scholarly journals In vitro Sperm Function Tests and Testicular Biometry for Fertility Prediction in Boar

2017 ◽  
Vol 6 (1) ◽  
pp. 301-307
Author(s):  
Amle M.B. ◽  
◽  
Mundhe S.M. ◽  
Birade H.S. ◽  
◽  
...  
Keyword(s):  
Sperm Function ◽  
Function Tests

The value of in vitro immune function tests in the detection of potential immunotoxicants

1994 ◽  
Vol 8 (5) ◽  
pp. 1021-1025 ◽  
Author(s):  
R.W.R. Crevel ◽  
P. Buckley ◽  
J.A. Robinson
Keyword(s):  
Immune Function ◽  
Function Tests

Evaluation of different sperm function tests as screening methods for male fertilization potential—the value of the sperm migration test

1994 ◽  
Vol 62 (3) ◽  
pp. 591-598 ◽  
Author(s):  
Marinko M. Biljan ◽  
Clare T. Taylor ◽  
Paul R. Manasse ◽  
Edward C. Joughin ◽  
Charles R. Kingsland ◽  
...  
Keyword(s):  
Screening Methods ◽  
Sperm Function ◽  
Migration Test ◽  
Function Tests ◽  
Sperm Migration

Sperm Function Tests

Journal SOGC ◽  
1996 ◽  
Vol 18 (5) ◽  
pp. 435-449
Author(s):  
Ahmed I. Vawda ◽  
Joanne Gunby ◽  
Salim Daya
Keyword(s):  
Sperm Function ◽  
Function Tests

scholarly journals High Throughput Phenotypic Screening of The Human Spermatozoon

Reproduction ◽  
2021 ◽  
Author(s):  
Zoe Claire Johnston ◽  
Franz S Gruber ◽  
Sean Brown ◽  
Neil R Norcross ◽  
Jason R Swedlow ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Large Scale ◽  
Hormonal Contraceptives ◽  
Diagnostic Tools ◽  
Sperm Function ◽  
Phenotypic Screening ◽  
Environmental Chemical ◽  
Potential Applications

Despite recent advances in male reproductive health research, there remain many elements of male (in)fertility where our understanding is incomplete. Consequently, diagnostic tools and treatments for men with sperm dysfunction, other than medically assisted reproduction, are limited. On the other hand, the gaps in our knowledge of the mechanisms which underpin sperm function have hampered the development of male non-hormonal contraceptives. The study of mature spermatozoa is inherently difficult. They are a unique and highly specialised cell type which does not actively transcribe or translate proteins and cannot be cultured for long periods of time or matured in vitro. One, large scale, approach to both increasing understanding of sperm function, and the discovery and development of compounds that can modulate sperm function, is to directly observe responses to compounds with phenotypic screening techniques. These target agnostic approaches can be developed into high-throughput screening platforms with the potential to drastically increase advances in the field. Here we discuss the rationale and development of high-throughput phenotypic screening platforms for mature human spermatozoa, and the multiple potential applications these present, as well as the current limitations and leaps in our understanding and capabilities needed to overcome them. Further development and use of these technologies could lead to the identification of compounds which positively or negatively affect sperm cell motility or function, or novel platforms for toxicology or environmental chemical testing among other applications. Ultimately, each of these potential applications is also likely to increase understanding within the field of sperm biology.

scholarly journals A Potential Relationship Between Estrogen Receptors Polymorphisms, Sperm Function and in vitro Fertilization Success: A Preliminary Study*

2021 ◽  
Vol 75 ◽  
pp. 304-317
Author(s):  
Joanna Talarczyk-Desole ◽  
Mirosław Andrusiewicz ◽  
Małgorzata Chmielewska ◽  
Anna Berger ◽  
Leszek Pawelczyk ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Estrogen Receptors ◽  
Strong Association ◽  
Fertilization Success ◽  
World Health ◽  
Semen Parameters ◽  
Sperm Function ◽  
Allele Distribution ◽  
Vitro Fertilization

