scholarly journals Molecular diagnostic of Ureaplasma urealyticum presence and tetracycline resistance in urine samples

2021 ◽  
Vol 29 (1) ◽  
pp. 43-51
Author(s):  
Mihaela Laura Vica ◽  
Horea Vladi Matei ◽  
Agnes Katona ◽  
Aida Puia ◽  
Cosmin Adrian Teodoru
Keyword(s):  
Ureaplasma Urealyticum ◽  
Rapid Development ◽  
Simultaneous Detection ◽  
Tetracycline Resistance ◽  
The United States ◽  
Urine Samples ◽  
Molecular Diagnostic ◽  
Genetic Determinants ◽  
Sexually Transmitted ◽  
Urease Gene

Abstract Sexually transmitted infections (STIs) are among the most common infections in Romania. Infection with Ureaplasma urelyticum is one of the major causes of STIs and can cause serious complications. Although tetracycline is the drug commonly used to treat infections caused by U. urealyticum, several studies indicate the emergence and rapid development of strains resistant to these antibiotics in the United States or Europe. Tetracycline resistance in bacteria is encoded by a number of different genetic determinants but in mycoplasmas the only tetracycline resistance determinant that has been reported is the tetM gene. Tetracycline resistance among Ureaplasma spp. is associated with the presence of the horizontally acquired tetM resistance gene. Our study on bacterial DNA aimed to determine the presence of tetracycline-resistant U. urealyticum strains, by identifying the presence of the tetM gene. We used first void urine samples from 622 STI-suspected subjects. DNA was extracted, purified and amplified via PCR for the simultaneous detection of 6 STIs. 68 patients were diagnosed with U. urealyticum. DNA obtained from these samples was amplified using the tetM gene and U. urealyticum - specific urease gene primers. The urease gene was amplified in all samples, confirming the presence of U. urealyticum. The tetM gene was amplified in 2 samples considered tetracycline-resistant strains. The study confirmed the presence of U. urealyticum strains resistant to tetracycline in Romania. The employed technique can produce quick results both for U. urealyticum detection and determination of its resistance to tetracycline using a single easy-to-collect biological sample.

scholarly journals Distribution of sexually transmitted diseases in a group of symptomatic male patients using urine samples and PCR technique / Distribuția bolilor cu transmitere sexuală într-un grup de barbați simptomatici utilizand probe de urina si tehnica PCR

2015 ◽  
Vol 23 (3) ◽  
pp. 323-331
Author(s):  
Mihaela Laura Vica ◽  
Lia Monica Junie ◽  
Alecsandra Iulia Grad ◽  
Alexandru Tataru ◽  
Horea Vladi Matei
Keyword(s):  
Sexually Transmitted Diseases ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Simultaneous Detection ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Urine Samples ◽  
Multiple Infections ◽  
Sexually Transmitted ◽  
Wide Scale

Abstract Sexually transmitted diseases (STDs) are a very important cause of illness worldwide and prolonged, untreated infections with STD pathogens may have serious consequences. Our study aims to evaluate the distribution of six different STDs (Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium) in male urine samples. First void urine samples from 52 symptomatic patients were collected between April 2014 and April 2015. DNA was extracted, purified and amplified via multiplex polymerase chain reaction (PCR) for the detection of the six STD pathogens, further identified using a 2% agarose gel electrophoresis with ethidium bromide as staining agent. STD frequency in the study group was 53.84 % (28 patients), mostly in the 20-29 years age group. Among positive patients, six presented multiple infections. 35 positive DNA samples were identified in the study: 17 of C. trachomatis, 9 of U. urealyticum, 7 of N. gonorrhoeae and 2 of M. genitalium. Wide scale application of the system based on the simultaneous detection of these six pathogens inducing STD may facilitate diagnosis, especially in multiple infections.

scholarly journals Clinical Evaluation of the Cepheid Xpert TV Assay for Detection of Trichomonas vaginalis with Prospectively Collected Specimens from Men and Women

