Use of a polymerase chain reaction assay to detect bovine herpesvirus type 2 DNA in skin lesions from cattle suspected to have pseudo-lumpy skin disease

2003 ◽  
Vol 222 (10) ◽  
pp. 1404-1407 ◽  
Author(s):  
Jean M. d'Offay ◽  
James G. Floyd ◽  
Richard Eberle ◽  
Jerry T. Saliki ◽  
Kenny V. Brock ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Skin Disease ◽  
Polymerase Chain Reaction Assay ◽  
Bovine Herpesvirus ◽  
Skin Lesions ◽  
Chain Reaction ◽  
Lumpy Skin Disease ◽  
Herpesvirus Type ◽  
Polymerase Chain

scholarly journals Molecular detection of lumpy skin disease virus in cattle by polymerase chain reaction in Iraq

2016 ◽  
Vol 40 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Khitam Mahdi Mhemid
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Skin Disease ◽  
Age Groups ◽  
Conventional Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Lumpy Skin Disease ◽  
Blood Samples ◽  
Lumpy Skin Disease Virus ◽  
Polymerase Chain

     This study is conducted to detect lumpy skin disease virus in Babylon, Al-Qadysia and Al-Muthana governorate during autumn 2014 using conventional polymerase chain reaction. A total of 150 specimens: 50 whole blood samples, 50 skin nodular biopsies and 50 tick samples were collected from infected animals of different breeds, genders and ages during lumpy skin disease outbreak. The results revealed that 104 cases (69.33%) were positive for lumpy skin disease virus by using polymerase chain reaction, with significant (P<0.05) differences between positive and negative cases. Out of 50 blood samples, 22 cases (44%) were positive for lumpy skin disease virus. The nodular skin samples collected from slaughtered animals showed 36 positive cases (72%), whereas 46 tick samples (92%) were positive for the disease, with significant (P<0.05) difference among them. According to gender, the finding showed significant results of lumpy skin disease in females (78.78%). It was recorded that higher percentage of positive cases was found in Friesian cattle (100%), crossbreed (73.58%) while native breed was (50.76%) with significant (P<0.05) difference among them. Regarding age groups, the results showed that all ages were susceptible to lumpy skin disease and significantly not different.

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