scholarly journals Comparison between bone alkaline phosphatase immunoassay and electrophoresis technique in hemodialysis patients

2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Neda Milinković ◽  
Marija Sarić Matutinović ◽  
Svetlana Pejanović ◽  
Svetlana Ignjatović
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Alkaline Phosphatase ◽  
Hemodialysis Patients ◽  
Cusum Test ◽  
Agar Gel ◽  
Agar Gel Electrophoresis ◽  
Automated Methods ◽  
Electrophoresis Technique ◽  
Total Alkaline ◽  
Good Agreement

Summary Background Problem of the variability between the different methods using for bone alkaline phosphatase (bALP) determination greately influences the clinical significance of bALP as direct marker of bone metabolism. The aim of this study was to compare immunoassay with electrophoresis technique for bALP determination. Methods We measured bALP in 71 patients on hemodialysis with agar gel electrophoresis (ISO-PAL, SEBIA) and immunoassay (OSTASE, Beckman Coulter). Results The analyzed methods showed significant correlation (Spearman’s rho: 0.776, P < 0.01), but we found statistically significant (P < 0.01) positive bias (27%) for the results measured by immunoassay. In support of this, using electrophoresis technique we have detected presence of the intestinal isoenzymes of alkaline phosphatase in 55% of patients with median value of 30% of the total alkaline phoshatase and presence of liver-2 alkaline phosphatase isoform in 42% of patients with median value of 16.6%. The Kendall’s W of 0.787 (P<0.0001) revealed significant concordance between two analysed methods. Cusum test showed no significant deviation from linearity (P=0.850). Conclusions Despite good agreement between immunoassay methods and electrophoresis technique for bALP determination, interchangeability between these two methods is questionable. Although immunoassays are increasingly used, as fully automated methods, in a large number of laboratories and become routine methods for bALP determination, it should be beared in mind, besides various interferences, also the heterogeneity of the bALP itself, especially in patients on hemodialysis.

A New Alkaline Phosphatase Isoenzyme Abnormality

1984 ◽  
Vol 21 (5) ◽  
pp. 352-356
Author(s):  
P D Mayne ◽  
A Ying Foo ◽  
Rosalind Michelson ◽  
I Z Kovar ◽  
S B Rosalki
Keyword(s):  
Alkaline Phosphatase ◽  
Gel Electrophoresis ◽  
Incidental Finding ◽  
Male Infant ◽  
Normal Liver ◽  
Bone Alkaline Phosphatase ◽  
Total Alkaline Phosphatase ◽  
Alkaline Phosphatase Staining ◽  
Alkaline Phosphatase Isoenzyme ◽  
Total Alkaline

We report on a new alkaline phosphatase isoenzyme abnormality occurring as an incidental finding in a male infant aged 4 months. Isoenzyme electrophoresis on cellulose acetate showed a prominent, diffuse alkaline phosphatase staining band in the α1-globulin position together with a second band in the α2/β region and minor ‘trailing’ in the intermediate α2 region. Normal liver and bone alkaline phosphatase were absent and intestinal phosphatase was not detected. On acrylamide gel electrophoresis a marked origin band was detectable suggesting the presence of high molecular weight enzyme. In addition, a series of compact bands in the α2/β position was present cathodal to the usual liver and bone isoenzymes. Total alkaline phosphatase activity was marginally elevated and was heat labile, L-phenylalanine resistant and partially L-homoarginine and L-leucine sensitive.

Bone Alkaline Phosphatase besides Intact Parathyroid Hormone in Hemodialysis Patients – Any Advantage?

2005 ◽  
Vol 101 (3) ◽  
pp. c122-c127 ◽  
Author(s):  
Cristina Jorge ◽  
Célia Gil ◽  
Marília Possante ◽  
Eugénia Silva ◽  
Rui Andrade ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Bone Alkaline Phosphatase ◽  
Hemodialysis Patients ◽  
Intact Parathyroid Hormone

Bone to Total Alkaline Phosphatase Ratios Improve Sensitivity and Specificity of Bone Alkaline Phosphatase Immunoassays

1997 ◽  
Vol 30 (8) ◽  
pp. 625-629 ◽  
Author(s):  
Lori J Sokoll ◽  
Martin H Kroll ◽  
Michael A Levine ◽  
F.Fred Poordad ◽  
Daniel W Chan
Keyword(s):  
Alkaline Phosphatase ◽  
Sensitivity And Specificity ◽  
Bone Alkaline Phosphatase ◽  
Total Alkaline Phosphatase ◽  
Total Alkaline

scholarly journals Multiple forms of alkaline phosphatase in plasma of hemodialysis patients

