Isolation and Characterization of Novel Human Parechovirus from Clinical Samples

A total of one hundred five samples were collected from hospitals of Baghdad city during the period from 10/12/2008 to 15/3/2009. These clinical samples included: urine (50) wound swabs (25), sputum (20), and ear swabs (10). These samples were collected from the Baghdad hospital/ Teaching Laboratories, and Al-Yarmook Hospital/ Teaching Laboratories. Twenty six isolates of Proteus mirabilis were characterized according to the morphology and microscopic characteristics, along with the biochemical and confirmatory APi 20 E tests. These isolates were obtained from: urine (19), wound swabs (6), ear swabs (2), and sputum (1). The twenty selected isolates were tested for resistance against ten antibiotics and only urine samples were tested for nalidixic acid and nitrofurantoin resistance. It was shown that there were differences in the antibiotic resistance of isolates. High resistance to nitrofurantoin and ampicillin were found among isolates as (100%) and (75%) respectively while the resistance of Proteus isolates to trimethoprin /sulphamethazol, were (65%). This study also showed that resistance of isolates to gentamicin, ciprofloxacin, ceftazidime, pipracillin, cefotaxime, nalidixic acid azteronam, imipenem and amikacin were (50, 40, 40, 40, 35,27, 20,15, 5)% respectively.

Download Full-text

Isolation and characterization of bacteriophages from wastewater sources on Enterococcus spp. isolated from clinical samples

Iranian Journal of Microbiology ◽

10.18502/ijm.v13i5.7434 ◽

2021 ◽

Author(s):

Yara Elahi ◽

Jamileh Nowroozi ◽

Ramin Mazaheri Nezhad Fard

Keyword(s):

Multidrug Resistant ◽

Eukaryotic Cells ◽

Clinical Samples ◽

Streptococcal Infections ◽

Bacterial Isolates ◽

Streptococcus Dysgalactiae ◽

Antibiotic Resistant ◽

Isolation And Characterization ◽

Enterococcus Spp

Background and Objectives: In recent decades, enterococcal resistance to antimicrobials has greatly increased. Further- more, these chemicals include several side effects on the patients. Since no reports are available of the bacteriophages' effects on eukaryotic cells, they can be good solutions for multidrug-resistant bacterial problems. Therefore, the major aim of this study was to isolate bacteriophages from wastewaters on clinical antibiotic-resistant enterococci. Materials and Methods: Clinical bacteria were isolated, then enterococcal isolates were identified using different methods. The antibiotic resistance scheme of the enterococcal isolates was assessed. The bacterial isolates were exposed to wastewa- ter samples containing potential bacteriophages. Technically, isolated bacteriophages were studied by electron microscopy. Results: Isolated bacteria were verified as Enterococcus faecium. Results showed that bacteriophages could easily be isolat- ed from wastewater sources. The isolated bacteriophages were effective on E. faecium as well as Streptococcus dysgalactiae. Furthermore, these bacteriophages were challenged with five other bacteria (ATCC) with no visible effects. In general, the isolated bacteriophages belonged to the Myoviridae, Siphoviridae, and Inoviridae families. Conclusion: Further studies on bacteriophages and their efficacy on enterococcal strains could increase the treatment possi- bility of enterococcal infections. Due to these bacteriophages' effects on Streptococcus strains, bacteriophages may be used to treat streptococcal infections as well.

Download Full-text

Characterization of Aminoglycoside Modifying Enzymes Producing E. coli and Klebsiella pneumoniae Clinical Isolates

Journal of Pure and Applied Microbiology ◽

10.22207/jpam.15.4.68 ◽

2021 ◽

Author(s):

Eman El-Gebaly ◽

Mohammed Farrag ◽

Tarek Dishisha ◽

Walid Bakeer

Keyword(s):

