An Evaluation of 1–84 PTH Measurement in Relation to Bone Alkaline Phosphatase and Bone Gla Protein in Hemodialysis Patients

2004 ◽  
Vol 94 (2) ◽  
pp. c29-c32 ◽  
Author(s):  
Naoko Miwa ◽  
Kosaku Nitta ◽  
Naoki Kimata ◽  
Yoshihiko Watanabe ◽  
Koichi Suzuki ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Alkaline Phosphatase ◽  
Hemodialysis Patients ◽  
Bone Gla Protein

scholarly journals Comparison between bone alkaline phosphatase immunoassay and electrophoresis technique in hemodialysis patients

2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Neda Milinković ◽  
Marija Sarić Matutinović ◽  
Svetlana Pejanović ◽  
Svetlana Ignjatović
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Alkaline Phosphatase ◽  
Hemodialysis Patients ◽  
Cusum Test ◽  
Agar Gel ◽  
Agar Gel Electrophoresis ◽  
Automated Methods ◽  
Electrophoresis Technique ◽  
Total Alkaline ◽  
Good Agreement

Summary Background Problem of the variability between the different methods using for bone alkaline phosphatase (bALP) determination greately influences the clinical significance of bALP as direct marker of bone metabolism. The aim of this study was to compare immunoassay with electrophoresis technique for bALP determination. Methods We measured bALP in 71 patients on hemodialysis with agar gel electrophoresis (ISO-PAL, SEBIA) and immunoassay (OSTASE, Beckman Coulter). Results The analyzed methods showed significant correlation (Spearman’s rho: 0.776, P < 0.01), but we found statistically significant (P < 0.01) positive bias (27%) for the results measured by immunoassay. In support of this, using electrophoresis technique we have detected presence of the intestinal isoenzymes of alkaline phosphatase in 55% of patients with median value of 30% of the total alkaline phoshatase and presence of liver-2 alkaline phosphatase isoform in 42% of patients with median value of 16.6%. The Kendall’s W of 0.787 (P<0.0001) revealed significant concordance between two analysed methods. Cusum test showed no significant deviation from linearity (P=0.850). Conclusions Despite good agreement between immunoassay methods and electrophoresis technique for bALP determination, interchangeability between these two methods is questionable. Although immunoassays are increasingly used, as fully automated methods, in a large number of laboratories and become routine methods for bALP determination, it should be beared in mind, besides various interferences, also the heterogeneity of the bALP itself, especially in patients on hemodialysis.

Bone Alkaline Phosphatase besides Intact Parathyroid Hormone in Hemodialysis Patients – Any Advantage?

2005 ◽  
Vol 101 (3) ◽  
pp. c122-c127 ◽  
Author(s):  
Cristina Jorge ◽  
Célia Gil ◽  
Marília Possante ◽  
Eugénia Silva ◽  
Rui Andrade ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Bone Alkaline Phosphatase ◽  
Hemodialysis Patients ◽  
Intact Parathyroid Hormone

Concurrent Assays of Circulating Bone Gla-Protein and Bone Alkaline Phosphatase: Effects of Sex, Age, and Metabolic Bone Disease*

1988 ◽  
Vol 66 (5) ◽  
pp. 951-957 ◽  
Author(s):  
RALPH J. DUDA ◽  
JOHN F. O’BRIEN ◽  
JERRY A. KATZMANN ◽  
JAMES M. PETERSON ◽  
KENNETH G. MANN ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Disease ◽  
Metabolic Bone Disease ◽  
Bone Alkaline Phosphatase ◽  
Bone Gla Protein ◽  
Metabolic Bone

scholarly journals Plasma total versus bone alkaline phosphatase as markers of bone turnover in hemodialysis patients.

1996 ◽  
Vol 7 (3) ◽  
pp. 506-512
Author(s):  
P Ureña ◽  
M Hruby ◽  
A Ferreira ◽  
K S Ang ◽  
M C de Vernejoul
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Formation ◽  
Bone Turnover ◽  
Bone Disease ◽  
Formation Rate ◽  
Bone Alkaline Phosphatase ◽  
Hemodialysis Patients ◽  
High Turnover ◽  
Bone Formation Rate ◽  
Markers Of Bone Turnover