1991 ◽  
Vol 37 (6) ◽  
pp. 815-820 ◽  
Author(s):  
L Tibi ◽  
S C Chhabra ◽  
V M Sweeting ◽  
R J Winney ◽  
A F Smith
Keyword(s):  
Alkaline Phosphatase ◽  
Total Activity ◽  
Bone Alkaline Phosphatase ◽  
Intestinal Alkaline Phosphatase ◽  
Total Alkaline Phosphatase ◽  
Gamma Glutamyltransferase ◽  
Biochemical Indices ◽  
Increased Activity ◽  
Separate Measurement ◽  
Total Alkaline

Abstract We used quantitative assays to measure the activity of the bone, liver, and intestinal forms of alkaline phosphatase in plasma in 75 patients with endstage chronic renal failure undergoing hemodialysis. The results were correlated with radiological and other biochemical indices of bone disease and with biochemical indices of liver disease. The total activity of alkaline phosphatase in plasma increased in 28 patients. In 10 of these patients, nine of whom had increased activity of gamma-glutamyltransferase in plasma, the increase in total activity of alkaline phosphatase was from the liver isoenzyme alone (nine patients) or from the liver and bone isoenzymes together (one patient). Intestinal alkaline phosphatase in plasma, although greater than 23 U/L in eight patients, was solely responsible for the increase in total alkaline phosphatase in one patient (who had normal gamma-glutamyltransferase). Bone alkaline phosphatase in plasma was increased in 25 patients, seven of whom had normal total alkaline phosphatase, and was closely correlated (r = 0.78) with osteocalcin concentration in plasma, which was increased in a much greater proportion of patients (99%). Both total and bone alkaline phosphatase were correlated with parathyrin in plasma (r = 0.46 and 0.50, respectively) and with osteocalcin (r = 0.60 and 0.78, respectively). Osteocalcin and bone alkaline phosphatase, but not parathyrin, decreased with age, implying that the skeletal response to parathyrin may be age dependent. In patients with increased total alkaline phosphatase undergoing hemodialysis, the concurrent measurement of gamma-glutamyltransferase may help identify whether the enzyme increase originates from the liver or bone, but this approach wrongly identified the source of the increase in three of 28 patients. Therefore, we recommend a separate measurement of the bone isoenzyme of alkaline phosphatase.

An Evaluation of 1–84 PTH Measurement in Relation to Bone Alkaline Phosphatase and Bone Gla Protein in Hemodialysis Patients

2004 ◽  
Vol 94 (2) ◽  
pp. c29-c32 ◽  
Author(s):  
Naoko Miwa ◽  
Kosaku Nitta ◽  
Naoki Kimata ◽  
Yoshihiko Watanabe ◽  
Koichi Suzuki ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Alkaline Phosphatase ◽  
Hemodialysis Patients ◽  
Bone Gla Protein

Bone Alkaline Phosphatase in Rheumatic Diseases

1995 ◽  
Vol 32 (4) ◽  
pp. 379-384 ◽  
Author(s):  
Christine Beyeler ◽  
Rosamonde E Banks ◽  
Douglas Thompson ◽  
Mary A Forbes ◽  
Edward H Cooper ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Disease Activity ◽  
Rheumatic Diseases ◽  
Active Rheumatoid Arthritis ◽  
Bone Alkaline Phosphatase ◽  
Plasma Viscosity ◽  
Inflammatory Rheumatic Diseases ◽  
Total Alkaline Phosphatase ◽  
Glutamyl Transferase ◽  
Total Alkaline

A double monoclonal immunoradiometric assay specific for bone alkaline phosphatase (BAP) was used to determine whether the raised total alkaline phosphatase (TAP) often found in patients with active rheumatoid arthritis (RA) and ankylosing spondylitis (AS) is derived from bone or liver. Fifty-eight patients with RA were compared to 14 with AS and 14 with non-inflammatory rheumatic diseases (NI). None had clinical liver disease and only one had a slightly elevated aspartate transaminase activity. Elevated BAP concentrations were found in seven patients (5 RA, 1 AS, 1 NI), only two of whom also had abnormal TAP. Abnormal TAP activities were found in only three patients (all RA). BAP did not correlate with disease activity in RA or AS. In contrast, TAP correlated with disease activity (assessed by plasma viscosity) in RA ( P < 0·02) and AS ( P < 0·002) and γ-glutamyl transferase (GGT) also correlated with plasma viscosity in RA ( P < 0·01). Both TAP and BAP were significantly correlated with GGT in RA ( P < 0·001 and P < 0·02, respectively). These findings are discussed, together with possible reasons for the conflicting nature of some of the observations.

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