16S Rrna ◽

Clinical Isolates ◽

Diffusion Method ◽

Clinical Samples ◽

Aminoglycoside Resistance ◽

Efficient Treatment ◽

E Coli ◽

Isolation And Characterization ◽

Antimicrobial Resistance Gene

Antimicrobial resistance gene profile characterization and dissemination offer useful detail on the possible challenge in treating bacteria. The development of aminoglycoside modifying enzymes (AMEs) is considered as the primary mechanism of resistance to aminoglycosides, in addition to the 16S rRNA methylases. This study aimed at isolation and characterization of aminoglycosides resistant clinical isolates of enterobacteriaceae family from different clinical samples. Over a period of 24 months, thirty samples were collected and 49 clinical isolates of E. coli [n=25], Klebsiella [n=13], Enterobacter species (n=7) and Proteus species (n=4) were isolated from Egyptian clinical laboratories. The identities of the cultures were confirmed following standard microbiological procedures. Resistance of the isolates to aminoglycosides was determined by the disc diffusion method and isolates with highest resistance (n=9) were selected and investigated for 16S rRNA methylase and AMES encoding genes by polymerase chain reaction (PCR) and sequencing. In general, aminoglycoside resistance was found in 95% of the isolates; the isolates displayed the highest rate of resistance to netilmicin (75%) and kanamycin (55%), while resistance to gentamycin (18%) and tobramycin (16%) was low. A total of 9 isolates have the highest aminoglycoside resistant rate, showed the highest appearance for aac(6′)-Ib as well as ant (3″)-Ia resistant genes, with aac (3)-II (44%) and ant (4′)-IIb (34%) following closely. The high prevalence of AMEs observed among resistant isolates in this study suggests the urgent need for more efficient treatment designs to mitigate the selection burden as well as improved care of patients who have been infected with these drug-resistant organisms.

Download Full-text

First Report of Isolation and Characterization of Aurantimonas altamirensis from Clinical Samples

Journal of Clinical Microbiology ◽

10.1128/jcm.00337-08 ◽

2008 ◽

Vol 46 (7) ◽

pp. 2435-2437 ◽

Cited By ~ 15

Author(s):

M.-L. Luong ◽

S. Bekal ◽

D. C. Vinh ◽

D. Lauzon ◽

V. Leung ◽

...

Keyword(s):

Clinical Samples ◽

First Report ◽

Isolation And Characterization

Download Full-text

First Record of Isolation and Characterization of Methicillin ResistantStaphylococcus lugdunensisfrom Clinical Samples in Iraq

BioMed Research International ◽

10.1155/2014/736259 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 3

Author(s):

Alaa H. Al-Charrakh ◽

Mohammed H. Obayes

Keyword(s):

Molecular Detection ◽

Susceptibility Testing ◽

First Record ◽

Clinical Samples ◽

Antibiotic Susceptibility Testing ◽

Coagulase Negative Staphylococci ◽

Staphylococcus Lugdunensis ◽

Isolation And Characterization ◽

Resistance Rates

This study was conducted to determine the frequency ofStaphylococcus lugdunensisin different clinical samples. Out of 690 clinical samples, a total of 178 coagulase negative staphylococci (CoNS) isolates were recovered. CoNS were identified as 10 different species; 22 isolates belonged toStaphylococcus lugdunensis. Two specific genes forS. lugdunensiswere used (tanAgene andfblgene) to confirm identification. Both of these specific genes were detected in 15 (68.1%) of 22 isolates that were identified phenotypically. The results of oxacillin MIC showed that 7 of the 15 (46.6%)S. lugdunensisisolates were oxacillin resistant. The antibiotic susceptibility testing against 16 antibiotics showed that resistance rates were variable towards these antibiotics. Eight of fifteenS. lugdunensisisolates (53.3%) wereβ-lactamase producer. Results of molecular detection ofmecAgene found thatmecAgene was detected in 6 (40%) of 15S. lugdunensis. All of these 6 isolates (S1, S2, S3, S4, S5, and S6) were resistant to oxacillin. One isolate (S7) was resistant to oxacillin butmecAwas not detected in this isolate. This study is a first record of isolation and characterization of methicillin resistantS. lugdunensis(MRSL) from clinical samples in Iraq.

Download Full-text