Elliptochloris bilobata var. corticola var. nov. (Trebouxiophyceae, Chlorophyta), a novel subaerial coccal green alga

Biologia ◽  
2008 ◽  
Vol 63 (6) ◽  
Author(s):  
Marek Eliáš ◽  
Jiří Neustupa ◽  
Pavel Škaloud
Keyword(s):  
Cell Wall ◽  
18S Rdna ◽  
Type Species ◽  
Gene Sequence ◽  
Morphological Characters ◽  
Chloroplast Structure ◽  
Monophyletic Clade ◽  
Green Algal ◽  
Globular Cells ◽  
18S Rdna Gene

AbstractWe investigated a previously unidentified subaerial corticolous strain of the genus Elliptochloris Tschermak-Woess. The alga shares the generic morphological characters with Elliptochloris bilobata, the type species of the genus, but it has a thicker cell wall of adult globular cells, different chloroplast structure and it also differs in shape of elliptical autospores. The differences of the autospore shape between both species were evaluated using landmark-based geometric morphometrics. The 18S rDNA gene sequence of the new alga forms a monophyletic clade with the authentic strain of E. bilobata within the green algal class Trebouxiophyceae close to representatives of the genus Coccomyxa. We describe the new alga as Elliptochloris bilobata var. corticola var. nov.

Lysinibacillus manganicus sp. nov., isolated from manganese mining soil

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3568-3573 ◽  
Author(s):  
Hongliang Liu ◽  
Yumei Song ◽  
Fang Chen ◽  
Shixue Zheng ◽  
Gejiao Wang
Keyword(s):  
Cell Wall ◽  
Dna Hybridization ◽  
Type Species ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Content Type ◽  
Mining Soil ◽  
Link Type ◽  
Type Strains

A Gram-stain-positive, aerobic, motile, rod-shaped bacterium, designated strain Mn1-7T, was isolated from manganese mining soil in Tianjin, China. The closest phylogenetic relatives were Lysinibacillus massiliensis CCUG 49529T (97.2 % 16S rRNA gene sequence similarity), L. xylanilyticus XDB9T (96.7 %), L. sinduriensis JCM 15800T (96.2 %), L. odysseyi NBRC 100172T (95.9 %) and L. boronitolerans NBRC 103108T (95.4 %) (the type species of the genus). DNA–DNA hybridization values for strain Mn1-7T with the type strains of L. massiliensis and L. sinduriensis were 24.9 and 27.7 %, respectively. The genomic DNA G+C content was 38.4 mol%. The major menaquinone was MK-7 and the major fatty acids were iso-C15 : 0, iso-C16 : 0 and iso-C14 : 0. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The cell-wall peptidoglycan was type A4α (l-Lys–d-Asp), and the predominant cell-wall sugar was xylose. DNA–DNA hybridization results and comparison of phenotypic and chemotaxonomic characters between strain Mn1-7T and the phylogenetically most closely related strains revealed that the isolate represents a novel species of the genus Lysinibacillus , for which the name Lysinibacillus manganicus sp. nov. is proposed. The type strain is Mn1-7T ( = DSM 26584T = CCTCC AB 2012916T).

scholarly journals Rhizocola hellebori gen. nov., sp. nov., an actinomycete of the family Micromonosporaceae containing 3,4-dihydroxydiaminopimelic acid in the cell-wall peptidoglycan

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2706-2711 ◽  
Author(s):  
Atsuko Matsumoto ◽  
Yoko Kawaguchi ◽  
Takuji Nakashima ◽  
Masato Iwatsuki ◽  
Satoshi Ōmura ◽  
...  
Keyword(s):  
Cell Wall ◽  
Type Species ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
Cell Wall Peptidoglycan ◽  
The Family ◽  
The 16S Rrna Gene