2017 ◽  
Vol 56 (2) ◽  
Author(s):  
Jane R. Schwebke ◽  
C. A. Gaydos ◽  
T. Davis ◽  
J. Marrazzo ◽  
D. Furgerson ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Transmitted Disease ◽  
The United States ◽  
Nucleic Acid Amplification ◽  
Urine Samples ◽  
Broth Culture ◽  
Men And Women ◽  
Content Type ◽  
Sexually Transmitted ◽  
Diagnostic Sensitivity And Specificity

ABSTRACT Trichomoniasis is the most prevalent curable sexually transmitted disease (STD). It has been associated with preterm birth and the acquisition and transmission of HIV. Recently, nucleic acid amplification tests (NAAT) have been FDA cleared in the United States for detection of Trichomonas vaginalis in specimens from both women and men. This study reports the results of a multicenter study recently conducted using the Xpert TV (T. vaginalis) assay to test specimens from both men and women. On-demand results were available in as little as 40 min for positive specimens. A total of 1,867 women and 4,791 men were eligible for inclusion in the analysis. In women, the performance of the Xpert TV assay was compared to the patient infected status (PIS) derived from the results of InPouch TV broth culture and Aptima NAAT for T. vaginalis. The diagnostic sensitivities and specificities of the Xpert TV assay for the combined female specimens (urine samples, self-collected vaginal swabs, and endocervical swabs) ranged from 99.5 to 100% and 99.4 to 99.9%, respectively. For male urine samples, the diagnostic sensitivity and specificity were 97.2% and 99.9%, respectively, compared to PIS results derived from the results of broth culture for T. vaginalis and bidirectional gene sequencing of amplicons. Excellent performance characteristics were seen using both female and male specimens. The ease of using the Xpert TV assay should result in opportunities for enhanced screening for T. vaginalis in both men and women and, hopefully, improved control of this infection.

scholarly journals Prospective evaluation study on the benefit of the simultaneous detection of seven sexually transmitted pathogens for the clinical management of patients suffering from sexually transmitted diseases

2019 ◽  
Vol 43 (1) ◽  
pp. 13-20
Author(s):  
Nicole Wendt ◽  
Jörg Tittelbach ◽  
Marc-Oliver Grimm ◽  
Cornelia Scheungraber ◽  
Bettina Löffler ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Simultaneous Detection ◽  
Public Health Problem ◽  
Mycoplasma Hominis ◽  
Disease Diagnosis ◽  
Diagnostic Tools ◽  
Predictive Values ◽  
Sexually Transmitted ◽  
Detection Assay

Abstract Background Sexually transmitted infections (STIs) represent a growing relevant public health problem. Early and accurate STI diagnosis is capable of preventing the spread and severe complications of curable STIs through pathogen adapted antibiotic treatment regimens. Limitations of current STI diagnostic tools are the lack of simultaneous pathogen detection and result quantification. Methods A prospective analysis of clinical specimens (n=100, from 82 symptomatic patients) from different sites of infections was performed. All samples were processed with multiplex real-time polymerase chain reaction (PCR) assay Anyplex™ II STI-7, using thermal cycler CFX96™. The Anyplex™ II STI-7 detection assay covers Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Mycoplasma genitalium (MG), Mycoplasma hominis (MH), Ureaplasma parvum (UP), Ureaplasma urealyticum (UU) and Trichomonas vaginalis (TV). STI-7 results were compared to standard methods and transferred to the attending physician for treatment adjustment. Results Compared to performed standard method results the assay achieved sensitivities ranging from 90.9% to 100%, respectively, and specificities of 100%, with negative predictive values (NPV) ranging from 98.5% to 100%, respectively, and positive predictive values (PPV) of 100%. The Anyplex™ II STI-7 detection assay measured a Cohen’s kappa of 1.00 for UU/UP, MH, and MG and a Cohen’s κ agreement of 0.95 and 0.96 for NG and CT, respectively. Conclusions The Anyplex™ II STI-7 assay can easily be introduced into the microbiological laboratory work flow due to its short hands-on-time and PCR mutiplexity. The simultaneous detection of seven STI pathogens provides a comprehensive profile for each patient, enabling clinicians to decide on best treatment options, decreasing antibiotic misuse and infection spreading risk. The semi-quantitative results enables clinicians to gain a complete package of diagnostic information including disease diagnosis, disease degree severity and treatment monitoring, although. Further clinical studies on this topic are needed.

