scholarly journals The Isolation and Characterization of Proteus mirabilis from Different Clinical Samples

2013 ◽  
Vol 7 (2) ◽  
pp. 24-30
Author(s):  
Wasan W. Al-Bassam ◽  
Abdul-Kareem Al-Kazaz
Keyword(s):  
Antibiotic Resistance ◽  
Nalidixic Acid ◽  
High Resistance ◽  
Proteus Mirabilis ◽  
Urine Samples ◽  
Clinical Samples ◽  
Isolation And Characterization

A total of one hundred five samples were collected from hospitals of Baghdad city during the period from 10/12/2008 to 15/3/2009. These clinical samples included: urine (50) wound swabs (25), sputum (20), and ear swabs (10). These samples were collected from the Baghdad hospital/ Teaching Laboratories, and Al-Yarmook Hospital/ Teaching Laboratories. Twenty six isolates of Proteus mirabilis were characterized according to the morphology and microscopic characteristics, along with the biochemical and confirmatory APi 20 E tests. These isolates were obtained from: urine (19), wound swabs (6), ear swabs (2), and sputum (1). The twenty selected isolates were tested for resistance against ten antibiotics and only urine samples were tested for nalidixic acid and nitrofurantoin resistance. It was shown that there were differences in the antibiotic resistance of isolates. High resistance to nitrofurantoin and ampicillin were found among isolates as (100%) and (75%) respectively while the resistance of Proteus isolates to trimethoprin /sulphamethazol, were (65%). This study also showed that resistance of isolates to gentamicin, ciprofloxacin, ceftazidime, pipracillin, cefotaxime, nalidixic acid azteronam, imipenem and amikacin were (50, 40, 40, 40, 35,27, 20,15, 5)% respectively.

scholarly journals Isolation and Characterization of Novel Human Parechovirus from Clinical Samples

2007 ◽  
Vol 13 (6) ◽  
pp. 889-895 ◽  
Author(s):  
Kanako Watanabe ◽  
Masayasu Oie ◽  
Masaya Higuchi ◽  
Makoto Nishikawa ◽  
Masahiro Fujii
Keyword(s):  
Clinical Samples ◽  
Human Parechovirus ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of bacteriophages that infect Citrobacter rodentium, a model pathogen for investigating human intestinal diseases

2018 ◽  
Author(s):  
Carolina M. Mizuno ◽  
Tiffany Luong ◽  
Robert Cedarstrom ◽  
Mart Krupovic ◽  
Laurent Debarbieux ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Phage Therapy ◽  
Limit Of Detection ◽  
Lytic Cycle ◽  
Citrobacter Rodentium ◽  
E Coli ◽  
Isolation And Characterization ◽  
A New Species

AbstractEnteropathogenic Escherichia coli (EPEC) is a major etiology for diarrheal diseases among children. Antibiotics, when used appropriately, are effective; however, their overuse and misuse has led to the rise of antibiotic resistance worldwide. Thus, there are renewed efforts into the development of phage therapy. Due to the drawbacks of EPEC in vivo models, a surrogate is the mouse-restricted gut pathgoen Citrobacter rodentium. In this study, two new phages CrRp3 and CrRp10, which infect C. rodentium, were isolated and characterized. CrRp3 was found to be a new species within the genus Vectrevirus and CrRp10 is a new strain within the genus Tequatrovirus. Neither phage carries known genes associated with bacterial virulence, antibiotic resistance, or lysogeny. CrRp3 and CrRp10 appear to have independently evolved from E. coli phages. CrRp3 appears to be the more ‘potent’ being 24x more likely to find a host cell and has a shorter lytic cycle, while CrRp10 at MOI 0.001 was able to maintain bacterial density below the limit of detection after 18 h. We found that hypoxia (5% O2 and 5% CO2) inhibited CrRp3 ability to reverse exponential bacterial growth. It is unclear whether the subtle characteristic differences between CrRp3 and CrRp10 will influence treatment efficacy in future phage therapy in vivo investigations.

scholarly journals Integrated characterization of SARS-CoV-2 genome, microbiome, antibiotic resistance and host response from single throat swabs

2020 ◽  
Author(s):  
Bo Lu ◽  
Yi Yan ◽  
Liting Dong ◽  
Lingling Han ◽  
Yawei Liu ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Severe Acute Respiratory Syndrome ◽  
Host Response ◽  
Comprehensive Analysis ◽  
Clinical Samples ◽  
Full Genome ◽  
Rna Seq ◽  
Sequencing Data ◽  
Genome Information

AbstractThe ongoing coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, poses a severe threat to humanity. Rapid and comprehensive analysis of both pathogen and host sequencing data is critical to track infection and inform therapies. In this study, we performed unbiased metatranscriptomic analysis of clinical samples from COVID-19 patients using a newly-developed RNA-seq library construction method (TRACE-seq), which utilizes tagmentation activity of Tn5 on RNA/DNA hybrids. This approach avoids the laborious and time-consuming steps in traditional RNA-seq procedure, and hence is fast, sensitive and convenient. We demonstrated that TRACE-seq allowed integrated characterization of full genome information of SARS-CoV-2, putative pathogens causing coinfection, antibiotic resistance and host response from single throat swabs. We believe that the integrated information will deepen our understanding of pathogenesis and improve diagnostic accuracy for infectious diseases.