An actinomycete strain, K12-0602T, was isolated from the root of a Helleborus orientalis plant in Japan. The 16S rRNA gene sequence of strain K12-0602T showed that it had a close relationship with members of the family Micromonosporaceae and the 16S rRNA gene sequence similarity values between strain K12-0602T and type strains of type species of 27 genera belonging to the family Micromonosporaceae were below 96.2 %. MK-9 (H4) and MK-9 (H6) were detected as major menaquinones, and galactose, xylose, mannose and ribose were present in the whole-cell hydrolysate. The acyl type of the peptidoglycan was glycolyl. Major fatty acids were iso-C15 : 0, iso-C16 : 0, C17 : 1ω9c and anteiso-C17 : 0. Phosphatidylethanolamine was detected as the phospholipid corresponding to phospholipid type II. The G+C content of the genomic DNA was 67 mol%. Analyses of the cell-wall peptidoglycan by TLC and LC/MS showed that it was composed of alanine, glycine, hydroxylglutamic acid and an unknown amino acid, which was subsequently determined to be 3,4-dihydroxydiaminopimelic acid using instrumental analyses, including NMR and mass spectrometry. On the basis of the phylogenetic analysis and chemotaxonomic characteristics, strain K12-0602T represents a novel species of a new genus in the family Micromonosporaceae , for which the name Rhizocola hellebori gen. nov., sp. nov. is proposed. The type strain of the type species is K12-0602T ( = NBRC 109834T = DSM 45988T). This is the first report, to our knowledge, of 3,4-dihydroxydiaminopimelic acid being found as a diamino acid in bacterial cell-wall peptidoglycan.

Morphology and 18S rDNA gene sequence of Blepharisma sinuosum Sawaya, 1940 (Ciliophora: Heterotrichea) from Brazil

2013 ◽  
Vol 49 (4) ◽  
pp. 623-633 ◽  
Author(s):  
Noemi Mendes Fernandes ◽  
Roberto Júnio Pedroso Dias ◽  
Marcus Vinicius Xavier Senra ◽  
Carlos Augusto Gomes Soares ◽  
Inácio Domingos da Silva Neto
Keyword(s):  
18S Rdna ◽  
Gene Sequence ◽  
Rdna Gene ◽  
18S Rdna Gene

Morphological and molecular characterisation of Moshajia idiofora Siddiqi, 1982 (Dorylaimida: Qudsianematidae), with new insights into the phylogeny of the enigmatic Lordellonematinae

Nematology ◽  
2019 ◽  
Vol 21 (4) ◽  
pp. 349-360 ◽  
Author(s):  
Zarrin Imran ◽  
Joaquín Abolafia ◽  
Reyes Peña-Santiago ◽  
Wasim Ahmad
Keyword(s):  
Sequence Analysis ◽  
18S Rdna ◽  
Gene Sequence ◽  
Molecular Data ◽  
Morphological Description ◽  
Molecular Characterisation ◽  
Rdna Gene ◽  
Gene Sequence Analysis ◽  
18S Rdna Gene ◽  
Region Form

Summary Seven populations of Moshajia idiofora, six from India and one from Japan, are characterised, including morphological description, morphometrics, LM and SEM observations, and molecular (18S rDNA gene sequence) analysis. These populations are compared to previous records of the species as well as its closest species. Two of these species, M. cultristyla and M. qasimi, are regarded as junior synonyms of M. idiofora. SEM pictures provide new details of the lip region form. Molecular data indicate that M. idiofora belongs to a morphologically heterogeneous clade whose components share the absence of a distinctly developed pars refringens vaginae and raise a serious doubt about the traditional position of Lordellonematinae representatives in the Dorylaimina system.

Amycolatopsis dendrobii sp. nov., an endophytic actinomycete isolated from Dendrobium heterocarpum Lindl.