scholarly journals MYCO WELL D-ONE detection of Ureaplasma spp. and Mycoplasma hominis in sexual health patients in Wales

2020 ◽  
Vol 39 (12) ◽  
pp. 2427-2440 ◽  
Author(s):  
Daniel J. Morris ◽  
Lucy C. Jones ◽  
Rebecca L. Davies ◽  
Kirsty Sands ◽  
Edward Portal ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Sexual Health ◽  
Sensitivity And Specificity ◽  
Tetracycline Resistance ◽  
Mycoplasma Hominis ◽  
Urine Samples ◽  
Single Individual ◽  
Sexually Transmitted ◽  
Sexual Health Clinics ◽  
Resistance Rates

AbstractThe genital mycoplasmas are a unique group of inherently antibiotic-resistant sexually transmitted bacteria, often associated with non-gonococcal urethritis and bacterial vaginosis. The MYCO WELL D-ONE is a culture-based assay that aims to detect these organisms whilst concurrently screening them for antibiotic resistance. Urine and/or swabs from 856 informed and consented participants attending Welsh sexual health clinics were subjected to MYCO WELL D-ONE analysis, alongside qPCR and culture titration methodologies to determine sensitivity, specificity, PPV, NPV and accuracy. Resistance was confirmed by CLSI-compliant susceptibility testing and genetic mechanisms determined. The MYCO WELL D-ONE displayed a sensitivity and specificity of 91.98% and 96.44% for the detection of Ureaplasma spp., with sensitivity and specificity values of 78.23% and 98.84% for Mycoplasma hominis, compared with qPCR. Swabs harboured significantly greater bacterial loads than urine samples for both Ureaplasma spp. and M. hominis. Levofloxacin resistance rates, mediated by Ser83Leu mutation in ParC, for Ureaplasma spp. were 0.54%. Tetracycline resistance rates, mediated by tet(M), were 0.54% and 2% for Ureaplasma spp. and M. hominis, respectively; sequence analysis of tet(M)-positive Ureaplasma spp. and M. hominis strains isolated from a single individual confirmed separate resistance gene origins. The MYCO WELL D-ONE is a sensitive and specific assay for the detection of Ureaplasma spp. and M. hominis in genitourinary medicine samples, facilitating the accurate detection of these organisms within low-technology environments. While good for antibiotic resistance screening, accurate confirmation by MIC determination or molecular methods are required, and more optimally performed on urine samples.

scholarly journals Prospective evaluation study on the benefit of the simultaneous detection of seven sexually transmitted pathogens for the clinical management of patients suffering from sexually transmitted diseases

2018 ◽  
Vol 0 (0) ◽  
Author(s):  
Nicole Wendt ◽  
Jörg Tittelbach ◽  
Marc-Oliver Grimm ◽  
Cornelia Scheungraber ◽  
Bettina Löffler ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Simultaneous Detection ◽  
Public Health Problem ◽  
Mycoplasma Hominis ◽  
Disease Diagnosis ◽  
Diagnostic Tools ◽  
Predictive Values ◽  
Sexually Transmitted ◽  
Detection Assay