scholarly journals ISOLATION AND CHARACTERIZATION OF ESCHERICHIA COLI FROM BUFFALO CALVES IN SOME SELECTED AREAS OF BANGLADESH

1970 ◽  
Vol 8 (1) ◽  
pp. 23-26 ◽  
Author(s):  
SK Paul ◽  
MSR Khan ◽  
MA Rashid ◽  
J Hassan ◽  
SMS Mahmud
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Rectal Swab ◽  
Antibiotic Sensitivity ◽  
Buffalo Calves ◽  
E Coli ◽  
Isolation And Characterization ◽  
Sensitivity Pattern ◽  
Highly Sensitive

The research works was conducted with a view to isolate and identify the Escherichia coli (E. coli) organism from diarrhoeic cases of buffalo reared in selected areas of Bangladesh as well the prevalence and antibiotic sensitivity pattern of the isolated E. coli in the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh-2202 during the period from April 2008 to May 2009. A total of 50 rectal swab samples were collected from 4 different places namely Haluaghat and Boira of Mymensingh, Madupur of Tangail and Kazipur of Sirajgonj districts. The samples were aseptically carried to the laboratory of the Department of Microbiology and Hygiene and subjected to different cultural, morphological and biochemical examinations. Upon cultural, morphological and biochemical examinations 23 (45%) samples were found to be positive for E. coli. The highest prevalence was found in Haluaghat, Mymensingh (53.33%) and the lowest (40.00%) in Boira, Mymensingh and Kazipur, Sirajganj. Antibiogram study revealed that the isolated E. coli was highly sensitive to Enrofloxacin and Ciprofloxacin, moderately sensitive to Cefalexin and Amoxicillin, and resistant to Nalidixic acid and Erythromycin. DOI = 10.3329/bjvm.v8i1.7398 Bangl. J. Vet. Med. (2010). 8(1): 23-26

Isolation and characterization of antibiotic-resistance plasmids in thermophilic bacilli

1985 ◽  
Vol 31 (4) ◽  
pp. 339-345 ◽  
Author(s):  
Takayuki Hoshino ◽  
Takayuki Ikeda ◽  
Hiroyuki Narushima ◽  
Noboru Tomizuka
Keyword(s):  
Antibiotic Resistance ◽  
Southern Hybridization ◽  
Tetracycline Resistance ◽  
Restriction Endonuclease Analysis ◽  
Kanamycin Resistance ◽  
Isolation And Characterization ◽  
Thermophilic Bacilli ◽  
Resistance Plasmids ◽  
Endonuclease Analysis

Four antibiotic-resistance plasmids isolated from thermophilic bacilli were characterized in detail. Three tetracycline-resistance (Tcr) plasmids were designated as pTHT9 (7.7 kilobases (kb)), pTHT15 (4.5 kb) and pTHT22 (8.4 kb). From the results of restriction endonuclease analysis and the subsequent Southern hybridization, these were found to possess extensive genetic homology in the regions that include the replication origin and the Tcr gene. Detailed restriction maps of the smallest Tcr plasmid pTHT15 and a kanamycin-resistance (Kmr) plasmid pTHN1 (4.8 kb) were constructed. The positions of antibiotic-resistance loci and regions essential for plasmid replication were determined by cloning plasmid fragments in Bacillus subtilis. These four plasmids were found to replicate and express the resistance genes stably in both B. subtilis and B. stearothermophilus.

scholarly journals Detection of Salmonella spp. in Retail Raw Food Samples from Vietnam and Characterization of Their Antibiotic Resistance

2007 ◽  
Vol 73 (21) ◽  
pp. 6885-6890 ◽  
Author(s):  
Thi Thu Hao Van ◽  
George Moutafis ◽  
Taghrid Istivan ◽  
Linh Thuoc Tran ◽  
Peter J. Coloe
Keyword(s):  
Antibiotic Resistance ◽  
Nalidixic Acid ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Antimicrobial Agents ◽  
Genomic Island ◽  
Antibiotic Resistance Gene ◽  
Food Samples ◽  
Salmonella Spp

ABSTRACT A study was conducted to examine the levels of Salmonella spp. contamination in raw food samples, including chicken, beef, pork, and shellfish, from Vietnam and to determine their antibiotic resistance characteristics. A total of 180 samples were collected and examined for the presence of Salmonella spp., yielding 91 Salmonella isolates. Sixty-one percent of meat and 18% of shellfish samples were contaminated with Salmonella spp. Susceptibility of all isolates to a variety of antimicrobial agents was tested, and resistance to tetracycline, ampicillin/amoxicillin, nalidixic acid, sulfafurazole, and streptomycin was found in 40.7%, 22.0%, 18.7%, 16.5%, and 14.3% of the isolates, respectively. Resistance to enrofloxacin, trimethoprim, chloramphenicol, kanamycin, and gentamicin was also detected (8.8 to 2.2%). About half (50.5%) of the isolates were resistant to at least one antibiotic, and multiresistant Salmonella isolates, resistant to at least three different classes of antibiotics, were isolated from all food types. One isolate from chicken (serovar Albany) contained a variant of the Salmonella genomic island 1 antibiotic resistance gene cluster. The results show that antibiotic resistance in Salmonella spp. in raw food samples from Vietnam is significant.

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