IGE ANTIBODIES TO STAPHYLOCOCCUS AUREUS AND MALASSEZIA SPECIES IN SERUM OF CHILDREN WITH ATOPIC DERMATITIS

We studied the frequency of occurrence and concentration of allergen-specific IgE antibodies (asIgE) to enterotoxins A, B, C and TSST-1 of Staphylococcus aureus and Malassezia spp. in the serum of children with atopic dermatitis in the acute period of the disease. The determination of asIgE levels was performed in 84 children aged from 5 months to 17 years using the immunochemiluminescent method on the UniCAP 100 analyzer (Phadia, Sweden). The level of asIgE was evaluated on a scale of 6 classes.The sensitization to S. aureus enterotoxins was detected in 29 children with аsIgE concentration from 0.35 to 25.8 kU/L (I–IV classes). In 16 children were observed 8 polysensitization options to S. aureus allergens. Combinations of asIgE to A + B and A + B + C enterotoxins were most common – in every fourth and fifth child,respectively. Sensitization to Malassezia spp. was observed in 41 children with concentration from 0.38 to 98.9 KU/L (I–V classes). Polysensitization to both S. aureus and Malassezia spp. allergens was observed in one of two children. A higher degree of sensitisation to Malassezia spp allergens has been established compared to S. aureus.Conclusion. To improve the specific diagnostics of atopic dermatitis, it is advisable to examine the serum for a complete panel of recombinant S. aureus enterotoxins in combination with the determination of asIgE to Malassezia spp.

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Anti-Malassezia-Specific IgE Antibodies Production in Japanese Patients with Head and Neck Atopic Dermatitis: Relationship between the Level of Specific IgE Antibody and the Colonization Frequency of Cutaneous Malassezia Species and Clinical Severity

Journal of Allergy ◽

10.1155/2011/645670 ◽

2011 ◽

Vol 2011 ◽

pp. 1-5 ◽

Cited By ~ 11

Author(s):

Enshi Zhang ◽

Takafumi Tanaka ◽

Mami Tajima ◽

Ryoji Tsuboi ◽

Hiroshi Kato ◽

...

Keyword(s):

Atopic Dermatitis ◽

Head And Neck ◽

Japanese Patients ◽

Specific Ige ◽

Clinical Severity ◽

Ige Antibodies ◽

Malassezia Species ◽

Colonization Frequency ◽

Specific Ige Antibodies ◽

Species Specific

Atopic dermatitis of the head and neck (HNAD) is recognized as a separate condition. Malassezia, the predominant skin microbiota fungus, is considered to exacerbate atopic dermatitis (AD), especially HNAD. In the present study, we investigated the relationships between the levels of specific IgE antibodies, colonization frequency of eight predominant Malassezia species, and clinical severity in 61 patients with HNAD (26 mild, 24 moderate, and 11 severe cases). As clinical severity increased, the levels of specific IgE antibodies against eight Malassezia species also increased. Species diversity of the Malassezia microbiota in scale samples from patients was analyzed by nested PCR using species-specific primers. The clinical severity of HNAD was correlated with the total level of specific IgE antibodies against Malassezia species and the number of Malassezia species detected.

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