The role of BRAF V600E mutation in post-surgical 131I therapy in papillary thyroid carcinoma. A study based on SPECT-CT uptake analysis

Background: Thyroid carcinoma (TC) is the most common endocrine system malignancy, and papillary thyroid carcinoma (PTC) accounts for >80% of all TC cases. Nevertheless, PTC pathogenesis is still not fully understood. The aim of the study was to elucidate the role of the FRMD5 protein in the regulation of biological pathways associated with the development of PTC. We imply that the presence of certain genetic aberrations (e.g., BRAF V600E mutation) is associated with the activity of FRMD5. Methods: The studies were conducted on TPC1 and BCPAP (BRAF V600E) model PTC-derived cells. Transfection with siRNA was used to deplete the expression of FRMD5. The mRNA expression and protein yield were evaluated using RT-qPCR and Western blot techniques. Proliferation, migration, invasiveness, adhesion, spheroid formation, and survival tests were performed. RNA sequencing and phospho-kinase proteome profiling were used to assess signaling pathways associated with the FRMD5 expressional status. Results: The obtained data indicate that the expression of FRMD5 is significantly enhanced in BRAF V600E tumor specimens and cells. It was observed that a drop in intracellular yield of FRMD5 results in significant alternations in the migration, invasiveness, adhesion, and spheroid formation potential of PTC-derived cells. Importantly, significant divergences in the effect of FRMD5 depletion in both BRAF-wt and BRAF-mutated PTC cells were observed. It was also found that knockdown of FRMD5 significantly alters the expression of multidrug resistant genes. Conclusions: This is the first report highlighting the importance of the FRMD5 protein in the biology of PTCs. The results suggest that the FRMD5 protein can play an important role in controlling the metastatic potential and multidrug resistance of thyroid tumor cells.

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Detection of BRAF V600E mutation in fine-needle aspiration fluid of papillary thyroid carcinoma by droplet digital PCR

Clinica Chimica Acta ◽

10.1016/j.cca.2019.01.017 ◽

2019 ◽

Vol 491 ◽

pp. 91-96 ◽

Cited By ~ 6

Author(s):

Xiao Xu ◽

Xiaowei Ma ◽

Xinju Zhang ◽

Guojun Cao ◽

Yigui Tang ◽

...

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Fine Needle Aspiration ◽

Digital Pcr ◽

Needle Aspiration ◽

Droplet Digital Pcr ◽

Braf V600e Mutation ◽

Braf V600e ◽

Papillary Thyroid ◽

Fine Needle

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Imbalance in DNA repair machinery is associated with BRAF V600E mutation and tumor aggressiveness in papillary thyroid carcinoma

Molecular and Cellular Endocrinology ◽

10.1016/j.mce.2017.12.004 ◽

2018 ◽

Vol 472 ◽

pp. 140-148 ◽

Cited By ~ 8

Author(s):

Bruna S. Lutz ◽

Natalia M. Leguisamo ◽

Nicole K. Cabral ◽

Helena C. Gloria ◽

Keli C. Reiter ◽

...

Keyword(s):

Dna Repair ◽

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Braf V600e Mutation ◽

Braf V600e ◽

Papillary Thyroid ◽

Tumor Aggressiveness

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Clinical utility of immunohistochemistry for the detection of the BRAF v600e mutation in papillary thyroid carcinoma

Surgery ◽

10.1016/j.surg.2013.06.020 ◽

2013 ◽

Vol 154 (6) ◽

pp. 1199-1205 ◽

Cited By ~ 34

Author(s):

Jonathan Zagzag ◽

Aron Pollack ◽

Linda Dultz ◽

Shumon Dhar ◽

Jennifer B. Ogilvie ◽

...

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Clinical Utility ◽

Braf V600e Mutation ◽

Braf V600e ◽

Papillary Thyroid

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Correlation between BRAF V600E mutation and clinicopathological features in pediatric papillary thyroid carcinoma

Science China Life Sciences ◽

10.1007/s11427-017-9083-8 ◽

2017 ◽

Vol 60 (7) ◽

pp. 729-738 ◽

Cited By ~ 13

Author(s):

Jiangqiao Geng ◽

Huanmin Wang ◽

Yuanhu Liu ◽

Jun Tai ◽

Yaqiong Jin ◽

...

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Braf V600e Mutation ◽

Clinicopathological Features ◽

Braf V600e ◽

Papillary Thyroid

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BRAF V600E Immunoexpression in Papillary Thyroid Carcinoma and Its Association with Prognostic Factors and Histopathologic Variant

Medicinus ◽

10.19166/med.v8i1.3120 ◽

2021 ◽

Vol 8 (1) ◽

pp. 12

Author(s):

Erna Kristiani ◽

Endang SR Hardjolukito ◽

Agnes S Harahap ◽

Benyamin Makes

Keyword(s):

Prognostic Factors ◽

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Clinicopathological Characteristics ◽

Braf V600e Mutation ◽

Braf V600e ◽

Papillary Thyroid ◽

Tall Cell Variant ◽

Tall Cell ◽

Cell Variant

Aim: to provide additional information regarding the clinicopathological characteristics of Papillary Thyroid Carcinoma (PTC). Methods: Fifty patient with PTC were reviewed to determine prognostic factors such as age, gender, size of tumor and histologic variant. BRAF V600E mutation was detected by immunohistochemical staining and assessed with H score. Result: BRAF V600E mutations were detected in 17 (34%) cases. There were seven cases with extrathyroidal extension (ETE) p 0,04, 11 cases with lymph node metastasis (LNM) p < 0,001, and 8 cases with tall cell variant p 0,047.The cases with positive BRAF V600E mutation had mean age of 44.71 years, and the size of the tumor between 0.1-4cm. Six cases of them are male and 11 female.Conclusion: There were significant relationships between BRAF V600E mutation with ETE, LNM, and tall cell variant. There was no significant relationship between BRAF V600E mutation, either with age, gender, or size of the tumor. BRAF V600E immunohistochemical examination can be performed as additional investigation for PTC patients.

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