AbstractBackgroundSexually transmitted infections (STIs) represent a growing relevant public health problem. Early and accurate STI diagnosis is capable of preventing the spread and severe complications of curable STIs through pathogen adapted antibiotic treatment regimens. Limitations of current STI diagnostic tools are the lack of simultaneous pathogen detection and result quantification.MethodsA prospective analysis of clinical specimens (n=100, from 82 symptomatic patients) from different sites of infections was performed. All samples were processed with multiplex real-time polymerase chain reaction (PCR) assay Anyplex™ II STI-7, using thermal cycler CFX96™. The Anyplex™ II STI-7 detection assay coversChlamydia trachomatis(CT),Neisseria gonorrhoeae(NG),Mycoplasma genitalium(MG),Mycoplasma hominis(MH),Ureaplasma parvum(UP),Ureaplasma urealyticum(UU) andTrichomonas vaginalis(TV). STI-7 results were compared to standard methods and transferred to the attending physician for treatment adjustment.ResultsCompared to performed standard method results the assay achieved sensitivities ranging from 90.9% to 100%, respectively, and specificities of 100%, with negative predictive values (NPV) ranging from 98.5% to 100%, respectively, and positive predictive values (PPV) of 100%. The Anyplex™ II STI-7 detection assay measured a Cohen’s kappa of 1.00 for UU/UP, MH, and MG and a Cohen’s κ agreement of 0.95 and 0.96 for NG and CT, respectively.ConclusionsThe Anyplex™ II STI-7 assay can easily be introduced into the microbiological laboratory work flow due to its short hands-on-time and PCR mutiplexity. The simultaneous detection of seven STI pathogens provides a comprehensive profile for each patient, enabling clinicians to decide on best treatment options, decreasing antibiotic misuse and infection spreading risk. The semi-quantitative results enables clinicians to gain a complete package of diagnostic information including disease diagnosis, disease degree severity and treatment monitoring, although. Further clinical studies on this topic are needed.

scholarly journals Combined Testing for Chlamydia, Gonorrhea, and Trichomonas by Use of the BD Max CT/GC/TV Assay with Genitourinary Specimen Types

2016 ◽  
Vol 55 (1) ◽  
pp. 155-164 ◽  
Author(s):  
Barbara Van Der Pol ◽  
James A. Williams ◽  
DeAnna Fuller ◽  
Stephanie N. Taylor ◽  
Edward W. Hook
Keyword(s):  
Simultaneous Detection ◽  
Urine Samples ◽  
Transmitted Infection ◽  
Content Type ◽  
Male Urine ◽  
Sexually Transmitted ◽  
Sti Testing ◽  
Vaginal Swabs ◽  
Female Urine ◽  
Urine Specimens

ABSTRACT The BD Max CT/GC/TV (MAX) assay is a true multiplex assay for simultaneous detection of chlamydia (CT), gonorrhea (GC), and trichomonas (TV). We evaluated assay performance for women using endocervical and vaginal swabs as well as urine specimens. A total of 1,143 women were tested for CT, GC, and TV and, subsequently, another 847 (1,990 total women) for CT and GC only, with positivity rates for CT, GC, and TV of 7.1%, 2.3%, and 13.5%, respectively. In men, the performance for CT and GC was determined using only urine specimens. TV performance was not assessed in male urine samples. Among men, 181/830 (21.8%) and 108/840 (12.9%) chlamydia and gonorrhea infections, respectively, were identified. Comparator assays included BD ProbeTec Chlamydia trachomatis Q x (CTQ)/ Neisseria gonorrhoeae Q x (GCQ), Hologic Aptima Combo 2 (AC2) and Aptima TV (ATV), trichomonas microscopy, and culture. MAX CT sensitivity was 99.3% (95% confidence interval [CI], 96.1% to 99.9%), 95.7% (90.8% to 98.0%), 91.5% (85.8% to 95.1%), and 96.1% (92.2% to 98.1%) for vaginal swabs, endocervical swabs, female urine samples, and male urine samples, respectively. MAX GC sensitivity was 95.5% (84.9% to 98.7%), 95.5% (84.9% to 98.7%), 95.7% (85.5% to 99.8%), and 99.1% (94.9% to 99.8%) in the same order. MAX TV sensitivity was 96.1% (91.7% to 98.2%) for vaginal swabs, 93.4% (88.3% to 96.4%) for endocervical swabs, and 92.9% (87.8% to 96.0%) for female urine samples. Specificity for all organisms across all sample types was ≥98.6%. Performance estimates for the MAX assays were consistent with estimates calculated for the comparator assays (all P values were >0.1). The availability of a CT/GC/TV multiplexed assay on a benchtop instrument with a broad menu has the potential to facilitate local sexually transmitted infection (STI) testing at smaller laboratories and may encourage expanded screening for these highly prevalent infections.

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