Background: Estrogen receptor 1 (ESR1) and 2 (ESR2) play an important role in regulating fertility in the human reproductive system. Polymorphisms of these receptor genes have been implicated in male infertility in both Chinese and Caucasian populations. However, studies have produced inconsistent results. Spermatozoa defects that result in conception deficiencies could be related to estrogens, their receptors, or genes involved in estrogen-related pathways. This study aims to explore the potential association between the ESR1 and the ESR2 polymorphisms in relation to semen parameters of Caucasian males as well as fertilization success. Materials/Methods: A total of 116 males were included in this study. Forty couples underwent conventional in vitro fertilization, while 76 couples were treated by intracytoplasmic sperm injection. Standard semen analyses were performed according to the World Health Organization criteria. Polymerase chain reaction and restriction fragment length polymorphisms were used to determine genotype and allele distributions. Results: A strong association between the ESR1 rs2234693 recognized by PvuII enzyme, genotype/allele distribution and fertilization success was shown. The T allele occurrence was significantly lower in the case of fertilization failure (p = 0.02). Additionally, the TT genotype was absent in the same group (p=0.02). In the case of the remaining analyzed polymorphisms, little to no interdependence of genotype/allele distribution and fertilization success was noted. Conclusions: Apart from ESR1 rs2234693, the study failed to demonstrate that fertilization success was associated with the selected polymorphisms. In most cases, we did not discover a relationship between both estrogen receptors polymorphisms and sperm function.

scholarly journals Binding of boar spermatozoa to oviductal epithelium in vitro in relation to sperm morphology and storage time

Reproduction ◽  
2006 ◽  
Vol 131 (2) ◽  
pp. 311-318 ◽  
Author(s):  
D Waberski ◽  
F Magnus ◽  
F Ardón ◽  
A M Petrunkina ◽  
K F Weitze ◽  
...  
Keyword(s):  
Semen Quality ◽  
Binding Capacity ◽  
Storage Period ◽  
Sperm Function ◽  
Sperm Binding ◽  
Oviductal Epithelium ◽  
Semen Storage ◽  
Boar Semen ◽  
Boar Spermatozoa

In vitro short-term storage of boar semen for up to 72 h before insemination negatively affects fertility, but this often remains undetected during semen quality assessment. One important sperm function is the ability to form the functional sperm reservoir in the oviduct. In the present study, we used the modified oviductal explant assay to study sperm binding to oviductal epithelium in vitro in diluted boar semen stored for 24 or 72 h. First, we determined the kinetics of in vitro sperm binding to oviductal epithelium in relation to co-incubation time of sperm and oviductal tissue pieces. Then, we studied how the binding of sperm to oviductal epithelium was affected by in vitro semen storage and by differences among individual boars. Sperm binding after different incubation times was significantly higher when semen was stored 24 h than after 72-h storage (P < 0.05), and peaked at 30–90 min of incubation. Sperm binding differed between boars (n = 44), and was negatively correlated to the percentage of sperm with cytoplasmic droplets (R = −0.51, P < 0.001). There were no significant changes in motility, acrosome integrity and propidium iodide stainability during the 72-h storage period. However, sperm-binding indices were significantly lower after 72 h in vitro storage than after 24-h storage in sperm from boars with normal semen quality (P < 0.05); in contrast, the binding capacity of sperm from boars with higher percentages of morphologically altered sperm remained at a low level. The sperm-binding capacity of sperm from four of the five boars with known subfertility was lower than the mean binding index minus one standard deviation of the boar population studied here. It is concluded that changes in the plasma membrane associated with in vitro ageing reduce the ability of stored boar sperm to bind to the oviductal epithelium. This study shows the potential of sperm–oviduct binding as a tool to assess both male fertility and changes in sperm function associated with in vitro ageing.

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