Plasma total versus bone alkaline phosphatase as markers of bone turnover in hemodialysis patients. Plasma bone-specific alkaline phosphatase (bAP) has been demonstrated to be more reliable than total alkaline phosphatases (tAP) in providing information about bone turnover in patients with metabolic bone diseases. This study surveyed 42 hemodialysis patients who underwent a systematic transiliac bone biopsy for histomorphometry study. Plasma bAP was determined by using a new immunoassay (Tandem-R Ostase, Hybritech, Liège, Belgium). Plasma bAP values were compared with those of two other plasma markers of bone metabolism, namely tAP and intact parathyroid hormone (iPTH), for the correlations with bone histomorphometric parameters. Patients with high-turnover bone disease (HTBD) (N = 32) had significantly higher plasma bAP levels than patients with normal or low bone turnover (N/LTBD) (N = 10) (66.9 +/- 63.5 ng/mL versus 10.8 +/- 4.2 ng/mL, respectively). Bone formation and resorption were highly correlated in these patients, and plasma bAP levels were positively correlated with bone resorption parameters, including osteoclast surface (r = 0.39, P < 0.0001) and osteoclast number/mm2 (r = 0.36, P < 0.001), and with bone formation parameters, osteoblast surface (r = 0.50, P < 0.005), and bone formation rate (r = 0.91, P < 0.0001). The bone formation rate was better correlated with plasma bAP levels than with either plasma tAP or iPTH concentrations. Plasma bAP level equal or higher than 20 ng/mL, either alone or combined with plasma iPTH of 200 pg/mL, had the highest sensitivity, specificity, and predictability values for the diagnosis of high-turnover bone disease, and formally excluded patients with normal or LTBD. In conclusion, plasma bAP can be measured with a reliable immunoassay in hemodialysis patients. It represents a highly sensitive and specific biochemical marker of skeletal remodeling in these patients. Therefore, both serum iPTH and bAP are complementary in diagnoses of the type of renal osteodystrophy.

Higher serum bone alkaline phosphatase as a predictor of mortality in male hemodialysis patients

Life Sciences ◽  
2012 ◽  
Vol 90 (5-6) ◽  
pp. 212-218 ◽  
Author(s):  
Ikue Kobayashi ◽  
Kaori Shidara ◽  
Senji Okuno ◽  
Shinsuke Yamada ◽  
Yasuo Imanishi ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Alkaline Phosphatase ◽  
Hemodialysis Patients ◽  
Serum Bone Alkaline Phosphatase

scholarly journals New insights into vitamin D regulation: is there a role for alkaline phosphatase?

2021 ◽  
Author(s):  
G. Bellastella ◽  
L. Scappaticcio ◽  
M. Longo ◽  
R. Carotenuto ◽  
C. Carbone ◽  
...  
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase ◽  
Liver Function ◽  
Significant Negative Correlation ◽  
Liver Function Tests ◽  
Bone Alkaline Phosphatase ◽  
Gamma Glutamyl Transpeptidase ◽  
Function Tests ◽  
Group 2 ◽  
Group 1

Abstract Purpose The diagnosis of vitamin D deficiency is based on the determination of total plasma 25-hydroxyvitamin D (25-OHD) concentrations, but the regulation of vitamin D 25-hydroxylation is not a major consideration and very little information is available on this activity. To check what factors could interfere with the activity of vitamin D-25-hydroxylase and thus alter the 25-OHD concentrations, we looked for potential correlations between 25-OHD and results of liver function tests in healthy adults. Methods This single-centre study was retrospective and consisted of evaluating the correlations between 25-OHD and the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), and bone alkaline phosphatase (BALP) in 349 healthy subjects aged from 18 to 65 years. In particular, in Group 1 (n = 119), we looked for correlations between 25OHD and all liver function tests and in Group 2 (n = 230) the correlation between 25OHD and BALP. Results In Group 1, we found no correlation between 25OHD and AST (r =  − 0.03; p = 0.8), ALT (r =  − 0.02; p = 0.91), GGT (r =  − 0.08; p = 0.68), direct bilirubin (r =  − 0.02; p = 0.89), indirect bilirubin (r =  − 0.24; p = 0.21), and total bilirubin (r =  − 0.24; p = 0.21) but one between 25OHD and ALP (r =  − 0.2; p = 0.007); in Group 2, we found a significant negative correlation between 25-OHD and BALP (r =  − 0.2; p = 0.0008). Conclusions The correlations that we found suggest that ALP and BALP might be involved in the regulation of vitamin D-25-hydroxylase activity, but further studies are mandatory to confirm our assumptions.

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