2021 ◽  
Vol 71 (7) ◽  
Author(s):  
Nisachon Tedsree ◽  
Somboon Tanasupawat ◽  
Boonchoo Sritularak ◽  
Nattakorn Kuncharoen ◽  
Kittisak Likhitwitayawuid
Keyword(s):  
Cell Wall ◽  
Type Species ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Taxonomic Approach ◽  
Genotypic Characteristics

Three novel actinomycete strains, designated as DR6-1T, DR6-2 and DR6-4, isolated from the roots of Dendrobium heterocarpum Lindl in Thailand were studied using a polyphasic taxonomic approach. The strains grew at 20–37 °C, at pH 5–10 and with 5 % (w/v) NaCl. They contained meso-diaminopimelic acid in the cell-wall peptidoglycan and MK-9(H4) was a major menaquinone. Arabinose and galactose were the major sugars in the cell wall. The predominant cellular fatty acids were iso-C16 : 0 and iso-C15 : 0. The detected polar lipids were diphosphatidylglycerol, hydroxyphosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylglycerol. Strains DR6-1T, DR6-2 and DR6-4 shared 99.9–100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%). The approximate genome size of strain DR6-1T was 9.6 Mb with a G+C content of 69.6 mol%. The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively. Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1T, DR6-2 and DR6-4 represent a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp. nov. is proposed. The type strain is DR6-1T (=JCM 33742T=KCTC 49546T=TISTR 2840T).

Brevibacterium siliguriense sp. nov., a facultatively oligotrophic bacterium isolated from river water

2013 ◽  
Vol 63 (Pt_2) ◽  
pp. 511-515 ◽  
Author(s):  
Arvind Kumar ◽  
İkbal Agah İnce ◽  
Ahmet Katı ◽  
Ranadhir Chakraborty
Keyword(s):  
Cell Wall ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A Gram-positive-staining, rod-shaped, facultatively oligotrophic bacterial strain, designated MB18T, was isolated from a water sample collected from the River Mahananda at Siliguri (26° 44′ 23.20′ N, 88° 25′ 22.89′ E), West-Bengal, India. On the basis of 16S rRNA gene sequence similarity, the closest relative of this strain was Brevibacterium epidermidis NCDO 2286T (96 % similarity). The DNA G+C content of strain MB18T was 64.6 mol%. Chemotaxonomic data [MK-8(H2) as the major menaquinone, galactose as the sole cell-wall sugar, meso-diaminopimelic acid as the diagnostic cell-wall diamino acid, phosphatidylglycerol and diphosphatidylglycerol as constituents of the polar lipids, anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0 as the major fatty acids] supported the affiliation of strain MB18T to the genus Brevibacterium . The results of DNA G+C content, 16S rRNA gene sequence analysis and biochemical and physiological analyses allowed genotypic and phenotypic differentiation of strain MB18T from its nearest neighbour B. epidermidis . The isolate therefore represents a novel species, for which the name Brevibacterium siliguriense sp. nov. is proposed; the type strain is MB18T ( = DSM 23676T = LMG 25772T).

Phylogenetic relationships in the genus Closterium

2021 ◽  
pp. 151-154
Author(s):  
Nikita A. Martynenko ◽  
Evgeniy S. Gusev
Keyword(s):  
Phylogenetic Relationships ◽  
18S Rdna ◽  
Molecular Genetic ◽  
Taxonomic Diversity ◽  
Morphological Characters ◽  
Monophyletic Clade ◽  
Genetic Studies ◽  
Species Complexes ◽  
The Family ◽  
General Tree

Desmids are a large polyphyletic group of streptophyte algae, numbering about 4000 species. Traditionally, the taxonomy of desmids is based on morphological characters. However, the latest molecular genetic studies of the group conflict with the established taxonomy and indicate the polyphyletic position of some genera of this group of algae. The genus Closterium Nitzsch ex Ralfs, like the family Closteriaceae Bessey, forms a monophyletic clade on the general tree of Desmidiales, recovered on the data of both 18S rDNA and rbcL cpDNA. In our analysis of phylogenetic relationships within the genus Closterium, the existing strains formed 14 clades (species complexes). Within some complexes, some strains don’t differ phenotypically, but diverge on the phylogenetic tree, which indicates a hidden taxonomic diversity within the genus.

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