111-OR: Pancreatic Elastase Regulates Human Beta-Cell Proliferation via the Focal Adhesion and PAR2 Signaling Pathways

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 111-OR
Author(s):  
GIORGIO BASILE ◽  
AMEDEO VETERE ◽  
KA-CHEUK LIU ◽  
JIANG HU ◽  
OLOV ANDERSSON ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Beta Cell ◽  
Signaling Pathways ◽  
Focal Adhesion ◽  
Beta Cell Proliferation ◽  
Pancreatic Elastase ◽  
Human Beta Cell

Identification of Pancreatic Elastase Inhibitors That Can Stimulate Beta-Cell Proliferation

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 49-OR
Author(s):  
GIORGIO BASILE ◽  
AMEDEO VETERE ◽  
JIANG HU ◽  
BRIDGET WAGNER ◽  
ROHIT KULKARNI
Keyword(s):  
Cell Proliferation ◽  
Beta Cell ◽  
Beta Cell Proliferation ◽  
Pancreatic Elastase

Glucose Increases Human Beta Cell Proliferation In Vitro and In Vivo.

2010 ◽  
pp. P3-482-P3-482
Author(s):  
HE Levitt ◽  
TJ Cyphert ◽  
JL Pascoe ◽  
DA Hollern ◽  
N Abraham ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Beta Cell ◽  
Beta Cell Proliferation ◽  
Human Beta Cell ◽  
Proliferation In Vitro

scholarly journals Functional Genomic HTS to Study Primary Human Beta-Cell Proliferation

2013 ◽  
Vol 37 ◽  
pp. S58
Author(s):  
Karine Robitaille ◽  
Accalia Fu ◽  
Stephen Baird ◽  
Joanne E. Mcbane ◽  
Qiujiang Du ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Beta Cell ◽  
Beta Cell Proliferation ◽  
Functional Genomic ◽  
Human Beta Cell

scholarly journals Osteoprotegerin and Denosumab Stimulate Human Beta Cell Proliferation through Inhibition of the Receptor Activator of NF-κB Ligand Pathway

2015 ◽  
Vol 22 (1) ◽  
pp. 77-85 ◽  
Author(s):  
Nagesha Guthalu Kondegowda ◽  
Rafael Fenutria ◽  
Ilana R. Pollack ◽  
Michael Orthofer ◽  
Adolfo Garcia-Ocaña ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Beta Cell ◽  
Beta Cell Proliferation ◽  
Receptor Activator ◽  
Human Beta Cell

scholarly journals The Harmine and Exendin-4 Combination Markedly Expands Human Beta Cell Mass In Vivo: Quantification and Visualization By iDISCO+ 3D Imaging

2020 ◽  
Author(s):  
Carolina Rosselot ◽  
Alexandra Alvarsson ◽  
Peng Wang ◽  
Yansui Li ◽  
Kunal Kumar ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Beta Cell ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Cell Mass ◽  
Beta Cell Mass ◽  
Beta Cell Proliferation ◽  
Light Sheet Microscopy ◽  
Human Beta Cell

AbstractSince all diabetes results from reductions in numbers of functional pancreatic beta cells, beta cell regenerative drugs are required for optimal and scalable future diabetes treatment. While many diabetes drugs are in clinical use, none increases human beta cell numbers. We have shown that a combination of the DYRK1A inhibitor, harmine, with the GLP1 receptor agonist, exendin-4, markedly increases human beta cell proliferation in vitro. However, technological limitations have prevented assessment of human beta cell mass in vivo. Here, we describe a novel method that combines iDISCO+ tissue clearing, insulin immunolabeling, light sheet microscopy, and volumetric quantification of human beta cells transplanted into immunodeficient mice. We demonstrate a striking seven-fold in vivo increase in human beta cell mass in response to three months of combined harmine-exendin-4 combination infusion, accompanied by lower blood glucose levels, increased plasma human insulin concentrations and enhanced beta cell proliferation. These studies unequivocally demonstrate for the first time that pharmacologic human beta cell expansion is a realistic and achievable path to diabetes therapy, and provide a rigorous, entirely novel and reproducible tool for quantifying human beta cell mass in vivo.

scholarly journals High-throughput Functional Genomics Identifies Regulators of Primary Human Beta Cell Proliferation

2016 ◽  
Vol 291 (9) ◽  
pp. 4614-4625 ◽  
Author(s):  
Karine Robitaille ◽  
Jillian L. Rourke ◽  
Joanne E. McBane ◽  
Accalia Fu ◽  
Stephen Baird ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Beta Cell ◽  
Functional Genomics ◽  
High Throughput ◽  
Beta Cell Proliferation ◽  
Human Beta Cell

Hormonal control of [beta]-cell proliferation in fetal ovine pancreatic isletsin vitro

2015 ◽  
Author(s):  
Shelley E Harris ◽  
Amy Kelly ◽  
Melissa A Davis ◽  
Miranda Anderson ◽  
Alison J Forhead ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Beta Cell ◽  
Hormonal Control ◽  
Beta Cell Proliferation

scholarly journals Hepatocyte Growth Factor Overexpression in the Islet of Transgenic Mice Increases Beta Cell Proliferation, Enhances Islet Mass, and Induces Mild Hypoglycemia

2000 ◽  
Vol 275 (2) ◽  
pp. 1226-1232 ◽  
Author(s):  
Adolfo Garcia-Ocaña ◽  
Karen K. Takane ◽  
Mushtaq A. Syed ◽  
William M. Philbrick ◽  
Rupangi C. Vasavada ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Growth Factor ◽  
Transgenic Mice ◽  
Hepatocyte Growth Factor ◽  
Beta Cell ◽  
Beta Cell Proliferation ◽  
Islet Mass ◽  
Hepatocyte Growth
Sign in / Sign up

Export Citation Format

Share Document